Pseudomonad phage gh-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ghunavirus; Pseudomonas virus gh1

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q859E8|Q859E8_9CAUD Major capsid protein OS=Pseudomonad phage gh-1 OX=197783 GN=10 PE=3 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84SYY4 pKa = 11.02EE5 pKa = 4.16ATLEE9 pKa = 4.15TDD11 pKa = 4.33DD12 pKa = 4.81EE13 pKa = 4.33LAAINDD19 pKa = 3.49MLAAIGEE26 pKa = 4.66SPVSSLEE33 pKa = 3.75GDD35 pKa = 3.85PNADD39 pKa = 2.75VANARR44 pKa = 11.84RR45 pKa = 11.84ILNQVNRR52 pKa = 11.84EE53 pKa = 4.03VQSRR57 pKa = 11.84GWTFNIEE64 pKa = 3.87EE65 pKa = 4.53GAVLSPDD72 pKa = 3.5SFSGLIEE79 pKa = 4.01YY80 pKa = 10.61LSDD83 pKa = 3.37YY84 pKa = 11.19LRR86 pKa = 11.84ITTSGGTVYY95 pKa = 10.65VNRR98 pKa = 11.84GGYY101 pKa = 9.92VYY103 pKa = 10.76DD104 pKa = 4.48RR105 pKa = 11.84STKK108 pKa = 9.41TDD110 pKa = 2.9VYY112 pKa = 10.74TNDD115 pKa = 2.79ITVDD119 pKa = 4.39LIRR122 pKa = 11.84FKK124 pKa = 10.5TFSEE128 pKa = 4.23MPEE131 pKa = 4.1CFRR134 pKa = 11.84SYY136 pKa = 10.38IVAKK140 pKa = 10.26ASRR143 pKa = 11.84RR144 pKa = 11.84FNIRR148 pKa = 11.84FFGAGEE154 pKa = 4.11IEE156 pKa = 4.68GSLQEE161 pKa = 4.25QEE163 pKa = 4.11SEE165 pKa = 3.75AWQQCQEE172 pKa = 4.14YY173 pKa = 9.05EE174 pKa = 3.73LDD176 pKa = 3.55YY177 pKa = 11.63GGFNMIDD184 pKa = 3.33GDD186 pKa = 4.42SYY188 pKa = 11.67VGGIASRR195 pKa = 3.65

Molecular weight: 21.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q859G3|Q859G3_9CAUD Uncharacterized protein OS=Pseudomonad phage gh-1 OX=197783 PE=4 SV=1
MM1 pKa = 7.66SSPKK5 pKa = 10.39SYY7 pKa = 10.88LQTTSGRR14 pKa = 11.84VHH16 pKa = 5.64VKK18 pKa = 9.1VWEE21 pKa = 4.21EE22 pKa = 3.44AHH24 pKa = 6.57GPVPKK29 pKa = 10.43GMHH32 pKa = 5.44VDD34 pKa = 3.27HH35 pKa = 7.3RR36 pKa = 11.84NGDD39 pKa = 3.04IHH41 pKa = 7.71DD42 pKa = 4.1NRR44 pKa = 11.84LEE46 pKa = 3.94NLRR49 pKa = 11.84LATPAQNIANSKK61 pKa = 10.09LSEE64 pKa = 4.07ANKK67 pKa = 9.72TGLKK71 pKa = 9.8GLSWDD76 pKa = 3.6SARR79 pKa = 11.84GRR81 pKa = 11.84FKK83 pKa = 11.04GAVRR87 pKa = 11.84LNRR90 pKa = 11.84KK91 pKa = 9.13QYY93 pKa = 6.05TTRR96 pKa = 11.84GDD98 pKa = 4.27LLTVAAWLFRR108 pKa = 11.84TRR110 pKa = 11.84EE111 pKa = 4.01ALHH114 pKa = 6.68GEE116 pKa = 4.87FARR119 pKa = 11.84HH120 pKa = 5.1RR121 pKa = 3.94

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

11815

25

1392

281.3

31.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.928 ± 0.513

0.872 ± 0.164

6.466 ± 0.282

6.737 ± 0.449

3.597 ± 0.219

8.066 ± 0.308

1.913 ± 0.203

5.078 ± 0.22

5.781 ± 0.358

7.922 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.166

4.139 ± 0.33

3.775 ± 0.242

4.537 ± 0.3

5.857 ± 0.202

6.289 ± 0.434

5.417 ± 0.329

6.526 ± 0.298

1.473 ± 0.156

3.005 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski