Paenibacillus sp. TCA20

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5226 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A069DAF2|A0A069DAF2_9BACL UPF0060 membrane protein TCA2_1843 OS=Paenibacillus sp. TCA20 OX=1499968 GN=TCA2_1843 PE=3 SV=1
MM1 pKa = 6.85QSKK4 pKa = 9.78PIRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84LMSLSVVSTLFLSTVFPAVVSGAEE33 pKa = 3.7ATNRR37 pKa = 11.84KK38 pKa = 8.94FDD40 pKa = 3.94PSLFNLDD47 pKa = 3.38EE48 pKa = 4.76SKK50 pKa = 11.16LNSQMNGVTLNSSKK64 pKa = 10.66KK65 pKa = 9.4QAALNNTDD73 pKa = 2.7QSTNTGSKK81 pKa = 9.64IEE83 pKa = 5.09SYY85 pKa = 8.95TQSDD89 pKa = 3.34NSEE92 pKa = 4.1RR93 pKa = 11.84ASSVITPQNYY103 pKa = 8.99VPEE106 pKa = 4.65SDD108 pKa = 3.61STSTISVIVEE118 pKa = 3.95LSEE121 pKa = 4.2EE122 pKa = 4.53PIALYY127 pKa = 10.15EE128 pKa = 4.47SKK130 pKa = 10.71SSQNLNSGNNDD141 pKa = 2.99QRR143 pKa = 11.84SLIQKK148 pKa = 7.16QQQSFASAAKK158 pKa = 10.2RR159 pKa = 11.84LNATLKK165 pKa = 9.01TPYY168 pKa = 10.15SVVFNGYY175 pKa = 10.13AVDD178 pKa = 3.45IRR180 pKa = 11.84ADD182 pKa = 3.8RR183 pKa = 11.84VEE185 pKa = 4.36SLLAIPGVKK194 pKa = 9.52AVYY197 pKa = 9.59PDD199 pKa = 4.26SEE201 pKa = 4.48MKK203 pKa = 9.38ATPINSITPNMDD215 pKa = 2.56EE216 pKa = 4.12SAPFIGSDD224 pKa = 3.54TFWDD228 pKa = 3.09IGYY231 pKa = 9.67DD232 pKa = 3.45GAGIKK237 pKa = 10.5VGVLDD242 pKa = 3.48TGIDD246 pKa = 3.75YY247 pKa = 7.96EE248 pKa = 4.58HH249 pKa = 7.6PSLADD254 pKa = 3.69AYY256 pKa = 10.02KK257 pKa = 10.64GGYY260 pKa = 10.07DD261 pKa = 5.42FIDD264 pKa = 4.08NDD266 pKa = 4.21DD267 pKa = 4.94DD268 pKa = 4.75PYY270 pKa = 10.84EE271 pKa = 4.44TPPDD275 pKa = 4.27PNDD278 pKa = 3.55PEE280 pKa = 4.44AATDD284 pKa = 3.42HH285 pKa = 5.97GTHH288 pKa = 5.88VSGTIAGRR296 pKa = 11.84GNPLDD301 pKa = 4.36PEE303 pKa = 4.69SGTGWVRR310 pKa = 11.84GVAYY314 pKa = 10.04GADD317 pKa = 2.85IYY319 pKa = 10.95AYY321 pKa = 9.87RR322 pKa = 11.84VLGPGGSGPTSGVIAAIEE340 pKa = 3.89QSVEE344 pKa = 3.81DD345 pKa = 5.38DD346 pKa = 3.17MDD348 pKa = 5.01IINLSLGSEE357 pKa = 4.4MNNEE361 pKa = 4.06FDD363 pKa = 4.01PASVALNNAALAGVVAVVANGNSGPDD389 pKa = 3.92DD390 pKa = 3.79YY391 pKa = 11.84TLGSPGAAEE400 pKa = 4.16IPISVGASTPPLNVPTVDD418 pKa = 4.0GEE420 pKa = 4.61GLLTSYY426 pKa = 10.97GSLMTFSPDD435 pKa = 3.39LSTLDD440 pKa = 3.64GEE442 pKa = 4.78TIEE445 pKa = 4.49VVYY448 pKa = 10.85AGLGTTDD455 pKa = 3.0EE456 pKa = 4.55TEE458 pKa = 4.39AVDD461 pKa = 3.86LTGKK465 pKa = 9.86VALIQRR471 pKa = 11.84GSISFTEE478 pKa = 4.21KK479 pKa = 10.59SVNAQAAGAVAAIIYY494 pKa = 10.27NNEE497 pKa = 3.87PGNFGGTLGVPGDD510 pKa = 4.32YY511 pKa = 10.55IPTLSISLEE520 pKa = 4.31DD521 pKa = 3.54GTALKK526 pKa = 10.64SKK528 pKa = 10.64IEE530 pKa = 3.95ATPGYY535 pKa = 9.46EE536 pKa = 3.53ITFGIDD542 pKa = 3.1LQQDD546 pKa = 3.62LMADD550 pKa = 4.39FSSRR554 pKa = 11.84GPALPKK560 pKa = 10.65YY561 pKa = 9.93GIKK564 pKa = 9.95PDD566 pKa = 3.22ISAPGVAIRR575 pKa = 11.84SSIPAYY581 pKa = 10.35GGDD584 pKa = 3.63YY585 pKa = 10.97SEE587 pKa = 6.02AYY589 pKa = 10.4ADD591 pKa = 3.86FQGTSMATPHH601 pKa = 5.67VAGAAALLLDD611 pKa = 4.43KK612 pKa = 10.85DD613 pKa = 3.93ASLTLSEE620 pKa = 4.84IKK622 pKa = 10.92GLMTNNALKK631 pKa = 10.89LSDD634 pKa = 4.24LDD636 pKa = 3.85GNRR639 pKa = 11.84YY640 pKa = 6.73EE641 pKa = 5.52HH642 pKa = 6.98NIQGAGRR649 pKa = 11.84LDD651 pKa = 3.76LEE653 pKa = 4.53STLEE657 pKa = 4.05AKK659 pKa = 10.26AVALVQEE666 pKa = 4.34EE667 pKa = 4.63TTAVEE672 pKa = 4.47SGEE675 pKa = 4.06EE676 pKa = 4.06TPYY679 pKa = 10.17EE680 pKa = 4.21TGLLSYY686 pKa = 10.7GILDD690 pKa = 4.16GGSTSTKK697 pKa = 8.99TIEE700 pKa = 4.08VSDD703 pKa = 3.95VVGEE707 pKa = 4.14GSSYY711 pKa = 10.99SVSTEE716 pKa = 3.61WYY718 pKa = 6.5GTPPGTLSVSEE729 pKa = 4.41SSISVPVNGEE739 pKa = 3.4ASIDD743 pKa = 3.48VTIAMSNGIADD754 pKa = 3.76GNYY757 pKa = 9.38EE758 pKa = 4.1GEE760 pKa = 4.92LILTEE765 pKa = 4.55AGGHH769 pKa = 4.53QLKK772 pKa = 10.42IPVYY776 pKa = 8.6VYY778 pKa = 10.08VGEE781 pKa = 4.07PVEE784 pKa = 4.31IPVVSDD790 pKa = 3.4VAVDD794 pKa = 3.72PQFFSPNEE802 pKa = 4.03DD803 pKa = 3.1EE804 pKa = 5.8LYY806 pKa = 10.54DD807 pKa = 3.72YY808 pKa = 10.96TDD810 pKa = 3.2VYY812 pKa = 11.15FSVNAGNEE820 pKa = 4.14KK821 pKa = 10.11IALDD825 pKa = 3.46ILNPNGKK832 pKa = 8.09WLGSIDD838 pKa = 3.71EE839 pKa = 4.98SNGEE843 pKa = 4.35MEE845 pKa = 5.56PGDD848 pKa = 3.78YY849 pKa = 10.04WIQQWDD855 pKa = 3.72GTIINGPNIIKK866 pKa = 10.59LEE868 pKa = 4.35DD869 pKa = 4.31GIYY872 pKa = 9.78FASGSALINGGWIEE886 pKa = 4.35YY887 pKa = 10.34LDD889 pKa = 3.57EE890 pKa = 5.28AYY892 pKa = 10.02PFVLDD897 pKa = 3.77TAAPVSTLDD906 pKa = 3.83DD907 pKa = 3.73PQITVEE913 pKa = 4.32GDD915 pKa = 2.82VGTIRR920 pKa = 11.84GQIEE924 pKa = 4.23SDD926 pKa = 3.72LMVDD930 pKa = 3.8ALGNYY935 pKa = 9.76SGIGVEE941 pKa = 4.83AGYY944 pKa = 10.59LVDD947 pKa = 5.62GEE949 pKa = 3.9WDD951 pKa = 3.67YY952 pKa = 11.99AIGSISDD959 pKa = 3.37NGEE962 pKa = 3.56FTIEE966 pKa = 3.96VPIVEE971 pKa = 4.34GQNDD975 pKa = 4.01FEE977 pKa = 5.24VYY979 pKa = 10.52VYY981 pKa = 10.45DD982 pKa = 3.59YY983 pKa = 9.9TGNGFIEE990 pKa = 4.74PSHH993 pKa = 5.79LVSYY997 pKa = 10.35EE998 pKa = 3.89SDD1000 pKa = 3.44GEE1002 pKa = 4.36EE1003 pKa = 4.28PGEE1006 pKa = 3.99PTPGEE1011 pKa = 3.61ISLTVDD1017 pKa = 3.68PSADD1021 pKa = 2.99EE1022 pKa = 4.28VGVNEE1027 pKa = 5.11SFNLDD1032 pKa = 2.85IDD1034 pKa = 4.16FSEE1037 pKa = 5.18VEE1039 pKa = 4.58DD1040 pKa = 4.57LYY1042 pKa = 11.51SAQFSLTYY1050 pKa = 10.79DD1051 pKa = 3.46EE1052 pKa = 5.82DD1053 pKa = 4.12LVKK1056 pKa = 10.31GTTAPSVVLATYY1068 pKa = 9.55QEE1070 pKa = 4.25EE1071 pKa = 4.41QNPGVTPYY1079 pKa = 10.71VYY1081 pKa = 10.82EE1082 pKa = 4.01EE1083 pKa = 4.08TTALGNGLAKK1093 pKa = 9.51TDD1095 pKa = 3.79YY1096 pKa = 11.05VITLVGLEE1104 pKa = 3.93SGYY1107 pKa = 9.5TGEE1110 pKa = 4.61GALASFNFAGVSEE1123 pKa = 4.4GEE1125 pKa = 4.03YY1126 pKa = 10.84DD1127 pKa = 3.9FEE1129 pKa = 5.06LSNVRR1134 pKa = 11.84ALNSTSADD1142 pKa = 3.1IAVGDD1147 pKa = 3.58ITGASVTVTSDD1158 pKa = 3.59PSPGQYY1164 pKa = 10.4SITGSIDD1171 pKa = 2.93AEE1173 pKa = 4.14AFADD1177 pKa = 4.04GVDD1180 pKa = 4.61YY1181 pKa = 11.6SEE1183 pKa = 4.14TWYY1186 pKa = 10.74QGADD1190 pKa = 3.27GVHH1193 pKa = 5.53KK1194 pKa = 10.76VVVEE1198 pKa = 4.14AVDD1201 pKa = 3.88SNGSVAGVGTVRR1213 pKa = 11.84ADD1215 pKa = 3.24GTYY1218 pKa = 9.83TIEE1221 pKa = 4.28VPEE1224 pKa = 3.98GTYY1227 pKa = 7.61TVRR1230 pKa = 11.84VVVPGHH1236 pKa = 4.91ITEE1239 pKa = 4.51TAGVTVDD1246 pKa = 4.38GNEE1249 pKa = 4.08TLNFGPLTAGDD1260 pKa = 4.04VNNDD1264 pKa = 3.13GAVNLVDD1271 pKa = 4.16LQLTAKK1277 pKa = 10.33EE1278 pKa = 3.95FGKK1281 pKa = 9.22TKK1283 pKa = 10.4GSAWANAKK1291 pKa = 10.36ASAADD1296 pKa = 3.51INRR1299 pKa = 11.84DD1300 pKa = 3.54SAVDD1304 pKa = 4.13LLDD1307 pKa = 3.13ISYY1310 pKa = 10.49IIGNYY1315 pKa = 7.84EE1316 pKa = 3.63LL1317 pKa = 4.92

Molecular weight: 138.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A069DFT5|A0A069DFT5_9BACL LytR_cpsA_psr domain-containing protein OS=Paenibacillus sp. TCA20 OX=1499968 GN=TCA2_3593 PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5226

0

5226

1621612

39

4464

310.3

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.689 ± 0.032

0.685 ± 0.009

5.269 ± 0.027

7.318 ± 0.04

3.977 ± 0.022

7.203 ± 0.035

2.214 ± 0.019

7.034 ± 0.033

5.296 ± 0.029

9.774 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.987 ± 0.016

4.011 ± 0.025

3.916 ± 0.024

3.964 ± 0.022

4.727 ± 0.03

6.527 ± 0.029

5.533 ± 0.035

6.945 ± 0.029

1.194 ± 0.016

3.737 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski