Bromus-associated circular DNA virus 2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4U8V8|A0A0B4U8V8_9VIRU Uncharacterized protein OS=Bromus-associated circular DNA virus 2 OX=1590155 PE=4 SV=1
MM1 pKa = 7.29SQFKK5 pKa = 10.98SNSIWSEE12 pKa = 4.15VPDD15 pKa = 4.1HH16 pKa = 6.98EE17 pKa = 5.9KK18 pKa = 11.2DD19 pKa = 3.28LDD21 pKa = 4.09PPVSPLSLTTTNIEE35 pKa = 4.1EE36 pKa = 4.06HH37 pKa = 5.51NLRR40 pKa = 11.84YY41 pKa = 9.42RR42 pKa = 11.84RR43 pKa = 11.84CAGPVPPSRR52 pKa = 11.84ASTQSVASDD61 pKa = 3.52EE62 pKa = 4.56SRR64 pKa = 11.84ADD66 pKa = 3.54PSLVAVLSRR75 pKa = 11.84LDD77 pKa = 3.78LQDD80 pKa = 3.65AALDD84 pKa = 4.41EE85 pKa = 4.38ILARR89 pKa = 11.84LSLLHH94 pKa = 7.01RR95 pKa = 11.84EE96 pKa = 4.68FSRR99 pKa = 11.84PLPSPIISSTTQPEE113 pKa = 4.63FSATLPPGLLWW124 pKa = 4.36

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4U8Q3|A0A0B4U8Q3_9VIRU Uncharacterized protein OS=Bromus-associated circular DNA virus 2 OX=1590155 PE=4 SV=1
MM1 pKa = 7.25VGGSRR6 pKa = 11.84SRR8 pKa = 11.84KK9 pKa = 8.53RR10 pKa = 11.84PRR12 pKa = 11.84SPGQSSVADD21 pKa = 3.89YY22 pKa = 10.26YY23 pKa = 10.88KK24 pKa = 10.49HH25 pKa = 6.91RR26 pKa = 11.84RR27 pKa = 11.84TQSPIPTMRR36 pKa = 11.84WARR39 pKa = 11.84AAFKK43 pKa = 10.58GKK45 pKa = 9.8YY46 pKa = 8.47AVRR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 10.0RR52 pKa = 11.84RR53 pKa = 11.84VTRR56 pKa = 11.84RR57 pKa = 11.84SLSRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84LKK65 pKa = 10.26PSRR68 pKa = 11.84FTRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84SRR75 pKa = 11.84RR76 pKa = 11.84NTRR79 pKa = 11.84PSKK82 pKa = 10.45SFASRR87 pKa = 11.84VLKK90 pKa = 9.61ATAVTNHH97 pKa = 7.38LIYY100 pKa = 9.88DD101 pKa = 3.75TAGILRR107 pKa = 11.84NVTTGVALVNVKK119 pKa = 10.46NPTLTQTDD127 pKa = 4.3FQAVQAKK134 pKa = 9.92LNNDD138 pKa = 3.33EE139 pKa = 4.67VLDD142 pKa = 4.06SVTNKK147 pKa = 10.11SFYY150 pKa = 10.27LRR152 pKa = 11.84HH153 pKa = 5.61ARR155 pKa = 11.84EE156 pKa = 4.07TYY158 pKa = 9.71TYY160 pKa = 10.28TNSSTNKK167 pKa = 7.46ITLKK171 pKa = 9.89IYY173 pKa = 9.96HH174 pKa = 5.88YY175 pKa = 10.4KK176 pKa = 10.78ARR178 pKa = 11.84FDD180 pKa = 4.37YY181 pKa = 11.0NISPQTLYY189 pKa = 11.18NQGFTDD195 pKa = 4.59DD196 pKa = 4.23GAIQATSVNSTPFMSNNFTTYY217 pKa = 10.56NKK219 pKa = 8.18ITRR222 pKa = 11.84VVTKK226 pKa = 9.97FLEE229 pKa = 4.51QGEE232 pKa = 4.64HH233 pKa = 3.8MTVYY237 pKa = 10.48QNQSSKK243 pKa = 10.78HH244 pKa = 5.38INSDD248 pKa = 3.27RR249 pKa = 11.84YY250 pKa = 10.46KK251 pKa = 10.8NSLVVAGSHH260 pKa = 4.56GHH262 pKa = 4.87VLFGVGTLGYY272 pKa = 8.76DD273 pKa = 3.54TVNTGITTGPVEE285 pKa = 4.53LMQRR289 pKa = 11.84LQRR292 pKa = 11.84HH293 pKa = 5.32ISYY296 pKa = 10.21SQLADD301 pKa = 3.2NTVRR305 pKa = 11.84NHH307 pKa = 6.7SDD309 pKa = 3.15DD310 pKa = 4.05ALATGLYY317 pKa = 10.82ANFRR321 pKa = 11.84FANSDD326 pKa = 3.1TGVAVTGNAGDD337 pKa = 4.05VIPP340 pKa = 5.25

Molecular weight: 38.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

907

124

340

226.8

25.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.056 ± 0.65

1.433 ± 0.804

4.851 ± 0.57

4.851 ± 1.526

3.087 ± 0.642

5.182 ± 0.612

2.646 ± 0.123

4.631 ± 0.569

6.615 ± 1.351

8.049 ± 1.545

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.544 ± 0.326

4.41 ± 1.236

5.513 ± 1.152

3.638 ± 0.199

8.049 ± 1.082

9.372 ± 1.339

7.718 ± 1.409

6.505 ± 0.62

1.654 ± 0.938

3.197 ± 0.896
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski