Lysobacter lacus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2756 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D8YJ53|A0A5D8YJ53_9GAMM Uncharacterized protein OS=Lysobacter lacus OX=1643323 GN=FW784_13270 PE=4 SV=1
MM1 pKa = 6.78STAAEE6 pKa = 4.04TAPFRR11 pKa = 11.84TWMCVVCGYY20 pKa = 10.36IYY22 pKa = 10.75DD23 pKa = 3.97EE24 pKa = 4.54AKK26 pKa = 10.44GLPEE30 pKa = 4.1EE31 pKa = 5.42GIAPGTRR38 pKa = 11.84WADD41 pKa = 3.54VPDD44 pKa = 3.6TWTCPDD50 pKa = 3.91CGVTKK55 pKa = 10.7DD56 pKa = 3.63DD57 pKa = 4.28FEE59 pKa = 4.4MVEE62 pKa = 3.98AA63 pKa = 5.12

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D8ZB82|A0A5D8ZB82_9GAMM rRNA methyltransferase OS=Lysobacter lacus OX=1643323 GN=FW784_07265 PE=4 SV=1
MM1 pKa = 7.05ATKK4 pKa = 10.45KK5 pKa = 9.28ATKK8 pKa = 9.97KK9 pKa = 10.71SPAKK13 pKa = 10.35KK14 pKa = 8.76ATKK17 pKa = 9.86KK18 pKa = 10.58AAGKK22 pKa = 9.79KK23 pKa = 9.36AATRR27 pKa = 11.84KK28 pKa = 9.68AATKK32 pKa = 8.97KK33 pKa = 8.54TGARR37 pKa = 11.84KK38 pKa = 7.33TAAKK42 pKa = 10.19KK43 pKa = 9.81VGARR47 pKa = 11.84KK48 pKa = 8.26ATAKK52 pKa = 10.46KK53 pKa = 8.48STARR57 pKa = 11.84KK58 pKa = 6.84TAAKK62 pKa = 10.11KK63 pKa = 10.14GGARR67 pKa = 11.84KK68 pKa = 7.38TAAKK72 pKa = 10.27KK73 pKa = 10.26GGARR77 pKa = 11.84KK78 pKa = 8.21ATAKK82 pKa = 10.52KK83 pKa = 8.58STARR87 pKa = 11.84KK88 pKa = 7.98ATAKK92 pKa = 10.38KK93 pKa = 8.48STARR97 pKa = 11.84KK98 pKa = 6.84TAAKK102 pKa = 10.11KK103 pKa = 10.14GGARR107 pKa = 11.84KK108 pKa = 7.38TAAKK112 pKa = 10.27KK113 pKa = 10.26GGARR117 pKa = 11.84KK118 pKa = 8.21ATAKK122 pKa = 10.52KK123 pKa = 8.58STARR127 pKa = 11.84KK128 pKa = 7.98ATAKK132 pKa = 10.38KK133 pKa = 8.48STARR137 pKa = 11.84KK138 pKa = 6.84TAAKK142 pKa = 10.11KK143 pKa = 10.14GGARR147 pKa = 11.84KK148 pKa = 7.38TAAKK152 pKa = 10.27KK153 pKa = 10.26GGARR157 pKa = 11.84KK158 pKa = 8.21ATAKK162 pKa = 10.46KK163 pKa = 8.48STARR167 pKa = 11.84KK168 pKa = 6.84TAAKK172 pKa = 10.21KK173 pKa = 10.26GGARR177 pKa = 11.84KK178 pKa = 8.22ATAKK182 pKa = 10.38KK183 pKa = 10.48AGGARR188 pKa = 11.84KK189 pKa = 8.19ATAKK193 pKa = 10.52KK194 pKa = 10.21AGARR198 pKa = 11.84KK199 pKa = 9.66APAKK203 pKa = 10.12KK204 pKa = 10.32AGGARR209 pKa = 11.84KK210 pKa = 8.24STAKK214 pKa = 10.37KK215 pKa = 10.24AGGARR220 pKa = 11.84KK221 pKa = 6.33MTRR224 pKa = 11.84RR225 pKa = 11.84SGRR228 pKa = 11.84GSDD231 pKa = 3.68ALANMPATPAPMII244 pKa = 4.39

Molecular weight: 24.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2756

0

2756

837149

30

1923

303.8

32.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.29 ± 0.064

0.86 ± 0.016

6.105 ± 0.041

5.128 ± 0.046

3.381 ± 0.028

8.584 ± 0.045

2.338 ± 0.022

4.109 ± 0.031

2.529 ± 0.039

10.374 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.022

2.334 ± 0.031

5.348 ± 0.039

3.323 ± 0.029

8.149 ± 0.055

5.217 ± 0.033

5.045 ± 0.036

7.947 ± 0.044

1.421 ± 0.022

2.221 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski