Nippostrongylus brasiliensis (Rat hookworm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Strongylida; Trichostrongyloidea; Heligmonellidae; Nippostrongylinae; Nippostrongylus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22636 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N4YQK6|A0A0N4YQK6_NIPBR Tubulin alpha chain OS=Nippostrongylus brasiliensis OX=27835 GN=NBR_LOCUS19529 PE=3 SV=1
MM1 pKa = 7.31IACSALGPMDD11 pKa = 4.66SLPAAKK17 pKa = 9.48TNYY20 pKa = 9.42VDD22 pKa = 3.12THH24 pKa = 6.47SRR26 pKa = 11.84EE27 pKa = 4.39SYY29 pKa = 10.11AAPPPAPAPPPPASGYY45 pKa = 10.5AVAPAPPVIAPPPPPPPPPPPAPAPYY71 pKa = 9.56VPQVPPPSFAADD83 pKa = 3.06HH84 pKa = 6.36GSYY87 pKa = 10.52AGAGLGVGGLGGGGGSYY104 pKa = 9.92QRR106 pKa = 11.84PAPVITPVAPQPTYY120 pKa = 10.58QGSQAIAPTPQTFQSTVQTNAGVDD144 pKa = 3.53LGTSAGQIQGPTYY157 pKa = 9.9QQVPAEE163 pKa = 4.32NIQPVVEE170 pKa = 4.35QTATAEE176 pKa = 4.33VQPSAPEE183 pKa = 4.09TVEE186 pKa = 4.04TPIDD190 pKa = 3.73VQGQQPTVIEE200 pKa = 4.19QTEE203 pKa = 4.36SLPSEE208 pKa = 4.27TAQVATATEE217 pKa = 4.09AAKK220 pKa = 9.13GTEE223 pKa = 3.94EE224 pKa = 4.46SSYY227 pKa = 11.68DD228 pKa = 4.28DD229 pKa = 3.77IVEE232 pKa = 4.08EE233 pKa = 4.02QQAVDD238 pKa = 3.85SVNTIPNSSPSTNDD252 pKa = 2.11VDD254 pKa = 4.14YY255 pKa = 9.65PADD258 pKa = 4.23GGDD261 pKa = 4.13EE262 pKa = 4.36GNCDD266 pKa = 4.16DD267 pKa = 5.9LEE269 pKa = 4.45LKK271 pKa = 10.77AIVEE275 pKa = 4.36GALGAEE281 pKa = 4.75KK282 pKa = 10.31MMEE285 pKa = 4.41DD286 pKa = 4.54PSRR289 pKa = 11.84MMEE292 pKa = 4.73DD293 pKa = 3.73PMTMMDD299 pKa = 4.52HH300 pKa = 6.89FRR302 pKa = 11.84TGDD305 pKa = 3.2KK306 pKa = 10.94DD307 pKa = 3.28RR308 pKa = 5.97

Molecular weight: 31.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N4YDZ8|A0A0N4YDZ8_NIPBR FOG-3 protein (inferred by orthology to a C. elegans protein) OS=Nippostrongylus brasiliensis OX=27835 GN=NBR_LOCUS14883 PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 10.31KK3 pKa = 10.39IMVTMMMKK11 pKa = 10.09RR12 pKa = 11.84RR13 pKa = 11.84WRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 7.07RR31 pKa = 11.84TKK33 pKa = 8.76RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.43RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 6.64RR40 pKa = 11.84TKK42 pKa = 8.72RR43 pKa = 11.84RR44 pKa = 11.84KK45 pKa = 7.72RR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 7.64RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84EE60 pKa = 3.57RR61 pKa = 11.84KK62 pKa = 8.96KK63 pKa = 11.02KK64 pKa = 9.91KK65 pKa = 9.71ICWLQGLVKK74 pKa = 10.52IKK76 pKa = 10.81

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22636

0

22636

6447329

29

6471

284.8

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.173 ± 0.016

2.145 ± 0.015

5.528 ± 0.013

6.664 ± 0.024

4.182 ± 0.014

5.729 ± 0.018

2.382 ± 0.008

5.209 ± 0.015

5.695 ± 0.018

9.027 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.009

4.061 ± 0.011

5.052 ± 0.019

3.893 ± 0.014

6.234 ± 0.016

7.778 ± 0.021

5.555 ± 0.016

6.888 ± 0.015

1.165 ± 0.006

3.006 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski