Klebsiella phage 5 LV-2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2F9D3|A0A2Z2F9D3_9CAUD Uncharacterized protein OS=Klebsiella phage 5 LV-2017 OX=1960659 PE=4 SV=1
MM1 pKa = 7.6TDD3 pKa = 2.82ITEE6 pKa = 4.39LAQRR10 pKa = 11.84NEE12 pKa = 3.85LLIANGQQTADD23 pKa = 4.2LLRR26 pKa = 11.84HH27 pKa = 5.72LADD30 pKa = 3.88NEE32 pKa = 3.75IDD34 pKa = 3.3SDD36 pKa = 4.19YY37 pKa = 10.83FAVVSEE43 pKa = 4.36CEE45 pKa = 4.36SYY47 pKa = 11.27GKK49 pKa = 8.46EE50 pKa = 3.68TDD52 pKa = 3.53AEE54 pKa = 4.12LSITEE59 pKa = 4.14FALRR63 pKa = 11.84AAGYY67 pKa = 10.57VDD69 pKa = 4.13ALVEE73 pKa = 4.22ALEE76 pKa = 4.32KK77 pKa = 10.94AQTINAAAEE86 pKa = 3.92KK87 pKa = 10.31LVRR90 pKa = 11.84CKK92 pKa = 10.64GRR94 pKa = 11.84YY95 pKa = 9.0HH96 pKa = 7.41SEE98 pKa = 3.54QNYY101 pKa = 9.45RR102 pKa = 11.84ALAALFGVNTPDD114 pKa = 5.39LPPLDD119 pKa = 4.95DD120 pKa = 4.21EE121 pKa = 4.72SHH123 pKa = 6.33AVTVEE128 pKa = 3.82NQQEE132 pKa = 4.16SAYY135 pKa = 10.47RR136 pKa = 11.84AGLTAGWNLGLANNNDD152 pKa = 3.42GFNACLAAHH161 pKa = 6.17TAGIKK166 pKa = 10.23VEE168 pKa = 4.25AEE170 pKa = 3.83

Molecular weight: 18.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2FDP0|A0A2Z2FDP0_9CAUD Uncharacterized protein OS=Klebsiella phage 5 LV-2017 OX=1960659 PE=4 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84ALSAVEE9 pKa = 3.28RR10 pKa = 11.84RR11 pKa = 11.84EE12 pKa = 3.67YY13 pKa = 10.04VRR15 pKa = 11.84AVIRR19 pKa = 11.84ITRR22 pKa = 11.84HH23 pKa = 4.06QGRR26 pKa = 11.84LTTTEE31 pKa = 3.68AMKK34 pKa = 10.94KK35 pKa = 10.03LGLSRR40 pKa = 11.84ATVQRR45 pKa = 11.84YY46 pKa = 7.82FSEE49 pKa = 5.16AEE51 pKa = 3.76ATGEE55 pKa = 4.31VVRR58 pKa = 11.84HH59 pKa = 5.21GRR61 pKa = 11.84LGLFRR66 pKa = 11.84DD67 pKa = 3.57QRR69 pKa = 11.84AVIDD73 pKa = 3.78FDD75 pKa = 3.63MKK77 pKa = 10.91RR78 pKa = 11.84FGLVPKK84 pKa = 10.23VAVGMNYY91 pKa = 10.4SLLGSPVFQRR101 pKa = 11.84VLDD104 pKa = 3.85VQEE107 pKa = 5.21AIHH110 pKa = 6.31GG111 pKa = 4.0

Molecular weight: 12.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

11976

85

1213

292.1

32.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.822 ± 0.527

1.06 ± 0.2

5.995 ± 0.284

6.755 ± 0.392

3.064 ± 0.187

7.181 ± 0.366

1.62 ± 0.181

5.085 ± 0.185

5.528 ± 0.465

8.308 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.363 ± 0.133

3.991 ± 0.217

4.284 ± 0.279

5.085 ± 0.582

5.995 ± 0.408

5.995 ± 0.395

6.279 ± 0.485

6.187 ± 0.403

1.528 ± 0.163

2.872 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski