Pseudomonas phage Henu5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pakpunavirus; unclassified Pakpunavirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410T8D1|A0A410T8D1_9CAUD Uncharacterized protein OS=Pseudomonas phage Henu5 OX=2499902 GN=Henu5_gp144 PE=4 SV=1
MM1 pKa = 7.62SILALTLTLCSAVQCDD17 pKa = 5.16DD18 pKa = 4.29YY19 pKa = 11.33IIDD22 pKa = 4.03HH23 pKa = 6.42SLPGQAAEE31 pKa = 4.28CNARR35 pKa = 11.84LVEE38 pKa = 4.04EE39 pKa = 4.46AEE41 pKa = 4.25EE42 pKa = 5.5FGDD45 pKa = 4.02AWVATNADD53 pKa = 3.46ARR55 pKa = 11.84LTRR58 pKa = 11.84YY59 pKa = 9.98LSRR62 pKa = 11.84FNIQVDD68 pKa = 3.36PRR70 pKa = 11.84FVFDD74 pKa = 5.54YY75 pKa = 11.21DD76 pKa = 3.86FTCQLIAEE84 pKa = 4.86DD85 pKa = 4.44EE86 pKa = 4.45LPP88 pKa = 4.02

Molecular weight: 9.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410T888|A0A410T888_9CAUD Uncharacterized protein OS=Pseudomonas phage Henu5 OX=2499902 GN=Henu5_gp155 PE=4 SV=1
MM1 pKa = 7.31LWNRR5 pKa = 11.84TTYY8 pKa = 10.6RR9 pKa = 11.84SAARR13 pKa = 11.84ARR15 pKa = 11.84YY16 pKa = 9.75SRR18 pKa = 11.84FTGLRR23 pKa = 11.84SRR25 pKa = 11.84EE26 pKa = 4.13PKK28 pKa = 10.0SVNAIYY34 pKa = 10.38HH35 pKa = 5.43CTTLVGGGDD44 pKa = 3.89SNPNNRR50 pKa = 11.84VTTEE54 pKa = 4.03RR55 pKa = 11.84QHH57 pKa = 6.21PGYY60 pKa = 9.02TISTRR65 pKa = 11.84IMPSRR70 pKa = 11.84LKK72 pKa = 10.95AHH74 pKa = 6.02TEE76 pKa = 3.91EE77 pKa = 5.17CSGLDD82 pKa = 4.01LIGAVTGDD90 pKa = 3.23RR91 pKa = 11.84TRR93 pKa = 11.84NTRR96 pKa = 11.84LKK98 pKa = 8.54VWRR101 pKa = 11.84FIHH104 pKa = 7.23LIYY107 pKa = 10.97NCIVWW112 pKa = 3.44

Molecular weight: 12.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

145

0

145

26384

67

788

182.0

20.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.028 ± 0.289

1.395 ± 0.106

5.753 ± 0.174

7.084 ± 0.274

4.321 ± 0.123

7.0 ± 0.21

2.085 ± 0.134

5.545 ± 0.177

5.666 ± 0.246

8.426 ± 0.186

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.968 ± 0.123

4.059 ± 0.173

3.858 ± 0.134

3.502 ± 0.134

5.795 ± 0.16

6.159 ± 0.169

5.685 ± 0.214

7.451 ± 0.246

1.668 ± 0.098

3.532 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski