Halodesulfurarchaeum formicicum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halodesulfurarchaeum

Average proteome isoelectric point is 4.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8S6A5|A0A1D8S6A5_9EURY Molybdopterin oxidoreductase OS=Halodesulfurarchaeum formicicum OX=1873524 GN=HSR6_1768 PE=4 SV=1
MM1 pKa = 7.22EE2 pKa = 4.91RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84FIASSVAVGLSLLAGCSSDD24 pKa = 3.89QEE26 pKa = 4.36PSNGTTTTTPQATTTAADD44 pKa = 3.89GSNGDD49 pKa = 3.48EE50 pKa = 4.03TVQFGAVYY58 pKa = 10.22QLEE61 pKa = 4.02SDD63 pKa = 4.07YY64 pKa = 11.68AVDD67 pKa = 3.29IQYY70 pKa = 11.11DD71 pKa = 3.7DD72 pKa = 4.31PEE74 pKa = 6.16RR75 pKa = 11.84GLTGSATARR84 pKa = 11.84FEE86 pKa = 4.32GDD88 pKa = 3.52DD89 pKa = 3.69YY90 pKa = 11.1YY91 pKa = 11.68QRR93 pKa = 11.84IEE95 pKa = 4.29DD96 pKa = 3.8SSTGDD101 pKa = 3.18VYY103 pKa = 11.36EE104 pKa = 4.95LYY106 pKa = 10.66HH107 pKa = 8.29VDD109 pKa = 3.04GTDD112 pKa = 3.26YY113 pKa = 11.54VVINDD118 pKa = 3.61EE119 pKa = 4.44TCIKK123 pKa = 10.81NPGPSVEE130 pKa = 4.35PEE132 pKa = 3.83EE133 pKa = 5.31SDD135 pKa = 3.5VDD137 pKa = 4.15TEE139 pKa = 4.26ADD141 pKa = 3.54TYY143 pKa = 11.57AEE145 pKa = 5.04KK146 pKa = 10.54PDD148 pKa = 3.99ADD150 pKa = 3.93LTAKK154 pKa = 8.96GTTTIDD160 pKa = 3.32GEE162 pKa = 4.58TVYY165 pKa = 10.76VFEE168 pKa = 4.48VTGEE172 pKa = 4.17DD173 pKa = 3.14VDD175 pKa = 4.1GTLTIYY181 pKa = 10.56VSQATGYY188 pKa = 9.71LRR190 pKa = 11.84RR191 pKa = 11.84VEE193 pKa = 4.39SDD195 pKa = 2.33WGRR198 pKa = 11.84LDD200 pKa = 3.33FHH202 pKa = 7.6SWGDD206 pKa = 3.68VEE208 pKa = 6.02PITAPDD214 pKa = 3.83MEE216 pKa = 4.93CQDD219 pKa = 3.97FSNN222 pKa = 4.11

Molecular weight: 24.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8S516|A0A1D8S516_9EURY MATE family drug/sodium antiporter OS=Halodesulfurarchaeum formicicum OX=1873524 GN=HSR6_1339 PE=4 SV=1
MM1 pKa = 7.8SIPAEE6 pKa = 4.23RR7 pKa = 11.84IEE9 pKa = 4.26RR10 pKa = 11.84LTDD13 pKa = 3.63LAHH16 pKa = 6.99AAAKK20 pKa = 9.82ADD22 pKa = 3.59RR23 pKa = 11.84MDD25 pKa = 3.45RR26 pKa = 11.84ARR28 pKa = 11.84RR29 pKa = 11.84YY30 pKa = 9.13VRR32 pKa = 11.84LARR35 pKa = 11.84RR36 pKa = 11.84IAEE39 pKa = 4.31RR40 pKa = 11.84NRR42 pKa = 11.84LSLPTRR48 pKa = 11.84LDD50 pKa = 3.17RR51 pKa = 11.84FTCDD55 pKa = 2.58ACDD58 pKa = 3.52AYY60 pKa = 11.09LIPGRR65 pKa = 11.84NARR68 pKa = 11.84VRR70 pKa = 11.84TQSGHH75 pKa = 5.19VVLTCSCGAQKK86 pKa = 10.4RR87 pKa = 11.84YY88 pKa = 9.57PYY90 pKa = 11.13

Molecular weight: 10.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2099

0

2099

623574

42

2576

297.1

32.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.849 ± 0.076

0.534 ± 0.018

7.543 ± 0.057

9.01 ± 0.081

3.253 ± 0.033

8.473 ± 0.057

1.96 ± 0.024

4.732 ± 0.037

1.93 ± 0.027

9.115 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.829 ± 0.021

2.307 ± 0.043

4.85 ± 0.039

2.859 ± 0.029

6.29 ± 0.06

5.476 ± 0.045

6.724 ± 0.05

8.685 ± 0.053

1.05 ± 0.019

2.532 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski