Gordonia phage Sidious

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nymbaxtervirinae; unclassified Nymbaxtervirinae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A515MIB4|A0A515MIB4_9CAUD DNA polymerase III subunit OS=Gordonia phage Sidious OX=2591118 GN=59 PE=4 SV=1
MM1 pKa = 7.65TIHH4 pKa = 5.88TTADD8 pKa = 3.8LDD10 pKa = 4.73LGDD13 pKa = 4.5AVAGVLTSIAANRR26 pKa = 11.84PATISPLDD34 pKa = 3.68IDD36 pKa = 5.1DD37 pKa = 4.76GPGARR42 pKa = 11.84IRR44 pKa = 11.84FAVDD48 pKa = 3.44LGAVADD54 pKa = 3.95TAEE57 pKa = 4.39VIVPFTALAEE67 pKa = 4.26YY68 pKa = 10.13VDD70 pKa = 3.89QVRR73 pKa = 11.84ASTEE77 pKa = 3.74PSS79 pKa = 3.01

Molecular weight: 8.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A515MIB1|A0A515MIB1_9CAUD Uncharacterized protein OS=Gordonia phage Sidious OX=2591118 GN=51 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84KK3 pKa = 8.89ILKK6 pKa = 9.8ILEE9 pKa = 4.03ARR11 pKa = 11.84PYY13 pKa = 9.1VVTVAIVVLGIAEE26 pKa = 4.41FVTAQRR32 pKa = 11.84DD33 pKa = 3.57WFTSAVGNLLRR44 pKa = 11.84DD45 pKa = 3.7ASDD48 pKa = 3.69GAGVAVALGMSSVAAIVAGFVGVIVVFGLGSDD80 pKa = 3.42NAIIISFRR88 pKa = 11.84HH89 pKa = 4.41RR90 pKa = 11.84TGSVMRR96 pKa = 11.84SNWISVIGSSFASAILGFVGAILLAASSYY125 pKa = 10.01QLAGAVLCVGMLLLCHH141 pKa = 6.96AIIRR145 pKa = 11.84TLWLFGVLMTVIATGDD161 pKa = 3.2ARR163 pKa = 11.84EE164 pKa = 4.02DD165 pKa = 3.04RR166 pKa = 11.84RR167 pKa = 11.84RR168 pKa = 11.84RR169 pKa = 11.84TRR171 pKa = 11.84STSDD175 pKa = 2.44IFRR178 pKa = 11.84PAAA181 pKa = 3.7

Molecular weight: 19.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16881

39

2157

213.7

23.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.322 ± 0.567

0.717 ± 0.148

6.966 ± 0.332

5.764 ± 0.288

2.713 ± 0.165

8.364 ± 0.314

1.931 ± 0.167

4.496 ± 0.204

3.187 ± 0.204

7.778 ± 0.22

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.127 ± 0.143

3.045 ± 0.22

5.45 ± 0.248

3.513 ± 0.286

7.511 ± 0.421

5.669 ± 0.204

7.15 ± 0.274

7.381 ± 0.287

1.842 ± 0.149

2.073 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski