Roseobacter virus SIO1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Cobavirinae; Siovirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9G0H9|Q9G0H9_9CAUD Gp6 OS=Roseobacter virus SIO1 OX=136084 PE=4 SV=1
MM1 pKa = 7.59AKK3 pKa = 10.07KK4 pKa = 8.81PTVTTLQSGFNSTEE18 pKa = 3.71VLNEE22 pKa = 3.53NFEE25 pKa = 4.63NIRR28 pKa = 11.84DD29 pKa = 3.92AFDD32 pKa = 3.28NTLSLDD38 pKa = 3.47GSTPNAMQADD48 pKa = 4.36LDD50 pKa = 4.5LNGNNLIGATGLLINGTDD68 pKa = 3.54YY69 pKa = 11.46LADD72 pKa = 3.58VEE74 pKa = 4.35AAKK77 pKa = 10.42AAALVAQAAAEE88 pKa = 3.93LAEE91 pKa = 4.56NNAEE95 pKa = 4.13TAEE98 pKa = 4.25VNAEE102 pKa = 3.92ASEE105 pKa = 4.27TAAGLSATAAATSATNAGASEE126 pKa = 4.25TAASASATASATSATNSATSASQAATSASAAAVSEE161 pKa = 4.53GNAATSEE168 pKa = 4.27TNAANSATSASGSASTATTQASAASVSATNAATSASNAATSATNAAASQAAAATSEE224 pKa = 4.33TNAAASEE231 pKa = 4.56STVTTSATNAATSEE245 pKa = 4.17ANAATSASTATTQATNAATSASTASTSATNAATSEE280 pKa = 4.26TNAASSASSAASSATSAQASKK301 pKa = 10.94DD302 pKa = 3.5AALAALDD309 pKa = 4.23SFDD312 pKa = 5.84DD313 pKa = 4.2RR314 pKa = 11.84YY315 pKa = 11.02LGQKK319 pKa = 10.02VSDD322 pKa = 3.76PTLDD326 pKa = 3.37NDD328 pKa = 4.41GNALVAGALYY338 pKa = 10.7FNTTDD343 pKa = 4.63GIMKK347 pKa = 10.1VYY349 pKa = 10.58DD350 pKa = 3.62GSVWLAAYY358 pKa = 9.86ASLSGALIATNNLSDD373 pKa = 4.02VLDD376 pKa = 3.9VTASRR381 pKa = 11.84TNLGLGTAATTASTDD396 pKa = 3.38YY397 pKa = 10.08ATAAQGALAVSAVQPNDD414 pKa = 3.4SPSFGSVTVTGTVDD428 pKa = 3.38GRR430 pKa = 11.84NVAADD435 pKa = 3.79GSKK438 pKa = 10.81LDD440 pKa = 4.7GIEE443 pKa = 4.38AGATADD449 pKa = 3.53QTASEE454 pKa = 4.59ILTSIKK460 pKa = 9.85TVDD463 pKa = 3.58GSGSGLDD470 pKa = 3.96ADD472 pKa = 4.95LLDD475 pKa = 5.53GIHH478 pKa = 6.98ASSFLQGNQTITLSGDD494 pKa = 3.19VSGSGTTSILVTVADD509 pKa = 4.96DD510 pKa = 3.67SHH512 pKa = 7.71NHH514 pKa = 6.49IIANVDD520 pKa = 3.53GLQTALDD527 pKa = 4.28GKK529 pKa = 8.65WSKK532 pKa = 10.91GADD535 pKa = 2.97IGGGCC540 pKa = 4.62

Molecular weight: 51.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9G0G0|Q9G0G0_9CAUD Gp26 OS=Roseobacter virus SIO1 OX=136084 PE=4 SV=1
MM1 pKa = 6.89VAVTGTVLAIAGTTAGIVGTVKK23 pKa = 10.38AGKK26 pKa = 9.39AARR29 pKa = 11.84RR30 pKa = 11.84SARR33 pKa = 11.84AQQQAQEE40 pKa = 4.3VQAKK44 pKa = 7.43RR45 pKa = 11.84QRR47 pKa = 11.84RR48 pKa = 11.84AAIRR52 pKa = 11.84SNILASARR60 pKa = 11.84AKK62 pKa = 10.75ASAQAAGTSQSSGLSGAIGAGRR84 pKa = 11.84SQLGAEE90 pKa = 4.6LGFGSQLSGLSANISKK106 pKa = 10.58FDD108 pKa = 3.76MQAQTYY114 pKa = 10.64GDD116 pKa = 3.44IAKK119 pKa = 10.27LGFSAAGNAPTIGGYY134 pKa = 10.56AEE136 pKa = 4.31DD137 pKa = 4.6LYY139 pKa = 11.68GFFKK143 pKa = 10.87KK144 pKa = 10.54

Molecular weight: 14.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

9898

77

1484

309.3

34.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.547 ± 0.936

0.808 ± 0.205

6.486 ± 0.261

6.466 ± 0.35

3.91 ± 0.247

7.577 ± 0.318

1.475 ± 0.208

4.607 ± 0.304

6.274 ± 0.381

7.961 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.187

4.86 ± 0.284

3.081 ± 0.195

3.566 ± 0.22

4.799 ± 0.32

7.577 ± 0.543

6.86 ± 0.45

6.951 ± 0.323

1.273 ± 0.182

3.637 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski