Wild carrot mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus; unclassified Umbravirus

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H2MW55|A0A6H2MW55_9TOMB RNA-directed RNA polymerase OS=Wild carrot mottle virus OX=1862686 PE=4 SV=1
MM1 pKa = 7.52SILRR5 pKa = 11.84KK6 pKa = 9.56VVQFLDD12 pKa = 3.79AKK14 pKa = 10.19YY15 pKa = 10.46SPAPGILSRR24 pKa = 11.84EE25 pKa = 4.09QCAEE29 pKa = 3.8RR30 pKa = 11.84YY31 pKa = 8.56GADD34 pKa = 2.8WAVLVTQEE42 pKa = 3.95RR43 pKa = 11.84MTRR46 pKa = 11.84ALSEE50 pKa = 4.04EE51 pKa = 3.93HH52 pKa = 6.45EE53 pKa = 4.24RR54 pKa = 11.84WYY56 pKa = 11.07SCNAEE61 pKa = 4.19TNVYY65 pKa = 10.09IPVTPYY71 pKa = 10.14VDD73 pKa = 3.48PRR75 pKa = 11.84DD76 pKa = 3.7VVAPLIEE83 pKa = 4.96DD84 pKa = 3.68AASTGDD90 pKa = 3.69AAPEE94 pKa = 3.96SDD96 pKa = 4.57AGEE99 pKa = 4.34PDD101 pKa = 3.17AAQLVGEE108 pKa = 4.65VVASRR113 pKa = 11.84PATGPGPTPYY123 pKa = 10.35SSEE126 pKa = 4.15EE127 pKa = 4.22LEE129 pKa = 4.51SATPYY134 pKa = 10.43CFTVEE139 pKa = 4.11AVGHH143 pKa = 6.67IIPVGRR149 pKa = 11.84MEE151 pKa = 4.34GTSVTRR157 pKa = 11.84EE158 pKa = 3.6GDD160 pKa = 3.4TAEE163 pKa = 4.67SDD165 pKa = 4.21SEE167 pKa = 4.36TDD169 pKa = 3.04TCSPAFYY176 pKa = 9.91IGAGNAVPVTAANGEE191 pKa = 4.14EE192 pKa = 4.53GQPQTATPIPGEE204 pKa = 4.22VPAFRR209 pKa = 11.84AEE211 pKa = 4.23DD212 pKa = 3.71PTISRR217 pKa = 11.84VPTTIVTGDD226 pKa = 3.42AAEE229 pKa = 4.25AMPTKK234 pKa = 10.57VDD236 pKa = 3.64DD237 pKa = 4.29CDD239 pKa = 4.75GSVSSSLMKK248 pKa = 10.63AGPTMAAMDD257 pKa = 4.29SYY259 pKa = 11.09SVAMEE264 pKa = 3.74LRR266 pKa = 11.84ARR268 pKa = 11.84FGLRR272 pKa = 11.84SKK274 pKa = 9.8TPANAEE280 pKa = 3.64LGGRR284 pKa = 11.84VSRR287 pKa = 11.84EE288 pKa = 3.8ILSHH292 pKa = 6.09CGGVRR297 pKa = 11.84SEE299 pKa = 4.29VYY301 pKa = 10.62YY302 pKa = 10.65LAQLAVQLWFAPTLLDD318 pKa = 4.9LVLTHH323 pKa = 6.04QVKK326 pKa = 10.6DD327 pKa = 3.86FCC329 pKa = 6.6

Molecular weight: 35.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H2MW43|A0A6H2MW43_9TOMB Cell to Cell MP OS=Wild carrot mottle virus OX=1862686 PE=4 SV=1
MM1 pKa = 7.63SGPGTPGFCPLWARR15 pKa = 11.84QVAHH19 pKa = 6.39TLSCKK24 pKa = 10.45LGALVIGIAALVSSGGACPQYY45 pKa = 10.81SHH47 pKa = 7.72PSGSRR52 pKa = 11.84QCRR55 pKa = 11.84EE56 pKa = 4.05GNSMTFRR63 pKa = 11.84LIYY66 pKa = 10.43DD67 pKa = 4.65GIEE70 pKa = 3.76QNSINNRR77 pKa = 11.84RR78 pKa = 11.84TNQPVTWRR86 pKa = 11.84SYY88 pKa = 10.33SGRR91 pKa = 11.84AQGARR96 pKa = 11.84PRR98 pKa = 11.84SYY100 pKa = 9.75DD101 pKa = 3.26TSPRR105 pKa = 11.84EE106 pKa = 3.97QYY108 pKa = 10.69GYY110 pKa = 9.31PHH112 pKa = 7.7AIIPAPPPAHH122 pKa = 5.87YY123 pKa = 10.29QPIPYY128 pKa = 8.75WPLAYY133 pKa = 7.61EE134 pKa = 4.17THH136 pKa = 6.63GGNAVYY142 pKa = 10.34RR143 pKa = 11.84EE144 pKa = 4.36VGGSVYY150 pKa = 10.47SPRR153 pKa = 11.84ARR155 pKa = 11.84HH156 pKa = 5.14GRR158 pKa = 11.84GRR160 pKa = 11.84GNYY163 pKa = 8.08MGARR167 pKa = 11.84HH168 pKa = 5.77SSSRR172 pKa = 11.84TGANRR177 pKa = 11.84GEE179 pKa = 4.29PEE181 pKa = 4.08SLHH184 pKa = 5.59TFVFRR189 pKa = 11.84TPAGRR194 pKa = 11.84FLSEE198 pKa = 4.34LFNSVEE204 pKa = 4.07RR205 pKa = 11.84LSRR208 pKa = 11.84EE209 pKa = 4.24CPSLLQSSDD218 pKa = 3.42PVGGGSTGARR228 pKa = 11.84GEE230 pKa = 4.11RR231 pKa = 11.84LLAVLNVAANNRR243 pKa = 11.84GEE245 pKa = 4.32GAVLQANTTGGCGNPEE261 pKa = 4.0AQHH264 pKa = 5.84QVTTPRR270 pKa = 11.84PNPTAAIPEE279 pKa = 4.12CRR281 pKa = 11.84IDD283 pKa = 3.3EE284 pKa = 4.36RR285 pKa = 11.84GRR287 pKa = 11.84DD288 pKa = 3.78GEE290 pKa = 4.43GTHH293 pKa = 6.42TKK295 pKa = 10.36LAVPHH300 pKa = 6.12PHH302 pKa = 6.18LCGRR306 pKa = 11.84VHH308 pKa = 7.96SEE310 pKa = 4.03GEE312 pKa = 4.08PCQGEE317 pKa = 4.1PTVRR321 pKa = 11.84YY322 pKa = 9.25LL323 pKa = 4.3

Molecular weight: 34.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1779

253

874

444.8

48.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.162 ± 0.8

2.024 ± 0.255

4.609 ± 1.008

6.352 ± 0.542

2.923 ± 0.609

8.094 ± 1.033

2.361 ± 0.376

4.16 ± 0.45

3.316 ± 1.077

7.532 ± 0.716

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.43

2.923 ± 0.416

7.701 ± 0.559

3.373 ± 0.274

6.633 ± 0.862

7.195 ± 0.513

6.633 ± 0.851

7.87 ± 0.667

1.124 ± 0.095

3.766 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski