Vibrio phage R01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mardecavirus; unclassified Mardecavirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MCU0|A0A345MCU0_9CAUD Uncharacterized protein OS=Vibrio phage R01 OX=2283098 PE=4 SV=1
MM1 pKa = 7.99AIQLQIRR8 pKa = 11.84VSAEE12 pKa = 3.58LEE14 pKa = 4.04AEE16 pKa = 4.13QFPLCPYY23 pKa = 10.33CDD25 pKa = 3.54QPLQNANDD33 pKa = 3.75LVIVYY38 pKa = 10.54NGMFSVMALAHH49 pKa = 5.48QSCHH53 pKa = 6.98DD54 pKa = 4.14EE55 pKa = 4.15NDD57 pKa = 3.36SS58 pKa = 3.73

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MD17|A0A345MD17_9CAUD Uncharacterized protein OS=Vibrio phage R01 OX=2283098 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84RR3 pKa = 11.84TRR5 pKa = 11.84KK6 pKa = 9.73VMPKK10 pKa = 9.51PIMWSMEE17 pKa = 4.21EE18 pKa = 4.35IIEE21 pKa = 4.35SPNRR25 pKa = 11.84VVPALLTSSYY35 pKa = 10.91LYY37 pKa = 10.43YY38 pKa = 10.81LRR40 pKa = 11.84GDD42 pKa = 3.7MSPIMRR48 pKa = 11.84DD49 pKa = 2.95EE50 pKa = 6.25DD51 pKa = 4.08FDD53 pKa = 3.79QCCRR57 pKa = 11.84LLRR60 pKa = 11.84RR61 pKa = 11.84KK62 pKa = 9.65YY63 pKa = 10.42RR64 pKa = 11.84EE65 pKa = 3.36ITHH68 pKa = 5.7PHH70 pKa = 6.04KK71 pKa = 11.05SLIKK75 pKa = 10.2MSDD78 pKa = 3.28LSAGTLFRR86 pKa = 11.84LRR88 pKa = 11.84DD89 pKa = 3.51HH90 pKa = 7.84DD91 pKa = 4.19YY92 pKa = 8.1PTIVQVVAVEE102 pKa = 4.07MSMGTLYY109 pKa = 10.52DD110 pKa = 3.74KK111 pKa = 11.02PMPTPKK117 pKa = 8.94PTAEE121 pKa = 3.57KK122 pKa = 9.6RR123 pKa = 11.84VRR125 pKa = 11.84KK126 pKa = 9.57RR127 pKa = 11.84RR128 pKa = 11.84VRR130 pKa = 11.84NNGGSSSGPKK140 pKa = 10.09GFLNRR145 pKa = 11.84RR146 pKa = 11.84RR147 pKa = 11.84RR148 pKa = 3.47

Molecular weight: 17.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

22733

46

948

225.1

25.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.843 ± 0.434

1.258 ± 0.115

6.603 ± 0.273

7.153 ± 0.337

4.065 ± 0.137

7.003 ± 0.182

2.116 ± 0.136

5.305 ± 0.145

6.506 ± 0.233

8.472 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.143

4.285 ± 0.164

3.95 ± 0.203

3.603 ± 0.158

5.573 ± 0.238

5.723 ± 0.21

6.013 ± 0.235

6.823 ± 0.221

1.526 ± 0.096

3.515 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski