Apostasia shenzhenica

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; Asparagales; Orchidaceae; Apostasioideae; Apostasia

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0BB82|A0A2I0BB82_9ASPA Pentatricopeptide repeat-containing protein OS=Apostasia shenzhenica OX=1088818 GN=PCMP-H51 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 4.95CSGGEE7 pKa = 4.12SAVPLASADD16 pKa = 3.59PPRR19 pKa = 11.84DD20 pKa = 3.82YY21 pKa = 10.76IEE23 pKa = 4.86HH24 pKa = 6.2VGDD27 pKa = 4.19SEE29 pKa = 5.04EE30 pKa = 4.36ISCSGAEE37 pKa = 3.93EE38 pKa = 4.26TGDD41 pKa = 3.82EE42 pKa = 4.29GCEE45 pKa = 3.76ADD47 pKa = 5.01DD48 pKa = 5.66AEE50 pKa = 4.46SCIIGGAGDD59 pKa = 4.02RR60 pKa = 11.84DD61 pKa = 3.62QGCISWRR68 pKa = 11.84MWCILGHH75 pKa = 5.04EE76 pKa = 4.41TWGGGRR82 pKa = 11.84WRR84 pKa = 11.84GSCGGDD90 pKa = 2.9GRR92 pKa = 11.84SNSDD96 pKa = 2.95SSALTVDD103 pKa = 5.42DD104 pKa = 4.01EE105 pKa = 4.85AEE107 pKa = 4.05EE108 pKa = 4.67NRR110 pKa = 11.84LFWEE114 pKa = 4.41ACLADD119 pKa = 4.75EE120 pKa = 5.29SPLPPSFF127 pKa = 4.32

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H9ZYP5|A0A2H9ZYP5_9ASPA Uncharacterized protein OS=Apostasia shenzhenica OX=1088818 GN=AXF42_Ash020936 PE=4 SV=1
MM1 pKa = 7.48QFMGFMRR8 pKa = 11.84FTGFARR14 pKa = 11.84FAGFAQFARR23 pKa = 11.84FVRR26 pKa = 11.84FVGFVWFVGFTRR38 pKa = 11.84FAVFAHH44 pKa = 5.66FVVFARR50 pKa = 11.84FAGFPGFALFTRR62 pKa = 11.84FAGFARR68 pKa = 11.84FLL70 pKa = 3.92

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21679

0

21679

7935319

49

5082

366.0

40.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.773 ± 0.019

1.938 ± 0.008

5.073 ± 0.013

6.368 ± 0.019

4.325 ± 0.013

6.767 ± 0.019

2.375 ± 0.007

5.208 ± 0.011

5.505 ± 0.016

9.939 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.007

3.836 ± 0.01

5.125 ± 0.019

3.407 ± 0.012

6.093 ± 0.016

9.013 ± 0.021

4.482 ± 0.01

6.412 ± 0.012

1.294 ± 0.006

2.655 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski