Synechococcus phage S-CBS4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2BCU7|H2BCU7_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBS4 OX=756275 GN=S-CBS4_gp056 PE=4 SV=1
MM1 pKa = 7.35AQFTIDD7 pKa = 4.45IPDD10 pKa = 3.75EE11 pKa = 4.12LLPALVAEE19 pKa = 5.09FSLVQGSTTATTPEE33 pKa = 4.25EE34 pKa = 4.1YY35 pKa = 10.11FAASVVEE42 pKa = 4.39TVRR45 pKa = 11.84QRR47 pKa = 11.84AEE49 pKa = 3.97LYY51 pKa = 10.58KK52 pKa = 10.68VGPYY56 pKa = 9.8YY57 pKa = 10.76AGPVDD62 pKa = 4.16PQFQADD68 pKa = 4.03GKK70 pKa = 8.88PYY72 pKa = 9.44GWVAPVVDD80 pKa = 4.73NDD82 pKa = 3.61TTEE85 pKa = 4.26PDD87 pKa = 3.45GGDD90 pKa = 3.24VV91 pKa = 3.56

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2BCR1|H2BCR1_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBS4 OX=756275 GN=S-CBS4_gp020 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84SAARR6 pKa = 11.84RR7 pKa = 11.84GLEE10 pKa = 3.94LRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 8.57HH15 pKa = 6.18GKK17 pKa = 10.26GGLTTKK23 pKa = 10.33EE24 pKa = 3.94AGKK27 pKa = 10.24QGIGSGVARR36 pKa = 11.84ATSLANGDD44 pKa = 3.73KK45 pKa = 10.93VSAEE49 pKa = 4.16TIRR52 pKa = 11.84RR53 pKa = 11.84MVAFFSRR60 pKa = 11.84HH61 pKa = 5.05EE62 pKa = 4.34KK63 pKa = 10.3NKK65 pKa = 10.93SGGEE69 pKa = 3.86DD70 pKa = 3.37DD71 pKa = 4.34AGYY74 pKa = 10.08IAWLLWGGDD83 pKa = 3.73AGKK86 pKa = 10.14SWANRR91 pKa = 11.84TLRR94 pKa = 11.84MLEE97 pKa = 3.75KK98 pKa = 10.17RR99 pKa = 11.84QQKK102 pKa = 9.78

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

21153

31

1451

201.5

22.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.928 ± 0.415

1.078 ± 0.143

6.094 ± 0.221

6.146 ± 0.373

3.721 ± 0.148

8.76 ± 0.492

1.584 ± 0.182

5.46 ± 0.175

4.765 ± 0.294

7.971 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.146

4.557 ± 0.302

4.198 ± 0.176

3.801 ± 0.243

5.37 ± 0.254

7.2 ± 0.305

6.212 ± 0.537

6.349 ± 0.201

1.38 ± 0.137

3.238 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski