Pseudoxanthomonas sp. GM95

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Pseudoxanthomonas; unclassified Pseudoxanthomonas

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7XR37|A0A1H7XR37_9GAMM D-galactonate transporter OS=Pseudoxanthomonas sp. GM95 OX=1881043 GN=SAMN05428989_3636 PE=4 SV=1
MM1 pKa = 7.68PSSVMPSARR10 pKa = 11.84RR11 pKa = 11.84GRR13 pKa = 11.84TSSHH17 pKa = 6.97RR18 pKa = 11.84ILPALSCNPLTAALLLVLALPAAATAPASGNAAAAPATQASATGTATTQDD68 pKa = 3.95DD69 pKa = 4.59DD70 pKa = 4.22TTSVQPQSAEE80 pKa = 3.56AAASYY85 pKa = 9.34FAANGLGDD93 pKa = 4.02GSDD96 pKa = 3.96DD97 pKa = 3.6ATASGTNAVAVGAGADD113 pKa = 3.37ATGDD117 pKa = 3.5YY118 pKa = 10.66GVAIGSHH125 pKa = 5.24ATVTDD130 pKa = 3.97AYY132 pKa = 10.55GIAIGHH138 pKa = 6.21NVTAGNTAIAMGDD151 pKa = 3.54GAQATGQSAVAIGGSFFGSTYY172 pKa = 10.58GDD174 pKa = 3.15SDD176 pKa = 3.68GARR179 pKa = 11.84ATGFGAVSLGAYY191 pKa = 9.9AGATGSTSTALGWSSSAIEE210 pKa = 4.31SGTVAVGSSAHH221 pKa = 6.39AEE223 pKa = 4.17GVYY226 pKa = 10.32AAAFGSSTYY235 pKa = 10.13ATGTASTALGYY246 pKa = 8.64GTVASNDD253 pKa = 3.07ATVAIGTFGQADD265 pKa = 4.3GFAGVAVGYY274 pKa = 9.71YY275 pKa = 8.93ATASGDD281 pKa = 3.29GATALGEE288 pKa = 4.16SALAQGQQSVAIGASNAGVYY308 pKa = 7.82TQANGLGSVTVGAGSWALSDD328 pKa = 3.76YY329 pKa = 9.84GVAMGFDD336 pKa = 3.65SHH338 pKa = 8.07ADD340 pKa = 3.2AMYY343 pKa = 8.82ATALGAQAISTSTSATALGAQSFSDD368 pKa = 3.72GDD370 pKa = 3.44EE371 pKa = 4.09ATAVGYY377 pKa = 9.73IASASGLGASAFGAGASAFGDD398 pKa = 3.47GGLALGTNSAVIGANSVALGAGSFADD424 pKa = 3.77RR425 pKa = 11.84DD426 pKa = 3.84NTVSVGVAGAEE437 pKa = 3.65RR438 pKa = 11.84QVSNVAAGTEE448 pKa = 4.06DD449 pKa = 3.45TDD451 pKa = 4.0AVNVAQLKK459 pKa = 11.07SMTQNIGDD467 pKa = 4.15DD468 pKa = 3.84DD469 pKa = 6.02LYY471 pKa = 11.4FSANGAGDD479 pKa = 3.85GTDD482 pKa = 2.96TATASGTGSLAAGSSAEE499 pKa = 3.81ATGNYY504 pKa = 8.77GVALGSRR511 pKa = 11.84AVVSDD516 pKa = 4.14AYY518 pKa = 10.7GIAIGHH524 pKa = 5.85NVQAGNTAIAMGDD537 pKa = 3.54GAQATGQSAVAIGGSYY553 pKa = 10.18FGSAFGDD560 pKa = 3.69SIGAIGSGFGAVALGAAASATGSTSTAVGWAAHH593 pKa = 6.0TDD595 pKa = 4.02FSGGLAMGASAGAMADD611 pKa = 3.52GAVALGASSYY621 pKa = 8.06VTGIQGTGVGYY632 pKa = 9.88GAYY635 pKa = 8.7VTGTVGVALGGYY647 pKa = 8.78SEE649 pKa = 4.41VTGAFAMALGYY660 pKa = 9.87GAQTVGDD667 pKa = 3.65GGIAVGEE674 pKa = 3.97AALAQGQQSVAIGATNGGISTQANGLGSTTVGAGSWALSDD714 pKa = 3.67YY715 pKa = 10.87GVALGFDD722 pKa = 3.69SHH724 pKa = 8.08ADD726 pKa = 3.34AMYY729 pKa = 9.26STALGAQALSTSTSATALGASTFADD754 pKa = 3.61GDD756 pKa = 3.59EE757 pKa = 4.26ATAVGYY763 pKa = 9.67IASASGVGAAAFGAGASAFGDD784 pKa = 3.38GGLAVGYY791 pKa = 9.5NAAAVGLNSVALGAGSYY808 pKa = 10.98ADD810 pKa = 4.26RR811 pKa = 11.84DD812 pKa = 3.71NSVSFGVAGAEE823 pKa = 3.65RR824 pKa = 11.84QLTNIAAGTEE834 pKa = 4.01DD835 pKa = 3.64TDD837 pKa = 4.88AVNLSQLHH845 pKa = 6.15ALADD849 pKa = 3.9TLILSGGATVDD860 pKa = 3.9GMGEE864 pKa = 3.64MAAAFGGGAGYY875 pKa = 10.05SAAGGFVLPSYY886 pKa = 10.29SIQGGSYY893 pKa = 10.05TNVGDD898 pKa = 5.04ALTALDD904 pKa = 4.35GAFSGLSDD912 pKa = 3.83RR913 pKa = 11.84LASLEE918 pKa = 4.09TAPAAGSGAGGVAVGGGAADD938 pKa = 4.0GASVADD944 pKa = 4.22GSNGVAVGAGAQTGGEE960 pKa = 4.13NGTAVGGGAYY970 pKa = 10.11AAGPNDD976 pKa = 3.58TAIGGNAKK984 pKa = 10.01VNADD988 pKa = 2.9GSTAVGANTAIAAEE1002 pKa = 4.2ATNAVAVGEE1011 pKa = 4.35SASVSVASGTAVGQGASVTAEE1032 pKa = 3.88GAVALGQGSVADD1044 pKa = 4.17RR1045 pKa = 11.84ANTVSVGSTGSEE1057 pKa = 3.56RR1058 pKa = 11.84QVTHH1062 pKa = 6.01VAAGTAATDD1071 pKa = 3.62AANVGQMQAGDD1082 pKa = 3.89AQSVATANAYY1092 pKa = 8.63TDD1094 pKa = 3.54TTATRR1099 pKa = 11.84TLTSANSYY1107 pKa = 9.7TDD1109 pKa = 3.4QKK1111 pKa = 9.15LTAMDD1116 pKa = 4.3DD1117 pKa = 3.74RR1118 pKa = 11.84FTQLSNDD1125 pKa = 3.72VGHH1128 pKa = 7.35KK1129 pKa = 10.0LAAQDD1134 pKa = 3.92KK1135 pKa = 9.9RR1136 pKa = 11.84IDD1138 pKa = 3.35RR1139 pKa = 11.84MGAMGSAMMNMAINAANSKK1158 pKa = 8.55SAKK1161 pKa = 9.67GRR1163 pKa = 11.84IAAGAGWQNGEE1174 pKa = 3.93AALSVGYY1181 pKa = 10.28AKK1183 pKa = 10.6QIGEE1187 pKa = 4.15RR1188 pKa = 11.84ASFSIGGAFSGDD1200 pKa = 3.51DD1201 pKa = 3.3TSAGVGFGIDD1211 pKa = 3.25LL1212 pKa = 4.11

Molecular weight: 113.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7U7P7|A0A1H7U7P7_9GAMM GTPase Der OS=Pseudoxanthomonas sp. GM95 OX=1881043 GN=der PE=3 SV=1
MM1 pKa = 8.08ASTTQLKK8 pKa = 10.19IGAGALWIAGLATLVAALLLRR29 pKa = 11.84QPLLLPVGAVLIGVGRR45 pKa = 11.84TAMTRR50 pKa = 11.84ILRR53 pKa = 11.84RR54 pKa = 11.84TLPRR58 pKa = 4.93

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4071

0

4071

1401671

29

3738

344.3

37.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.056 ± 0.061

0.794 ± 0.01

5.884 ± 0.034

4.84 ± 0.033

3.382 ± 0.025

8.579 ± 0.035

2.207 ± 0.02

4.112 ± 0.027

2.918 ± 0.026

10.796 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.193 ± 0.018

2.566 ± 0.032

5.338 ± 0.03

4.103 ± 0.025

6.848 ± 0.043

5.444 ± 0.031

5.435 ± 0.039

7.524 ± 0.036

1.561 ± 0.018

2.422 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski