Rhizobium sp. Leaf371

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4411 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5WSJ8|A0A0Q5WSJ8_9RHIZ ABC transporter ATP-binding protein OS=Rhizobium sp. Leaf371 OX=1736355 GN=ASG39_20835 PE=4 SV=1
MM1 pKa = 7.5IANTGNPLIDD11 pKa = 4.14GLLSGNAWNAATLTYY26 pKa = 10.66AFPTTTSAYY35 pKa = 8.41TYY37 pKa = 10.29TSGTYY42 pKa = 9.77RR43 pKa = 11.84DD44 pKa = 3.78VAPVSLAQQKK54 pKa = 10.11AALFFLEE61 pKa = 4.45QSSGPAANDD70 pKa = 3.56GFSVEE75 pKa = 4.96GFTLLKK81 pKa = 10.06FAAGSATNATLRR93 pKa = 11.84FAEE96 pKa = 4.93SPTADD101 pKa = 3.23PTAYY105 pKa = 10.37AFYY108 pKa = 10.14PGNYY112 pKa = 9.49DD113 pKa = 3.06SAGDD117 pKa = 3.57VWFGTAYY124 pKa = 10.52AGTEE128 pKa = 3.65YY129 pKa = 10.51DD130 pKa = 3.62YY131 pKa = 11.18RR132 pKa = 11.84SPKK135 pKa = 9.36IGTYY139 pKa = 9.13AWHH142 pKa = 6.27TMGHH146 pKa = 6.52EE147 pKa = 4.12IGHH150 pKa = 6.44ALGLKK155 pKa = 9.81HH156 pKa = 5.8GHH158 pKa = 6.35EE159 pKa = 4.67DD160 pKa = 3.13AALPSNVDD168 pKa = 3.4SVEE171 pKa = 3.88YY172 pKa = 10.86SIMTYY177 pKa = 9.9RR178 pKa = 11.84AYY180 pKa = 9.74IGAPLTGYY188 pKa = 10.65SYY190 pKa = 11.62GLNDD194 pKa = 3.76APQSFMSLDD203 pKa = 3.34IAALQAMYY211 pKa = 10.4GADD214 pKa = 3.49YY215 pKa = 10.65TVMNGNTVYY224 pKa = 10.67RR225 pKa = 11.84WTPDD229 pKa = 2.78SGQTIIDD236 pKa = 3.84GVVTIAPGANRR247 pKa = 11.84IFATIWDD254 pKa = 4.11GGGTDD259 pKa = 3.77TFDD262 pKa = 3.19LSAYY266 pKa = 7.55KK267 pKa = 9.52TALSIDD273 pKa = 4.13LQPGKK278 pKa = 10.88YY279 pKa = 9.88SLFSTGQTADD289 pKa = 3.64LGGGPNNGHH298 pKa = 6.24ARR300 pKa = 11.84GNIFNALLYY309 pKa = 10.7KK310 pKa = 10.9NNTASLIEE318 pKa = 4.04NVLGGSGNDD327 pKa = 3.14RR328 pKa = 11.84IVGNQVQNKK337 pKa = 9.57LFGNAGNDD345 pKa = 3.33KK346 pKa = 11.0LFGLTGNDD354 pKa = 2.97ILVGGIGADD363 pKa = 3.53TLDD366 pKa = 4.42GGSGTDD372 pKa = 3.1TASYY376 pKa = 10.32EE377 pKa = 4.03DD378 pKa = 3.78AKK380 pKa = 11.33AGVLANLTQSSLNTGIAAGDD400 pKa = 3.73VYY402 pKa = 11.56VSIEE406 pKa = 4.06NLTGSAYY413 pKa = 10.36ADD415 pKa = 3.67SLIGDD420 pKa = 4.31AAANVLKK427 pKa = 10.83GGAGNDD433 pKa = 3.73TLSGGGGSDD442 pKa = 3.49VLIGGIGADD451 pKa = 3.31RR452 pKa = 11.84LNGNGGSDD460 pKa = 3.16TASYY464 pKa = 11.04EE465 pKa = 4.03DD466 pKa = 3.54ATAGVTANLLNASRR480 pKa = 11.84NTGFAAGDD488 pKa = 3.25IYY490 pKa = 11.31GSIEE494 pKa = 4.36HH495 pKa = 6.26LTGSAFDD502 pKa = 5.25DD503 pKa = 4.0LLAGNASANTLRR515 pKa = 11.84GGAGRR520 pKa = 11.84DD521 pKa = 3.63VLSGDD526 pKa = 3.91SGNDD530 pKa = 3.41TLIGGAGADD539 pKa = 3.81KK540 pKa = 10.86LYY542 pKa = 11.25GGAGTDD548 pKa = 3.33LASYY552 pKa = 10.38EE553 pKa = 4.2DD554 pKa = 3.71ATAGVIANLGKK565 pKa = 10.61ASANTGFAAGDD576 pKa = 3.77TYY578 pKa = 11.85ASISGLIGSAFDD590 pKa = 4.05DD591 pKa = 4.09QLTGDD596 pKa = 3.8TGANILIGGNGRR608 pKa = 11.84DD609 pKa = 3.83SLNGGNGNDD618 pKa = 3.79RR619 pKa = 11.84LIGGAGLDD627 pKa = 3.98TLTGGAGADD636 pKa = 3.51TFVYY640 pKa = 10.37AATQEE645 pKa = 4.43SGPSSSARR653 pKa = 11.84DD654 pKa = 3.95LITDD658 pKa = 4.02FSGTSGDD665 pKa = 4.12RR666 pKa = 11.84IDD668 pKa = 4.57VSGIDD673 pKa = 3.55SNLVIGGVQHH683 pKa = 6.63FSLIGGAAFSNTAGQLRR700 pKa = 11.84YY701 pKa = 8.78VTLSTSSFVYY711 pKa = 10.81GDD713 pKa = 3.55TNGDD717 pKa = 3.16GTADD721 pKa = 3.36FTIAFDD727 pKa = 3.91DD728 pKa = 4.05AVTFRR733 pKa = 11.84SDD735 pKa = 3.3YY736 pKa = 11.08FIVV739 pKa = 3.42

Molecular weight: 74.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5WAD6|A0A0Q5WAD6_9RHIZ Pyridoxamine 5'-phosphate oxidase OS=Rhizobium sp. Leaf371 OX=1736355 GN=ASG39_08540 PE=4 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.11RR4 pKa = 11.84TYY6 pKa = 10.36QPSKK10 pKa = 9.73LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.64GFRR20 pKa = 11.84ARR22 pKa = 11.84IATKK26 pKa = 9.98GGRR29 pKa = 11.84KK30 pKa = 9.73VIVARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.03RR42 pKa = 11.84LSAA45 pKa = 4.03

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4411

0

4411

1421238

29

3007

322.2

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.398 ± 0.054

0.746 ± 0.011

5.937 ± 0.032

5.518 ± 0.033

3.826 ± 0.025

8.42 ± 0.036

1.976 ± 0.018

5.587 ± 0.029

3.349 ± 0.036

10.047 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.018

2.6 ± 0.021

4.949 ± 0.026

3.021 ± 0.026

6.894 ± 0.04

5.672 ± 0.027

5.73 ± 0.027

7.422 ± 0.029

1.198 ± 0.014

2.178 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski