Sulfolobus spindle-shaped virus 6

Taxonomy: Viruses; Fuselloviridae; Betafusellovirus

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1GF38|D1GF38_9VIRU Uncharacterized protein OS=Sulfolobus spindle-shaped virus 6 OX=693627 PE=4 SV=1
MM1 pKa = 8.17SEE3 pKa = 3.7EE4 pKa = 4.56LEE6 pKa = 4.32YY7 pKa = 10.5IKK9 pKa = 10.85QKK11 pKa = 10.67LDD13 pKa = 4.09EE14 pKa = 5.0ILDD17 pKa = 3.78EE18 pKa = 5.38CICSDD23 pKa = 3.82PEE25 pKa = 3.8EE26 pKa = 4.11LRR28 pKa = 11.84EE29 pKa = 4.77LEE31 pKa = 4.55EE32 pKa = 3.79ILKK35 pKa = 10.46KK36 pKa = 10.08IEE38 pKa = 4.04YY39 pKa = 9.83VLGYY43 pKa = 10.1CQMGDD48 pKa = 2.99

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1GF47|D1GF47_9VIRU Putative end-filament protein OS=Sulfolobus spindle-shaped virus 6 OX=693627 PE=4 SV=1
MM1 pKa = 7.52GVRR4 pKa = 11.84FSYY7 pKa = 10.7RR8 pKa = 11.84LNFHH12 pKa = 6.19GNRR15 pKa = 11.84VEE17 pKa = 4.31IYY19 pKa = 7.14GTCRR23 pKa = 11.84LSSIRR28 pKa = 11.84RR29 pKa = 11.84CFSLLSKK36 pKa = 9.48FLWSYY41 pKa = 9.72TLLHH45 pKa = 6.27HH46 pKa = 6.27LHH48 pKa = 6.52FRR50 pKa = 11.84FHH52 pKa = 7.36SCGNWSKK59 pKa = 11.69SNNKK63 pKa = 9.28AIWWRR68 pKa = 3.6

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33

0

33

5033

48

1232

152.5

17.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.007 ± 0.312

0.576 ± 0.249

3.477 ± 0.347

6.06 ± 0.872

5.007 ± 0.385

5.762 ± 0.725

1.351 ± 0.261

7.729 ± 0.452

7.789 ± 1.614

10.491 ± 0.564

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.26

6.06 ± 0.876

3.636 ± 0.574

3.537 ± 0.269

3.437 ± 0.688

6.338 ± 0.6

6.179 ± 0.735

7.61 ± 0.48

1.848 ± 0.273

5.762 ± 0.691
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski