Candidatus Phaeomarinobacter ectocarpi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Parvibaculaceae; Candidatus Phaeomarinobacter

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3295 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5ME47|X5ME47_9RHIZ CAIB/BAIF family protein OS=Candidatus Phaeomarinobacter ectocarpi OX=1458461 GN=BN1012_Phect2479 PE=4 SV=1
MM1 pKa = 6.95TTTPDD6 pKa = 3.68GQPVPAEE13 pKa = 4.73DD14 pKa = 5.25DD15 pKa = 4.28LEE17 pKa = 6.37LDD19 pKa = 4.44DD20 pKa = 5.67EE21 pKa = 4.67LMAAIAAAEE30 pKa = 3.93AAVGDD35 pKa = 4.11MASDD39 pKa = 3.92YY40 pKa = 11.35PEE42 pKa = 4.93MLLQDD47 pKa = 3.42IGLLTGAIARR57 pKa = 11.84LDD59 pKa = 3.44LHH61 pKa = 7.3APGTDD66 pKa = 3.23EE67 pKa = 5.61HH68 pKa = 6.53ATACIDD74 pKa = 3.77CFGVLHH80 pKa = 7.18DD81 pKa = 5.06IKK83 pKa = 11.08GQAASFGYY91 pKa = 10.21EE92 pKa = 4.04LATALCDD99 pKa = 3.87PLCEE103 pKa = 4.26CVRR106 pKa = 11.84GQHH109 pKa = 5.55SASMQLIDD117 pKa = 4.2QLKK120 pKa = 8.27QAVDD124 pKa = 3.02ILSRR128 pKa = 11.84MAAEE132 pKa = 4.81GVSADD137 pKa = 3.75TDD139 pKa = 3.61NAALALIRR147 pKa = 11.84QLPDD151 pKa = 2.65AA152 pKa = 5.26

Molecular weight: 15.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5M635|X5M635_9RHIZ Transcriptional regulator TetR family OS=Candidatus Phaeomarinobacter ectocarpi OX=1458461 GN=BN1012_Phect174 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.6GFRR19 pKa = 11.84ARR21 pKa = 11.84LATKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.95VLQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3295

0

3295

1010352

37

3695

306.6

33.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.112 ± 0.056

0.781 ± 0.015

6.537 ± 0.041

6.02 ± 0.037

3.685 ± 0.027

8.488 ± 0.043

2.086 ± 0.024

5.143 ± 0.029

3.565 ± 0.039

9.457 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.025

2.799 ± 0.026

4.996 ± 0.032

3.175 ± 0.024

6.168 ± 0.04

5.553 ± 0.032

5.739 ± 0.044

7.405 ± 0.038

1.297 ± 0.016

2.245 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski