Phyllobacterium zundukense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Phyllobacterium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5605 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N9VW59|A0A2N9VW59_9RHIZ Uncharacterized protein OS=Phyllobacterium zundukense OX=1867719 GN=B5P45_17710 PE=4 SV=1
MM1 pKa = 7.39APAVAFADD9 pKa = 4.18CVTLGGVITCDD20 pKa = 3.03TAAPNPFTSTVGAGNTAAADD40 pKa = 3.98NITVNVGPGAQVAVGNLNAISLRR63 pKa = 11.84NNAVITIAGGGLVSATATSTTGNYY87 pKa = 7.37NTGGNAVEE95 pKa = 4.39VNNNGTLTIEE105 pKa = 4.4QGGQLLALGTQGSAEE120 pKa = 3.82AVNFQGTGNVLTNNGTIDD138 pKa = 3.65ANNSVAIWSQNTSGLNTVINNEE160 pKa = 4.0TGIIEE165 pKa = 4.59AGNGTTSTVIGGSGAGALDD184 pKa = 3.52FTNRR188 pKa = 11.84GTIRR192 pKa = 11.84GSINLANGNDD202 pKa = 3.4ILRR205 pKa = 11.84LYY207 pKa = 9.46TGSTVTGNFSGGGGTDD223 pKa = 4.04AIFLSGDD230 pKa = 3.77GQSSMAGNFNGFEE243 pKa = 4.18SLVKK247 pKa = 10.44NDD249 pKa = 3.7LGTWTLTGTITGVTVATVQNGTLVLTGANTNYY281 pKa = 9.01TGQVIIDD288 pKa = 3.6PAGTLEE294 pKa = 4.5ARR296 pKa = 11.84AQALPTQLLPANNVANITNNGLVRR320 pKa = 11.84FAQPDD325 pKa = 3.12NGTYY329 pKa = 10.03VGQIVGTGAVEE340 pKa = 3.87KK341 pKa = 9.83TGAGTLTLEE350 pKa = 4.39PSVAAGNTYY359 pKa = 10.78SGGTFINGGVVAVGADD375 pKa = 3.45NALGASTGGVSFNGGTLQLNSSFNLSAGRR404 pKa = 11.84AVTLNVGGGTINTQAFDD421 pKa = 3.38STLAQGVTGPGGLTKK436 pKa = 10.68AGAGTLTLTGNSDD449 pKa = 3.54YY450 pKa = 11.64SGGTVISAGTLQLGDD465 pKa = 4.02SGTSGSISGDD475 pKa = 3.33VLNNSLLAFNRR486 pKa = 11.84SDD488 pKa = 3.23AFLVPGVISGSGAVEE503 pKa = 3.92QNGSGTTILTGNNTYY518 pKa = 10.94SGGTTINAGTLQLGNGGTSGSIVGDD543 pKa = 3.6VANDD547 pKa = 3.39GSLAFNRR554 pKa = 11.84SDD556 pKa = 3.16STNFDD561 pKa = 3.59GVISSAGNVRR571 pKa = 11.84QIGGGTTILTAQNTYY586 pKa = 10.87SGATTVEE593 pKa = 4.03AGTLAAGATNVFSPNSSTSVLTGGTLDD620 pKa = 4.32AAGFDD625 pKa = 3.77QTVSALTNAGLVNVGGAPGTTLTVSGNYY653 pKa = 9.6VGADD657 pKa = 3.05GTLRR661 pKa = 11.84LNTALGDD668 pKa = 4.04DD669 pKa = 3.94ASTTDD674 pKa = 3.42RR675 pKa = 11.84LVVLGDD681 pKa = 3.67TSGSTTLVVANVGGSGAPTVEE702 pKa = 4.91GIRR705 pKa = 11.84VIDD708 pKa = 3.73VAGSSAGSFALDD720 pKa = 2.68GDD722 pKa = 4.33YY723 pKa = 11.36AFEE726 pKa = 4.8GDD728 pKa = 3.52EE729 pKa = 4.03AVIGGAYY736 pKa = 10.14AYY738 pKa = 10.24RR739 pKa = 11.84LYY741 pKa = 11.03KK742 pKa = 10.63NGIATPTDD750 pKa = 3.69GNWYY754 pKa = 9.8LRR756 pKa = 11.84SALVDD761 pKa = 3.64PADD764 pKa = 3.86PTAPTDD770 pKa = 3.67PTDD773 pKa = 3.87PTDD776 pKa = 3.89PTDD779 pKa = 3.76PTSPSSPLYY788 pKa = 9.64QPGVPVYY795 pKa = 9.58EE796 pKa = 5.31AYY798 pKa = 10.36AQTLLGLNGLPTLQQRR814 pKa = 11.84VGNRR818 pKa = 11.84YY819 pKa = 8.54WSGAGNGMLSQGDD832 pKa = 4.11GPGTIEE838 pKa = 4.97PAPQPSDD845 pKa = 3.12GGPAFVEE852 pKa = 5.1GRR854 pKa = 11.84GLWARR859 pKa = 11.84IEE861 pKa = 4.2GAHH864 pKa = 6.62GNFDD868 pKa = 3.87PRR870 pKa = 11.84TSTSSTDD877 pKa = 3.23YY878 pKa = 11.57DD879 pKa = 4.26LDD881 pKa = 3.3TWKK884 pKa = 10.27IQSGLDD890 pKa = 3.37GQLYY894 pKa = 9.89EE895 pKa = 4.52SEE897 pKa = 4.19SGKK900 pKa = 10.92LIGGITAQYY909 pKa = 10.11GNASADD915 pKa = 3.5VSSLFGNGEE924 pKa = 4.02VDD926 pKa = 2.95TDD928 pKa = 4.33GYY930 pKa = 11.6GVGATLTWYY939 pKa = 9.28GQNGFYY945 pKa = 10.78LDD947 pKa = 4.01GQAQATWYY955 pKa = 10.24EE956 pKa = 4.13SDD958 pKa = 3.81LSSGVLGSLSNGNDD972 pKa = 3.04GFGYY976 pKa = 10.62AFSLEE981 pKa = 4.2TGKK984 pKa = 10.72RR985 pKa = 11.84LALNQNWTLTPQAQLAYY1002 pKa = 10.92SNVDD1006 pKa = 3.88FDD1008 pKa = 5.56AFTDD1012 pKa = 3.85PFGADD1017 pKa = 3.01VSLGTGEE1024 pKa = 4.21SLKK1027 pKa = 10.89GRR1029 pKa = 11.84LGLSADD1035 pKa = 4.36YY1036 pKa = 11.05EE1037 pKa = 4.51NAWEE1041 pKa = 4.45GASGRR1046 pKa = 11.84TTRR1049 pKa = 11.84THH1051 pKa = 7.25LYY1053 pKa = 10.65GIANLYY1059 pKa = 10.82YY1060 pKa = 10.05EE1061 pKa = 4.84FLDD1064 pKa = 3.93GTEE1067 pKa = 4.22VEE1069 pKa = 4.39VSGVNFASEE1078 pKa = 4.2NDD1080 pKa = 3.97RR1081 pKa = 11.84TWGGIGTGGSYY1092 pKa = 10.33NWNDD1096 pKa = 3.7DD1097 pKa = 3.21KK1098 pKa = 11.77YY1099 pKa = 10.78SIYY1102 pKa = 10.97SEE1104 pKa = 4.38VSVNTSLSNFADD1116 pKa = 3.78SYY1118 pKa = 10.87SLNGTAGFRR1127 pKa = 11.84VKK1129 pKa = 10.46FF1130 pKa = 3.8

Molecular weight: 113.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N9VSU7|A0A2N9VSU7_9RHIZ YkuD domain-containing protein OS=Phyllobacterium zundukense OX=1867719 GN=B5P45_23120 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.15VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5605

0

5605

1679408

23

3389

299.6

32.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.314 ± 0.041

0.802 ± 0.009

5.673 ± 0.029

5.724 ± 0.033

3.976 ± 0.022

8.203 ± 0.029

2.077 ± 0.015

5.99 ± 0.021

4.002 ± 0.029

9.87 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.016

3.072 ± 0.02

4.842 ± 0.025

3.167 ± 0.017

6.402 ± 0.028

5.84 ± 0.023

5.491 ± 0.027

7.341 ± 0.028

1.297 ± 0.012

2.403 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski