Circovirus-like genome RW-A

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GIH6|C6GIH6_9VIRU Uncharacterized protein OS=Circovirus-like genome RW-A OX=642251 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 4.71WEE4 pKa = 4.46STQSEE9 pKa = 4.7DD10 pKa = 4.1LTDD13 pKa = 3.46VSLDD17 pKa = 3.45SSEE20 pKa = 4.79EE21 pKa = 4.01GLSNSDD27 pKa = 3.49AYY29 pKa = 10.37EE30 pKa = 4.36GSSDD34 pKa = 3.7EE35 pKa = 4.79AEE37 pKa = 4.41STQSEE42 pKa = 4.04QDD44 pKa = 3.25FPQRR48 pKa = 11.84IPSEE52 pKa = 4.17TRR54 pKa = 11.84LMSSSGSIEE63 pKa = 4.08LATSQEE69 pKa = 3.95MEE71 pKa = 4.28RR72 pKa = 11.84PLKK75 pKa = 10.07RR76 pKa = 11.84QRR78 pKa = 11.84LTEE81 pKa = 4.18PSTTSLTHH89 pKa = 7.0GYY91 pKa = 9.16PLSQSQKK98 pKa = 9.72DD99 pKa = 3.57FSPILSSSEE108 pKa = 4.2GIKK111 pKa = 9.26STEE114 pKa = 3.77SWSNSQSTNSRR125 pKa = 11.84TPPEE129 pKa = 4.46AGSFQALHH137 pKa = 6.59GSCHH141 pKa = 5.29IRR143 pKa = 11.84RR144 pKa = 11.84STEE147 pKa = 3.61HH148 pKa = 6.7LPSSRR153 pKa = 11.84KK154 pKa = 7.14TLSRR158 pKa = 11.84LSLNAIRR165 pKa = 11.84RR166 pKa = 11.84GLMSQIGGMGGGQRR180 pKa = 11.84QQ181 pKa = 3.08

Molecular weight: 19.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GIH6|C6GIH6_9VIRU Uncharacterized protein OS=Circovirus-like genome RW-A OX=642251 PE=4 SV=1
MM1 pKa = 7.48GKK3 pKa = 7.62YY4 pKa = 8.17TKK6 pKa = 10.02RR7 pKa = 11.84RR8 pKa = 11.84SYY10 pKa = 10.69GRR12 pKa = 11.84KK13 pKa = 8.82SRR15 pKa = 11.84LKK17 pKa = 9.49RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84SVKK23 pKa = 7.96QRR25 pKa = 11.84RR26 pKa = 11.84IRR28 pKa = 11.84RR29 pKa = 11.84IKK31 pKa = 9.92RR32 pKa = 11.84RR33 pKa = 11.84SGIYY37 pKa = 8.64TKK39 pKa = 9.98RR40 pKa = 11.84TRR42 pKa = 11.84FPAKK46 pKa = 10.51NPFGDD51 pKa = 3.3KK52 pKa = 10.6AYY54 pKa = 11.16VKK56 pKa = 10.62LRR58 pKa = 11.84FNRR61 pKa = 11.84ASYY64 pKa = 10.68ISGDD68 pKa = 3.68GASSQTTTTYY78 pKa = 9.66RR79 pKa = 11.84TINNLADD86 pKa = 3.11TWLSFEE92 pKa = 4.34SVSKK96 pKa = 11.11GFLTYY101 pKa = 10.01PKK103 pKa = 10.22LFRR106 pKa = 11.84RR107 pKa = 11.84YY108 pKa = 8.76KK109 pKa = 9.94VNGVMVKK116 pKa = 8.29FTVYY120 pKa = 9.88QLKK123 pKa = 10.55NSTGSGEE130 pKa = 4.3FPSLAWILPYY140 pKa = 9.72STLDD144 pKa = 3.23GTPTVFAQNAIALKK158 pKa = 8.68SQRR161 pKa = 11.84HH162 pKa = 4.29TAWANVANWGNGGRR176 pKa = 11.84STTVKK181 pKa = 10.44KK182 pKa = 10.02FFKK185 pKa = 10.05MKK187 pKa = 10.43SLVGANYY194 pKa = 9.13PSTDD198 pKa = 2.66IDD200 pKa = 3.99YY201 pKa = 11.32SGTTDD206 pKa = 4.0VLANPYY212 pKa = 9.1NAPAIEE218 pKa = 3.74WRR220 pKa = 11.84FLAGISSVAEE230 pKa = 3.83VALPTTQSYY239 pKa = 10.53HH240 pKa = 6.46YY241 pKa = 8.97NLEE244 pKa = 3.53MTYY247 pKa = 10.54YY248 pKa = 10.57VEE250 pKa = 4.95FWEE253 pKa = 4.53QTWEE257 pKa = 4.08AQGLL261 pKa = 3.85

Molecular weight: 29.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

788

181

346

262.7

29.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.964 ± 0.992

0.508 ± 0.266

5.33 ± 1.729

5.33 ± 1.581

4.822 ± 1.123

7.741 ± 0.575

1.777 ± 0.493

3.553 ± 0.226

4.822 ± 1.393

7.868 ± 0.652

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.65 ± 0.383

3.299 ± 0.997

5.203 ± 0.778

4.822 ± 0.954

8.249 ± 0.372

10.152 ± 3.904

7.487 ± 1.167

4.188 ± 1.216

2.919 ± 0.73

4.315 ± 1.24
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski