Wenling tombus-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH40|A0A1L3KH40_9VIRU Uncharacterized protein OS=Wenling tombus-like virus 2 OX=1923544 PE=4 SV=1
MM1 pKa = 6.77YY2 pKa = 10.8AEE4 pKa = 4.73IEE6 pKa = 4.34SAAWVYY12 pKa = 11.41NKK14 pKa = 10.85AKK16 pKa = 9.94FVWKK20 pKa = 9.25MYY22 pKa = 10.12PVLRR26 pKa = 11.84DD27 pKa = 3.39DD28 pKa = 3.7TFILYY33 pKa = 10.3RR34 pKa = 11.84SVEE37 pKa = 4.09LPKK40 pKa = 10.78LKK42 pKa = 10.63SFQTYY47 pKa = 10.64CNITGTISSSQYY59 pKa = 9.85YY60 pKa = 10.49GFDD63 pKa = 3.07EE64 pKa = 4.3VTLNLRR70 pKa = 11.84VEE72 pKa = 4.38RR73 pKa = 11.84SKK75 pKa = 10.9DD76 pKa = 2.98WKK78 pKa = 10.78VIEE81 pKa = 4.25EE82 pKa = 4.37SMVTLDD88 pKa = 3.26SHH90 pKa = 6.49TPGNLFPTDD99 pKa = 3.12NRR101 pKa = 11.84SAVQLLYY108 pKa = 10.41TGTFEE113 pKa = 5.01VPPDD117 pKa = 3.36HH118 pKa = 7.26HH119 pKa = 6.71ITTSSILAVIARR131 pKa = 11.84YY132 pKa = 8.51PVVATTTLSLAGRR145 pKa = 11.84SAWDD149 pKa = 3.28ILRR152 pKa = 11.84MYY154 pKa = 9.91FKK156 pKa = 11.14VSFHH160 pKa = 6.82SSLVFNIKK168 pKa = 10.46GEE170 pKa = 4.15EE171 pKa = 3.92NIKK174 pKa = 10.06IVRR177 pKa = 11.84SASFPMLSHH186 pKa = 7.12RR187 pKa = 11.84SLASSSSWQSLPEE200 pKa = 4.07LSSGRR205 pKa = 11.84SSAQLSSVSLNSEE218 pKa = 3.5EE219 pKa = 4.31WIAII223 pKa = 3.82

Molecular weight: 25.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGP2|A0A1L3KGP2_9VIRU Uncharacterized protein OS=Wenling tombus-like virus 2 OX=1923544 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 4.88IVPRR6 pKa = 11.84GRR8 pKa = 11.84RR9 pKa = 11.84APPLGTVQAALRR21 pKa = 11.84GLNLARR27 pKa = 11.84QAYY30 pKa = 9.32NWMNPAPQQPQNPPGTGRR48 pKa = 11.84RR49 pKa = 11.84SRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84GGRR56 pKa = 11.84GRR58 pKa = 11.84GVSTPVQPIPMPIYY72 pKa = 9.23PAFPRR77 pKa = 11.84GRR79 pKa = 11.84GRR81 pKa = 11.84GRR83 pKa = 11.84GRR85 pKa = 11.84GRR87 pKa = 11.84VLTTNPGRR95 pKa = 11.84SVTITQSEE103 pKa = 4.55VWGDD107 pKa = 3.62VVKK110 pKa = 10.97GPNVFTFQPSAIGMPQLKK128 pKa = 9.66HH129 pKa = 5.98LAAGYY134 pKa = 7.93TRR136 pKa = 11.84YY137 pKa = 10.32KK138 pKa = 9.82VIRR141 pKa = 11.84VTLEE145 pKa = 3.38YY146 pKa = 9.98EE147 pKa = 4.05PAVGRR152 pKa = 11.84QRR154 pKa = 11.84DD155 pKa = 4.15GVIHH159 pKa = 6.04WGIRR163 pKa = 11.84PGVVAKK169 pKa = 9.41ATATPEE175 pKa = 4.14VCKK178 pKa = 10.09AAYY181 pKa = 8.2PRR183 pKa = 11.84RR184 pKa = 11.84SHH186 pKa = 6.16PVYY189 pKa = 10.78VRR191 pKa = 11.84TTMTIGHH198 pKa = 7.26DD199 pKa = 4.71LIMMQPWLGTGEE211 pKa = 4.13TAFTLVANSPGDD223 pKa = 3.47TTQPGFFEE231 pKa = 4.36CTYY234 pKa = 10.77HH235 pKa = 7.03VVLDD239 pKa = 4.18LPKK242 pKa = 10.49PPP244 pKa = 4.46

Molecular weight: 26.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1276

223

443

319.0

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.661 ± 0.214

1.019 ± 0.166

4.624 ± 0.834

5.721 ± 1.026

3.448 ± 0.273

5.721 ± 1.454

2.665 ± 0.223

5.486 ± 0.38

6.27 ± 1.366

7.915 ± 0.775

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.056 ± 0.211

3.213 ± 0.101

6.505 ± 1.373

4.389 ± 0.775

6.426 ± 1.188

7.602 ± 1.6

6.113 ± 0.762

7.132 ± 0.751

1.803 ± 0.189

4.232 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski