Vibrio phage vB_ValS_X1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Pogseptimavirus; unclassified Pogseptimavirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9Z6K2|A0A6M9Z6K2_9CAUD Uncharacterized protein OS=Vibrio phage vB_ValS_X1 OX=2736341 GN=vBValSX1_35 PE=4 SV=1
MM1 pKa = 7.55FNQKK5 pKa = 10.3DD6 pKa = 3.5KK7 pKa = 11.76ANLVTLDD14 pKa = 3.99YY15 pKa = 10.95ILAEE19 pKa = 4.19LSMLSATDD27 pKa = 4.2DD28 pKa = 4.03PSDD31 pKa = 3.8TVWYY35 pKa = 9.56IDD37 pKa = 3.51SDD39 pKa = 3.83EE40 pKa = 4.47SLLLTVHH47 pKa = 6.83PKK49 pKa = 10.31YY50 pKa = 11.11GLVFYY55 pKa = 10.58NDD57 pKa = 3.94DD58 pKa = 3.35MHH60 pKa = 6.98TLGIYY65 pKa = 10.4DD66 pKa = 4.37KK67 pKa = 9.52EE68 pKa = 4.28QEE70 pKa = 4.38SEE72 pKa = 4.34ILDD75 pKa = 4.78LLDD78 pKa = 3.93DD79 pKa = 4.08WSIYY83 pKa = 10.42SDD85 pKa = 3.34SKK87 pKa = 9.17VWRR90 pKa = 11.84EE91 pKa = 3.66IGIDD95 pKa = 3.33QVDD98 pKa = 3.81YY99 pKa = 11.59VIVV102 pKa = 3.46

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z606|A0A6M9Z606_9CAUD Helicase C terminal domain OS=Vibrio phage vB_ValS_X1 OX=2736341 GN=vBValSX1_14 PE=4 SV=1
MM1 pKa = 7.8ISRR4 pKa = 11.84KK5 pKa = 8.0IRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84IDD11 pKa = 3.06EE12 pKa = 3.92NRR14 pKa = 11.84NLEE17 pKa = 3.99KK18 pKa = 10.7SRR20 pKa = 11.84QVEE23 pKa = 3.99AAKK26 pKa = 10.06RR27 pKa = 11.84AYY29 pKa = 10.32RR30 pKa = 11.84EE31 pKa = 3.8IVLALGDD38 pKa = 3.7EE39 pKa = 4.44RR40 pKa = 11.84EE41 pKa = 4.1NFLVGRR47 pKa = 11.84YY48 pKa = 6.86TMYY51 pKa = 10.56RR52 pKa = 11.84EE53 pKa = 3.68SDD55 pKa = 2.92IKK57 pKa = 10.54YY58 pKa = 8.37VRR60 pKa = 11.84QILRR64 pKa = 11.84YY65 pKa = 8.85LGVSAKK71 pKa = 10.46VYY73 pKa = 9.15VVPIQGRR80 pKa = 11.84LMDD83 pKa = 4.48CRR85 pKa = 11.84NVITSTGFSIAVQHH99 pKa = 6.12GG100 pKa = 3.32

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

170

0

170

33141

39

1598

194.9

21.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.857 ± 0.282

1.195 ± 0.107

6.412 ± 0.142

7.74 ± 0.265

3.769 ± 0.105

6.285 ± 0.224

1.964 ± 0.126

5.658 ± 0.143

6.493 ± 0.258

9.064 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.498 ± 0.125

4.599 ± 0.148

3.633 ± 0.147

3.437 ± 0.121

4.789 ± 0.146

6.309 ± 0.251

6.192 ± 0.244

6.88 ± 0.141

1.273 ± 0.075

3.953 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski