Sathuvachari virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; unclassified Orbivirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1H1Z8|M1H1Z8_9REOV Outer capsid protein VP5 OS=Sathuvachari virus OX=1289939 PE=3 SV=1
MM1 pKa = 7.37TRR3 pKa = 11.84LTLLAPGDD11 pKa = 4.07VIRR14 pKa = 11.84KK15 pKa = 9.61AEE17 pKa = 3.98QEE19 pKa = 4.01LKK21 pKa = 10.7ARR23 pKa = 11.84RR24 pKa = 11.84IDD26 pKa = 3.87VLIQNDD32 pKa = 3.65AEE34 pKa = 4.34EE35 pKa = 4.73GGKK38 pKa = 8.31EE39 pKa = 3.97QEE41 pKa = 4.13RR42 pKa = 11.84KK43 pKa = 10.36EE44 pKa = 3.82EE45 pKa = 4.24DD46 pKa = 3.16EE47 pKa = 4.75AGGKK51 pKa = 9.09RR52 pKa = 11.84VQEE55 pKa = 4.14STNEE59 pKa = 3.82RR60 pKa = 11.84KK61 pKa = 8.13EE62 pKa = 4.16TGNATEE68 pKa = 4.66RR69 pKa = 11.84GGEE72 pKa = 4.08GTQKK76 pKa = 10.62NDD78 pKa = 2.86EE79 pKa = 4.91DD80 pKa = 3.78GGEE83 pKa = 4.1RR84 pKa = 11.84QGRR87 pKa = 11.84EE88 pKa = 3.83AEE90 pKa = 3.92EE91 pKa = 4.1VQIHH95 pKa = 6.16KK96 pKa = 10.7AFSEE100 pKa = 4.19NKK102 pKa = 9.85GAGDD106 pKa = 4.32RR107 pKa = 11.84DD108 pKa = 4.24DD109 pKa = 5.04EE110 pKa = 4.51VQATKK115 pKa = 9.69QEE117 pKa = 4.12KK118 pKa = 9.94EE119 pKa = 4.12DD120 pKa = 3.75MVVLDD125 pKa = 4.61SEE127 pKa = 4.57VQKK130 pKa = 11.37VIDD133 pKa = 3.63KK134 pKa = 10.9LYY136 pKa = 10.69GIRR139 pKa = 11.84LQTIDD144 pKa = 4.41TITEE148 pKa = 3.54IGDD151 pKa = 3.68RR152 pKa = 11.84VILKK156 pKa = 10.36LGSNVLKK163 pKa = 10.69QIGIGKK169 pKa = 9.8EE170 pKa = 3.94KK171 pKa = 10.8VQEE174 pKa = 4.06QNDD177 pKa = 3.36ALTRR181 pKa = 11.84FKK183 pKa = 10.06KK184 pKa = 9.23TKK186 pKa = 7.27TKK188 pKa = 9.9KK189 pKa = 9.06GRR191 pKa = 11.84VMEE194 pKa = 4.2VQFIEE199 pKa = 4.31SLKK202 pKa = 10.69GLEE205 pKa = 4.33AEE207 pKa = 4.51IGVDD211 pKa = 3.3KK212 pKa = 11.12NRR214 pKa = 11.84EE215 pKa = 3.36VDD217 pKa = 3.55TRR219 pKa = 11.84VSQSGVVLVTNDD231 pKa = 2.95RR232 pKa = 11.84RR233 pKa = 11.84YY234 pKa = 9.89VSQAHH239 pKa = 6.47VIFTCPTGDD248 pKa = 3.8TTWKK252 pKa = 7.91EE253 pKa = 4.07TARR256 pKa = 11.84QATKK260 pKa = 10.46RR261 pKa = 11.84SDD263 pKa = 2.65IRR265 pKa = 11.84AYY267 pKa = 10.8VHH269 pKa = 7.26DD270 pKa = 4.66PANGDD275 pKa = 3.72SEE277 pKa = 4.76KK278 pKa = 11.24GLLALVDD285 pKa = 4.25LLL287 pKa = 4.74

Molecular weight: 32.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1H204|M1H204_9REOV Non-structural protein NS3 OS=Sathuvachari virus OX=1289939 PE=3 SV=1
MM1 pKa = 7.26MLTAVSHH8 pKa = 5.47IHH10 pKa = 6.14SGGSRR15 pKa = 11.84EE16 pKa = 4.18TRR18 pKa = 11.84DD19 pKa = 3.12MTAPMLPVSDD29 pKa = 5.25KK30 pKa = 9.69EE31 pKa = 4.26TSLNILTNALTSGTGSTAQQKK52 pKa = 9.24NEE54 pKa = 3.9KK55 pKa = 9.87ASYY58 pKa = 9.85GAIAEE63 pKa = 4.26ALRR66 pKa = 11.84DD67 pKa = 3.93EE68 pKa = 4.58PAVRR72 pKa = 11.84YY73 pKa = 9.34AKK75 pKa = 10.93YY76 pKa = 9.57EE77 pKa = 4.09VNSRR81 pKa = 11.84SIPKK85 pKa = 9.52VEE87 pKa = 3.94SRR89 pKa = 11.84IKK91 pKa = 10.39RR92 pKa = 11.84LRR94 pKa = 11.84RR95 pKa = 11.84TKK97 pKa = 10.26KK98 pKa = 9.13LYY100 pKa = 10.66KK101 pKa = 9.82IFEE104 pKa = 4.52VVFGVATLLLGILTTSMAEE123 pKa = 3.61IEE125 pKa = 4.19EE126 pKa = 4.48WKK128 pKa = 10.68DD129 pKa = 3.27KK130 pKa = 10.28PFKK133 pKa = 10.85GFASGLHH140 pKa = 5.0VVNLLMGAILLYY152 pKa = 9.99CAKK155 pKa = 10.2GVGKK159 pKa = 9.95IRR161 pKa = 11.84EE162 pKa = 4.1EE163 pKa = 3.76MRR165 pKa = 11.84QLEE168 pKa = 4.14RR169 pKa = 11.84DD170 pKa = 3.55LEE172 pKa = 4.23KK173 pKa = 10.85KK174 pKa = 10.23IMYY177 pKa = 10.03NSVAGRR183 pKa = 11.84TLGIQRR189 pKa = 11.84QHH191 pKa = 3.83VTMYY195 pKa = 10.37EE196 pKa = 4.03PANEE200 pKa = 4.31TMKK203 pKa = 10.63TMPLYY208 pKa = 6.74PTHH211 pKa = 6.27YY212 pKa = 10.27VV213 pKa = 3.09

Molecular weight: 23.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5962

213

1321

596.2

68.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.24 ± 0.511

1.342 ± 0.28

5.585 ± 0.407

7.296 ± 0.566

3.807 ± 0.217

5.938 ± 0.288

2.499 ± 0.211

6.189 ± 0.334

5.669 ± 0.638

9.074 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.69 ± 0.328

4.143 ± 0.319

3.556 ± 0.419

3.975 ± 0.286

7.112 ± 0.406

6.156 ± 0.333

5.552 ± 0.242

7.045 ± 0.46

1.124 ± 0.276

3.992 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski