Amino acid dipepetide frequency for Maylandia zebra (zebra mbuna)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.914AlaAla: 5.914 ± 0.024	1.274AlaCys: 1.274 ± 0.009	3.12AlaAsp: 3.12 ± 0.016	4.522AlaGlu: 4.522 ± 0.023	2.404AlaPhe: 2.404 ± 0.012	4.158AlaGly: 4.158 ± 0.018	1.475AlaHis: 1.475 ± 0.01	2.755AlaIle: 2.755 ± 0.015	3.369AlaLys: 3.369 ± 0.017	6.37AlaLeu: 6.37 ± 0.023	1.524AlaMet: 1.524 ± 0.009	2.184AlaAsn: 2.184 ± 0.012	3.314AlaPro: 3.314 ± 0.02	2.829AlaGln: 2.829 ± 0.015	2.993AlaArg: 2.993 ± 0.015	5.401AlaSer: 5.401 ± 0.021	3.487AlaThr: 3.487 ± 0.014	4.9AlaVal: 4.9 ± 0.02	0.647AlaTrp: 0.647 ± 0.007	1.528AlaTyr: 1.528 ± 0.009	0.0AlaXaa: 0.0 ± 0.0
Cys	1.224CysAla: 1.224 ± 0.009	0.69CysCys: 0.69 ± 0.008	1.185CysAsp: 1.185 ± 0.013	1.282CysGlu: 1.282 ± 0.012	0.967CysPhe: 0.967 ± 0.008	1.675CysGly: 1.675 ± 0.016	0.681CysHis: 0.681 ± 0.007	1.045CysIle: 1.045 ± 0.009	1.209CysLys: 1.209 ± 0.01	2.235CysLeu: 2.235 ± 0.015	0.485CysMet: 0.485 ± 0.006	0.889CysAsn: 0.889 ± 0.008	1.294CysPro: 1.294 ± 0.013	1.055CysGln: 1.055 ± 0.01	1.292CysArg: 1.292 ± 0.01	2.229CysSer: 2.229 ± 0.016	1.256CysThr: 1.256 ± 0.009	1.614CysVal: 1.614 ± 0.013	0.31CysTrp: 0.31 ± 0.004	0.669CysTyr: 0.669 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	2.902AspAla: 2.902 ± 0.014	1.164AspCys: 1.164 ± 0.01	3.037AspAsp: 3.037 ± 0.019	3.681AspGlu: 3.681 ± 0.021	2.152AspPhe: 2.152 ± 0.012	3.572AspGly: 3.572 ± 0.021	1.194AspHis: 1.194 ± 0.009	2.751AspIle: 2.751 ± 0.024	2.718AspLys: 2.718 ± 0.014	4.941AspLeu: 4.941 ± 0.018	1.259AspMet: 1.259 ± 0.009	1.958AspAsn: 1.958 ± 0.012	2.762AspPro: 2.762 ± 0.014	1.92AspGln: 1.92 ± 0.01	2.689AspArg: 2.689 ± 0.016	4.452AspSer: 4.452 ± 0.02	2.674AspThr: 2.674 ± 0.011	3.285AspVal: 3.285 ± 0.022	0.68AspTrp: 0.68 ± 0.006	1.563AspTyr: 1.563 ± 0.009	0.0AspXaa: 0.0 ± 0.0
Glu	4.607GluAla: 4.607 ± 0.024	1.229GluCys: 1.229 ± 0.012	4.256GluAsp: 4.256 ± 0.021	7.382GluGlu: 7.382 ± 0.045	1.927GluPhe: 1.927 ± 0.01	4.016GluGly: 4.016 ± 0.022	1.442GluHis: 1.442 ± 0.009	2.936GluIle: 2.936 ± 0.013	4.813GluLys: 4.813 ± 0.027	6.045GluLeu: 6.045 ± 0.025	1.719GluMet: 1.719 ± 0.01	2.814GluAsn: 2.814 ± 0.014	2.675GluPro: 2.675 ± 0.018	3.021GluGln: 3.021 ± 0.016	4.128GluArg: 4.128 ± 0.02	4.375GluSer: 4.375 ± 0.021	3.45GluThr: 3.45 ± 0.016	4.263GluVal: 4.263 ± 0.02	0.712GluTrp: 0.712 ± 0.007	1.587GluTyr: 1.587 ± 0.009	0.0GluXaa: 0.0 ± 0.0
Phe	1.932PheAla: 1.932 ± 0.011	1.04PheCys: 1.04 ± 0.008	1.784PheAsp: 1.784 ± 0.01	1.842PheGlu: 1.842 ± 0.011	1.744PhePhe: 1.744 ± 0.013	2.22PheGly: 2.22 ± 0.014	1.061PheHis: 1.061 ± 0.008	2.101PheIle: 2.101 ± 0.013	1.927PheLys: 1.927 ± 0.012	4.033PheLeu: 4.033 ± 0.019	0.838PheMet: 0.838 ± 0.008	1.564PheAsn: 1.564 ± 0.008	1.852PhePro: 1.852 ± 0.01	1.633PheGln: 1.633 ± 0.01	1.862PheArg: 1.862 ± 0.01	3.536PheSer: 3.536 ± 0.017	2.452PheThr: 2.452 ± 0.014	2.306PheVal: 2.306 ± 0.012	0.5PheTrp: 0.5 ± 0.005	1.276PheTyr: 1.276 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	3.8GlyAla: 3.8 ± 0.017	1.269GlyCys: 1.269 ± 0.01	3.147GlyAsp: 3.147 ± 0.014	4.055GlyGlu: 4.055 ± 0.02	2.447GlyPhe: 2.447 ± 0.015	5.067GlyGly: 5.067 ± 0.03	1.612GlyHis: 1.612 ± 0.01	2.664GlyIle: 2.664 ± 0.014	3.664GlyLys: 3.664 ± 0.022	5.388GlyLeu: 5.388 ± 0.019	1.429GlyMet: 1.429 ± 0.011	2.553GlyAsn: 2.553 ± 0.011	3.105GlyPro: 3.105 ± 0.035	2.67GlyGln: 2.67 ± 0.016	3.522GlyArg: 3.522 ± 0.018	5.627GlySer: 5.627 ± 0.027	3.409GlyThr: 3.409 ± 0.015	3.977GlyVal: 3.977 ± 0.017	0.774GlyTrp: 0.774 ± 0.007	1.839GlyTyr: 1.839 ± 0.013	0.0GlyXaa: 0.0 ± 0.0
His	1.325HisAla: 1.325 ± 0.009	0.804HisCys: 0.804 ± 0.009	0.974HisAsp: 0.974 ± 0.008	1.211HisGlu: 1.211 ± 0.008	1.104HisPhe: 1.104 ± 0.008	1.499HisGly: 1.499 ± 0.01	1.072HisHis: 1.072 ± 0.012	1.371HisIle: 1.371 ± 0.009	1.333HisLys: 1.333 ± 0.011	2.753HisLeu: 2.753 ± 0.014	0.703HisMet: 0.703 ± 0.008	1.062HisAsn: 1.062 ± 0.008	1.584HisPro: 1.584 ± 0.011	1.277HisGln: 1.277 ± 0.01	1.617HisArg: 1.617 ± 0.011	2.516HisSer: 2.516 ± 0.014	1.694HisThr: 1.694 ± 0.014	1.466HisVal: 1.466 ± 0.008	0.343HisTrp: 0.343 ± 0.004	0.875HisTyr: 0.875 ± 0.008	0.0HisXaa: 0.0 ± 0.0
Ile	2.541IleAla: 2.541 ± 0.014	1.18IleCys: 1.18 ± 0.01	2.159IleAsp: 2.159 ± 0.013	2.41IleGlu: 2.41 ± 0.014	1.959IlePhe: 1.959 ± 0.013	2.338IleGly: 2.338 ± 0.012	1.371IleHis: 1.371 ± 0.01	2.595IleIle: 2.595 ± 0.014	2.695IleLys: 2.695 ± 0.015	4.473IleLeu: 4.473 ± 0.019	1.109IleMet: 1.109 ± 0.008	2.087IleAsn: 2.087 ± 0.011	2.524IlePro: 2.524 ± 0.012	2.28IleGln: 2.28 ± 0.012	2.443IleArg: 2.443 ± 0.011	3.964IleSer: 3.964 ± 0.018	2.95IleThr: 2.95 ± 0.015	2.662IleVal: 2.662 ± 0.015	0.506IleTrp: 0.506 ± 0.006	1.499IleTyr: 1.499 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	3.77LysAla: 3.77 ± 0.018	1.099LysCys: 1.099 ± 0.009	3.267LysAsp: 3.267 ± 0.018	4.764LysGlu: 4.764 ± 0.026	1.676LysPhe: 1.676 ± 0.013	3.239LysGly: 3.239 ± 0.024	1.479LysHis: 1.479 ± 0.009	2.62LysIle: 2.62 ± 0.015	4.609LysLys: 4.609 ± 0.027	5.095LysLeu: 5.095 ± 0.019	1.522LysMet: 1.522 ± 0.012	2.417LysAsn: 2.417 ± 0.013	2.979LysPro: 2.979 ± 0.017	2.592LysGln: 2.592 ± 0.015	3.403LysArg: 3.403 ± 0.016	3.961LysSer: 3.961 ± 0.018	3.389LysThr: 3.389 ± 0.015	3.606LysVal: 3.606 ± 0.016	0.59LysTrp: 0.59 ± 0.006	1.578LysTyr: 1.578 ± 0.01	0.0LysXaa: 0.0 ± 0.0
Leu	5.892LeuAla: 5.892 ± 0.023	2.341LeuCys: 2.341 ± 0.013	4.798LeuAsp: 4.798 ± 0.02	6.304LeuGlu: 6.304 ± 0.028	3.561LeuPhe: 3.561 ± 0.017	5.054LeuGly: 5.054 ± 0.019	2.723LeuHis: 2.723 ± 0.014	4.018LeuIle: 4.018 ± 0.019	5.808LeuLys: 5.808 ± 0.022	9.987LeuLeu: 9.987 ± 0.041	2.098LeuMet: 2.098 ± 0.011	3.732LeuAsn: 3.732 ± 0.013	5.283LeuPro: 5.283 ± 0.023	5.379LeuGln: 5.379 ± 0.022	5.471LeuArg: 5.471 ± 0.022	8.439LeuSer: 8.439 ± 0.027	5.434LeuThr: 5.434 ± 0.018	5.459LeuVal: 5.459 ± 0.02	1.075LeuTrp: 1.075 ± 0.008	2.67LeuTyr: 2.67 ± 0.013	0.0LeuXaa: 0.0 ± 0.0
Met	1.857MetAla: 1.857 ± 0.011	0.508MetCys: 0.508 ± 0.005	1.366MetAsp: 1.366 ± 0.01	1.915MetGlu: 1.915 ± 0.012	0.887MetPhe: 0.887 ± 0.008	1.359MetGly: 1.359 ± 0.009	0.496MetHis: 0.496 ± 0.005	0.928MetIle: 0.928 ± 0.007	1.526MetLys: 1.526 ± 0.011	2.087MetLeu: 2.087 ± 0.011	0.697MetMet: 0.697 ± 0.007	0.942MetAsn: 0.942 ± 0.008	1.085MetPro: 1.085 ± 0.014	0.976MetGln: 0.976 ± 0.007	1.172MetArg: 1.172 ± 0.009	1.869MetSer: 1.869 ± 0.01	1.301MetThr: 1.301 ± 0.008	1.529MetVal: 1.529 ± 0.01	0.27MetTrp: 0.27 ± 0.004	0.651MetTyr: 0.651 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.17AsnAla: 2.17 ± 0.012	0.917AsnCys: 0.917 ± 0.008	1.781AsnAsp: 1.781 ± 0.012	2.14AsnGlu: 2.14 ± 0.012	1.487AsnPhe: 1.487 ± 0.01	2.788AsnGly: 2.788 ± 0.014	1.016AsnHis: 1.016 ± 0.007	2.285AsnIle: 2.285 ± 0.012	2.331AsnLys: 2.331 ± 0.012	3.766AsnLeu: 3.766 ± 0.015	1.055AsnMet: 1.055 ± 0.008	1.946AsnAsn: 1.946 ± 0.011	2.256AsnPro: 2.256 ± 0.011	1.794AsnGln: 1.794 ± 0.01	2.001AsnArg: 2.001 ± 0.01	3.371AsnSer: 3.371 ± 0.016	2.407AsnThr: 2.407 ± 0.013	2.35AsnVal: 2.35 ± 0.012	0.457AsnTrp: 0.457 ± 0.005	1.194AsnTyr: 1.194 ± 0.009	0.0AsnXaa: 0.0 ± 0.0
Pro	3.974ProAla: 3.974 ± 0.019	1.069ProCys: 1.069 ± 0.011	2.813ProAsp: 2.813 ± 0.016	3.661ProGlu: 3.661 ± 0.018	1.792ProPhe: 1.792 ± 0.01	3.876ProGly: 3.876 ± 0.03	1.495ProHis: 1.495 ± 0.012	1.917ProIle: 1.917 ± 0.01	2.575ProLys: 2.575 ± 0.026	4.75ProLeu: 4.75 ± 0.02	0.991ProMet: 0.991 ± 0.009	1.939ProAsn: 1.939 ± 0.012	5.107ProPro: 5.107 ± 0.035	2.611ProGln: 2.611 ± 0.016	2.645ProArg: 2.645 ± 0.015	5.491ProSer: 5.491 ± 0.032	3.064ProThr: 3.064 ± 0.016	3.721ProVal: 3.721 ± 0.019	0.549ProTrp: 0.549 ± 0.006	1.462ProTyr: 1.462 ± 0.011	0.0ProXaa: 0.0 ± 0.0
Gln	3.076GlnAla: 3.076 ± 0.016	1.013GlnCys: 1.013 ± 0.011	2.231GlnAsp: 2.231 ± 0.011	3.373GlnGlu: 3.373 ± 0.016	1.362GlnPhe: 1.362 ± 0.009	2.625GlnGly: 2.625 ± 0.015	1.379GlnHis: 1.379 ± 0.009	1.995GlnIle: 1.995 ± 0.011	2.671GlnLys: 2.671 ± 0.013	4.483GlnLeu: 4.483 ± 0.019	1.151GlnMet: 1.151 ± 0.008	1.809GlnAsn: 1.809 ± 0.011	2.525GlnPro: 2.525 ± 0.017	3.262GlnGln: 3.262 ± 0.031	3.001GlnArg: 3.001 ± 0.015	3.525GlnSer: 3.525 ± 0.014	2.615GlnThr: 2.615 ± 0.013	2.764GlnVal: 2.764 ± 0.012	0.553GlnTrp: 0.553 ± 0.005	1.259GlnTyr: 1.259 ± 0.008	0.0GlnXaa: 0.0 ± 0.0
Arg	3.314ArgAla: 3.314 ± 0.014	1.257ArgCys: 1.257 ± 0.012	2.853ArgAsp: 2.853 ± 0.015	3.777ArgGlu: 3.777 ± 0.02	1.984ArgPhe: 1.984 ± 0.011	3.328ArgGly: 3.328 ± 0.02	1.554ArgHis: 1.554 ± 0.011	2.41ArgIle: 2.41 ± 0.012	3.54ArgLys: 3.54 ± 0.015	5.172ArgLeu: 5.172 ± 0.018	1.256ArgMet: 1.256 ± 0.009	2.13ArgAsn: 2.13 ± 0.011	2.821ArgPro: 2.821 ± 0.014	2.586ArgGln: 2.586 ± 0.014	4.143ArgArg: 4.143 ± 0.021	4.435ArgSer: 4.435 ± 0.022	2.854ArgThr: 2.854 ± 0.014	3.277ArgVal: 3.277 ± 0.014	0.669ArgTrp: 0.669 ± 0.006	1.574ArgTyr: 1.574 ± 0.01	0.0ArgXaa: 0.0 ± 0.0
Ser	5.616SerAla: 5.616 ± 0.02	2.128SerCys: 2.128 ± 0.013	4.402SerAsp: 4.402 ± 0.019	4.946SerGlu: 4.946 ± 0.024	3.272SerPhe: 3.272 ± 0.016	5.586SerGly: 5.586 ± 0.021	2.299SerHis: 2.299 ± 0.013	3.485SerIle: 3.485 ± 0.015	4.08SerLys: 4.08 ± 0.019	8.43SerLeu: 8.43 ± 0.033	1.795SerMet: 1.795 ± 0.01	3.112SerAsn: 3.112 ± 0.013	5.77SerPro: 5.77 ± 0.037	3.917SerGln: 3.917 ± 0.017	4.403SerArg: 4.403 ± 0.022	10.328SerSer: 10.328 ± 0.049	4.966SerThr: 4.966 ± 0.024	5.582SerVal: 5.582 ± 0.02	1.033SerTrp: 1.033 ± 0.009	2.24SerTyr: 2.24 ± 0.011	0.0SerXaa: 0.0 ± 0.0
Thr	4.104ThrAla: 4.104 ± 0.015	1.473ThrCys: 1.473 ± 0.015	2.953ThrAsp: 2.953 ± 0.014	3.808ThrGlu: 3.808 ± 0.02	2.231ThrPhe: 2.231 ± 0.011	3.838ThrGly: 3.838 ± 0.018	1.5ThrHis: 1.5 ± 0.011	2.541ThrIle: 2.541 ± 0.014	2.793ThrLys: 2.793 ± 0.014	5.502ThrLeu: 5.502 ± 0.02	1.228ThrMet: 1.228 ± 0.008	2.075ThrAsn: 2.075 ± 0.009	3.605ThrPro: 3.605 ± 0.02	2.398ThrGln: 2.398 ± 0.012	2.49ThrArg: 2.49 ± 0.013	4.962ThrSer: 4.962 ± 0.024	3.486ThrThr: 3.486 ± 0.028	4.33ThrVal: 4.33 ± 0.017	0.687ThrTrp: 0.687 ± 0.007	1.506ThrTyr: 1.506 ± 0.009	0.0ThrXaa: 0.0 ± 0.0
Val	4.058ValAla: 4.058 ± 0.016	1.794ValCys: 1.794 ± 0.013	3.185ValAsp: 3.185 ± 0.02	3.992ValGlu: 3.992 ± 0.017	2.796ValPhe: 2.796 ± 0.014	3.473ValGly: 3.473 ± 0.017	1.634ValHis: 1.634 ± 0.009	3.141ValIle: 3.141 ± 0.015	3.712ValLys: 3.712 ± 0.016	6.135ValLeu: 6.135 ± 0.024	1.542ValMet: 1.542 ± 0.009	2.503ValAsn: 2.503 ± 0.013	3.183ValPro: 3.183 ± 0.016	2.748ValGln: 2.748 ± 0.011	3.225ValArg: 3.225 ± 0.015	5.453ValSer: 5.453 ± 0.019	4.176ValThr: 4.176 ± 0.021	4.395ValVal: 4.395 ± 0.019	0.783ValTrp: 0.783 ± 0.007	1.872ValTyr: 1.872 ± 0.01	0.0ValXaa: 0.0 ± 0.0
Trp	0.66TrpAla: 0.66 ± 0.007	0.259TrpCys: 0.259 ± 0.004	0.645TrpAsp: 0.645 ± 0.006	0.744TrpGlu: 0.744 ± 0.007	0.47TrpPhe: 0.47 ± 0.006	0.638TrpGly: 0.638 ± 0.007	0.272TrpHis: 0.272 ± 0.004	0.623TrpIle: 0.623 ± 0.006	0.737TrpLys: 0.737 ± 0.006	1.19TrpLeu: 1.19 ± 0.009	0.344TrpMet: 0.344 ± 0.004	0.531TrpAsn: 0.531 ± 0.006	0.434TrpPro: 0.434 ± 0.005	0.476TrpGln: 0.476 ± 0.005	0.739TrpArg: 0.739 ± 0.007	0.983TrpSer: 0.983 ± 0.009	0.718TrpThr: 0.718 ± 0.007	0.704TrpVal: 0.704 ± 0.007	0.198TrpTrp: 0.198 ± 0.003	0.375TrpTyr: 0.375 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.431TyrAla: 1.431 ± 0.008	0.78TyrCys: 0.78 ± 0.007	1.397TyrAsp: 1.397 ± 0.011	1.588TyrGlu: 1.588 ± 0.009	1.279TyrPhe: 1.279 ± 0.009	1.67TyrGly: 1.67 ± 0.009	0.829TyrHis: 0.829 ± 0.007	1.562TyrIle: 1.562 ± 0.01	1.544TyrLys: 1.544 ± 0.011	2.687TyrLeu: 2.687 ± 0.014	0.672TyrMet: 0.672 ± 0.007	1.264TyrAsn: 1.264 ± 0.008	1.346TyrPro: 1.346 ± 0.009	1.248TyrGln: 1.248 ± 0.008	1.696TyrArg: 1.696 ± 0.009	2.475TyrSer: 2.475 ± 0.013	1.729TyrThr: 1.729 ± 0.01	1.613TyrVal: 1.613 ± 0.01	0.406TyrTrp: 0.406 ± 0.006	1.044TyrTyr: 1.044 ± 0.009	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.011XaaXaa: 0.011 ± 0.004

Statistics based on 37295 proteins (20228919 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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