Ruminococcus sp. YE71

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; unclassified Ruminococcus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K1MQ30|A0A1K1MQ30_9FIRM D-alanyl-D-alanine carboxypeptidase OS=Ruminococcus sp. YE71 OX=244362 GN=SAMN02910447_01086 PE=4 SV=1
MM1 pKa = 7.91KK2 pKa = 9.79KK3 pKa = 10.41RR4 pKa = 11.84KK5 pKa = 8.04MVCVLAAVLLLAGCGKK21 pKa = 8.17TEE23 pKa = 4.24KK24 pKa = 10.09EE25 pKa = 3.98AASASAEE32 pKa = 4.05DD33 pKa = 3.93TAQASADD40 pKa = 3.7AADD43 pKa = 4.14GDD45 pKa = 4.57VVSQEE50 pKa = 4.29SAAEE54 pKa = 3.91PAPEE58 pKa = 4.15NADD61 pKa = 3.44SSSVTDD67 pKa = 3.24STSDD71 pKa = 3.55YY72 pKa = 10.28KK73 pKa = 10.81IKK75 pKa = 10.59SHH77 pKa = 6.93GSRR80 pKa = 11.84EE81 pKa = 4.21YY82 pKa = 11.2NGAAANDD89 pKa = 4.02DD90 pKa = 3.81FADD93 pKa = 3.64ALFIGDD99 pKa = 3.86SRR101 pKa = 11.84TVGLMLNGDD110 pKa = 4.22KK111 pKa = 10.89PKK113 pKa = 10.87ADD115 pKa = 3.83YY116 pKa = 9.97FATAGLNVMTAQSTPVVPKK135 pKa = 10.16RR136 pKa = 11.84DD137 pKa = 3.75FEE139 pKa = 4.33GLIVQQAYY147 pKa = 8.78TDD149 pKa = 4.06YY150 pKa = 11.47GWGDD154 pKa = 3.76NAWDD158 pKa = 3.65DD159 pKa = 4.25TTWDD163 pKa = 3.56DD164 pKa = 4.08TTWDD168 pKa = 3.62DD169 pKa = 4.87GGWTGGDD176 pKa = 3.25STEE179 pKa = 4.13WDD181 pKa = 3.2WGYY184 pKa = 11.15SEE186 pKa = 5.3PDD188 pKa = 3.2SSEE191 pKa = 3.88EE192 pKa = 3.97TSSVDD197 pKa = 3.3EE198 pKa = 4.91DD199 pKa = 4.66LMTIPEE205 pKa = 4.26ALALKK210 pKa = 9.43QYY212 pKa = 10.88KK213 pKa = 10.14RR214 pKa = 11.84IFINFGVNEE223 pKa = 4.83LGWPYY228 pKa = 10.63PDD230 pKa = 3.73VFVEE234 pKa = 4.96RR235 pKa = 11.84YY236 pKa = 9.07KK237 pKa = 11.23LLILQVRR244 pKa = 11.84AAQPDD249 pKa = 3.48AQIYY253 pKa = 8.65IEE255 pKa = 4.43SMLPVSPLALSIDD268 pKa = 3.26KK269 pKa = 10.77CYY271 pKa = 8.73TTEE274 pKa = 4.09NVDD277 pKa = 3.75NFNEE281 pKa = 4.26NYY283 pKa = 9.19VRR285 pKa = 11.84RR286 pKa = 11.84AAQEE290 pKa = 4.2TNCTYY295 pKa = 11.34LDD297 pKa = 3.56VNQFFRR303 pKa = 11.84DD304 pKa = 3.3ADD306 pKa = 3.67GGLFADD312 pKa = 4.76ASTDD316 pKa = 4.07GIHH319 pKa = 7.41LNRR322 pKa = 11.84DD323 pKa = 3.3YY324 pKa = 11.57CMEE327 pKa = 3.8WMDD330 pKa = 3.39ILSYY334 pKa = 9.3YY335 pKa = 9.03TVGGGDD341 pKa = 3.14ISILSRR347 pKa = 11.84RR348 pKa = 11.84HH349 pKa = 4.4EE350 pKa = 4.42TEE352 pKa = 3.7SAPDD356 pKa = 3.88GSIEE360 pKa = 4.14PYY362 pKa = 10.53SDD364 pKa = 3.69GGDD367 pKa = 3.61GYY369 pKa = 11.33SEE371 pKa = 5.61DD372 pKa = 5.52GGTDD376 pKa = 2.91WTDD379 pKa = 3.16GGDD382 pKa = 3.81NGWGGDD388 pKa = 4.61GYY390 pKa = 11.28SDD392 pKa = 3.38WNDD395 pKa = 2.93EE396 pKa = 4.28GGYY399 pKa = 10.92GDD401 pKa = 4.14YY402 pKa = 11.14NYY404 pKa = 10.79GYY406 pKa = 10.97EE407 pKa = 4.98DD408 pKa = 3.39YY409 pKa = 10.45TYY411 pKa = 11.22DD412 pKa = 3.93NEE414 pKa = 3.87YY415 pKa = 10.96DD416 pKa = 3.44YY417 pKa = 11.11DD418 pKa = 3.7YY419 pKa = 11.48GYY421 pKa = 11.75

Molecular weight: 46.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K1LMB9|A0A1K1LMB9_9FIRM Type IV pilus assembly protein PilB OS=Ruminococcus sp. YE71 OX=244362 GN=SAMN02910447_00223 PE=3 SV=1
MM1 pKa = 7.62TDD3 pKa = 2.38IKK5 pKa = 10.41TRR7 pKa = 11.84LQYY10 pKa = 10.51SAAVAAVILLGLVSRR25 pKa = 11.84RR26 pKa = 11.84IPVIPPCVGDD36 pKa = 4.1ALWAVVVFFCWRR48 pKa = 11.84IVLVKK53 pKa = 9.85KK54 pKa = 10.2HH55 pKa = 6.17PRR57 pKa = 11.84TAAAAALVTSFAVEE71 pKa = 4.31FSQLIRR77 pKa = 11.84WDD79 pKa = 2.97WLVRR83 pKa = 11.84VRR85 pKa = 11.84ATTIGHH91 pKa = 7.32LLLGQGFVWSDD102 pKa = 2.99LAAYY106 pKa = 9.76AVGIGAALLCVTAVGRR122 pKa = 11.84SKK124 pKa = 11.34

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3572

0

3572

1169459

39

3788

327.4

36.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.075 ± 0.045

1.689 ± 0.017

6.419 ± 0.035

7.156 ± 0.04

4.218 ± 0.029

7.194 ± 0.041

1.625 ± 0.015

6.785 ± 0.037

6.382 ± 0.034

8.41 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.02

4.236 ± 0.037

3.348 ± 0.021

2.609 ± 0.023

4.659 ± 0.036

6.639 ± 0.042

6.0 ± 0.042

6.712 ± 0.029

0.902 ± 0.015

4.134 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski