Burkholderia virus Bcep22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lessievirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6V7P1|Q6V7P1_9CAUD Uncharacterized protein OS=Burkholderia virus Bcep22 OX=242527 GN=Bcep22_gp52 PE=4 SV=1
MM1 pKa = 6.28QTYY4 pKa = 9.17IANPVRR10 pKa = 11.84VSATRR15 pKa = 11.84IVDD18 pKa = 3.44VGMTAEE24 pKa = 4.36EE25 pKa = 4.55SPSGRR30 pKa = 11.84MVTPLALEE38 pKa = 4.81DD39 pKa = 3.65GTNFVADD46 pKa = 3.26SGMTARR52 pKa = 11.84YY53 pKa = 8.35FPQAGDD59 pKa = 3.41YY60 pKa = 11.02LVIQEE65 pKa = 5.87DD66 pKa = 5.44GYY68 pKa = 11.48QYY70 pKa = 11.31LNPRR74 pKa = 11.84EE75 pKa = 4.11VFEE78 pKa = 4.14RR79 pKa = 11.84KK80 pKa = 9.33YY81 pKa = 10.86RR82 pKa = 11.84LLVLTDD88 pKa = 3.8ADD90 pKa = 4.03ALADD94 pKa = 3.91LKK96 pKa = 11.51GEE98 pKa = 4.13PRR100 pKa = 11.84PDD102 pKa = 3.32NPTSS106 pKa = 3.26

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6V7Q3|Q6V7Q3_9CAUD Uncharacterized protein OS=Burkholderia virus Bcep22 OX=242527 GN=Bcep22_gp40 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.42AMSILASIIGASRR15 pKa = 11.84RR16 pKa = 11.84FFTPAPSFAIKK27 pKa = 10.25RR28 pKa = 11.84IKK30 pKa = 10.37APRR33 pKa = 11.84TSTRR37 pKa = 11.84STTAGVKK44 pKa = 9.77RR45 pKa = 11.84AAAKK49 pKa = 9.6ARR51 pKa = 11.84NVKK54 pKa = 9.07RR55 pKa = 11.84HH56 pKa = 4.76KK57 pKa = 10.53AAMRR61 pKa = 11.84RR62 pKa = 11.84AAA64 pKa = 4.3

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

20072

43

4602

260.7

28.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.033 ± 0.433

0.892 ± 0.215

6.482 ± 0.206

6.143 ± 0.388

3.004 ± 0.165

8.091 ± 0.361

1.853 ± 0.18

4.663 ± 0.245

4.514 ± 0.269

7.339 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.142

3.428 ± 0.19

5.321 ± 0.185

4.026 ± 0.299

7.498 ± 0.381

5.231 ± 0.212

5.764 ± 0.207

6.337 ± 0.24

1.39 ± 0.11

2.411 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski