Hubei tombus-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGE9|A0A1L3KGE9_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 4 OX=1923288 PE=4 SV=1
MM1 pKa = 7.51GKK3 pKa = 9.25QRR5 pKa = 11.84AVTQIGRR12 pKa = 11.84VAPPNRR18 pKa = 11.84AAVHH22 pKa = 6.53DD23 pKa = 4.31NSVLNCDD30 pKa = 2.97RR31 pKa = 11.84ALRR34 pKa = 11.84EE35 pKa = 3.28RR36 pKa = 11.84VYY38 pKa = 10.76YY39 pKa = 10.12VKK41 pKa = 10.97NGDD44 pKa = 3.4GFARR48 pKa = 11.84PPQPNPSSVLTLGQFRR64 pKa = 11.84KK65 pKa = 10.02KK66 pKa = 10.51LSRR69 pKa = 11.84CVPRR73 pKa = 11.84IPALSWEE80 pKa = 4.66LFPSRR85 pKa = 11.84YY86 pKa = 9.69KK87 pKa = 10.46DD88 pKa = 3.09AKK90 pKa = 9.95KK91 pKa = 10.73RR92 pKa = 11.84NIYY95 pKa = 10.4KK96 pKa = 10.5NADD99 pKa = 3.27QSLHH103 pKa = 5.69EE104 pKa = 4.96RR105 pKa = 11.84PLSIHH110 pKa = 6.87DD111 pKa = 4.13SYY113 pKa = 12.26VKK115 pKa = 10.79GFIKK119 pKa = 10.39DD120 pKa = 3.46EE121 pKa = 4.85KK122 pKa = 10.02INLMKK127 pKa = 10.64KK128 pKa = 9.43EE129 pKa = 4.2DD130 pKa = 3.92PVPRR134 pKa = 11.84AILPLSPRR142 pKa = 11.84LNIVEE147 pKa = 4.11GSIISHH153 pKa = 6.51RR154 pKa = 11.84EE155 pKa = 3.38HH156 pKa = 7.3AVFEE160 pKa = 4.96GIDD163 pKa = 3.43LVFGRR168 pKa = 11.84KK169 pKa = 7.25TVMKK173 pKa = 10.23GLNAEE178 pKa = 4.03EE179 pKa = 4.36KK180 pKa = 10.86GRR182 pKa = 11.84IIAEE186 pKa = 3.87KK187 pKa = 9.91CGRR190 pKa = 11.84FAKK193 pKa = 10.51FAALSIDD200 pKa = 3.35MAKK203 pKa = 10.13FDD205 pKa = 3.57QHH207 pKa = 8.33VSVLMNMFEE216 pKa = 4.16NSVLVDD222 pKa = 3.62TCCTPQEE229 pKa = 3.92RR230 pKa = 11.84RR231 pKa = 11.84EE232 pKa = 3.99LSKK235 pKa = 10.6ILAWSIHH242 pKa = 5.55SKK244 pKa = 10.93GRR246 pKa = 11.84MQATDD251 pKa = 3.18GKK253 pKa = 11.01VNFIRR258 pKa = 11.84EE259 pKa = 4.28GGRR262 pKa = 11.84LSGCMHH268 pKa = 7.07TSLGNIIIMCAMFWTFMQQFGDD290 pKa = 3.93IKK292 pKa = 10.12WDD294 pKa = 3.91YY295 pKa = 11.37INDD298 pKa = 3.68GDD300 pKa = 4.22DD301 pKa = 3.21CVLFVEE307 pKa = 5.79DD308 pKa = 4.84KK309 pKa = 10.65DD310 pKa = 3.87LHH312 pKa = 6.17KK313 pKa = 10.66VAPKK317 pKa = 9.89IQDD320 pKa = 3.3YY321 pKa = 9.86FLGMGFTAVVEE332 pKa = 4.41DD333 pKa = 4.03VVHH336 pKa = 7.27DD337 pKa = 3.51IEE339 pKa = 7.11KK340 pKa = 10.11IDD342 pKa = 3.88FCQSRR347 pKa = 11.84PVWDD351 pKa = 3.96GDD353 pKa = 3.9DD354 pKa = 4.81YY355 pKa = 11.77IMCRR359 pKa = 11.84NPHH362 pKa = 5.4TALIKK367 pKa = 10.24DD368 pKa = 4.07TMCLKK373 pKa = 10.73PIGNEE378 pKa = 4.03NEE380 pKa = 3.58WGSWVRR386 pKa = 11.84SVSEE390 pKa = 3.81SGIAGFAGMPIFQSFYY406 pKa = 9.21EE407 pKa = 4.32LYY409 pKa = 10.3SRR411 pKa = 11.84SSKK414 pKa = 10.28GFKK417 pKa = 9.74VNKK420 pKa = 8.9LHH422 pKa = 7.38RR423 pKa = 11.84VDD425 pKa = 4.45GGLKK429 pKa = 8.67IATRR433 pKa = 11.84GMHH436 pKa = 6.02RR437 pKa = 11.84KK438 pKa = 8.97SRR440 pKa = 11.84PIDD443 pKa = 3.38DD444 pKa = 3.53RR445 pKa = 11.84TRR447 pKa = 11.84YY448 pKa = 10.19SFWLAWGVLPDD459 pKa = 3.76AQICTEE465 pKa = 4.0EE466 pKa = 4.48MFSTMHH472 pKa = 6.71LNYY475 pKa = 9.4HH476 pKa = 6.53DD477 pKa = 4.56PVLSFEE483 pKa = 4.43SLSLPSYY490 pKa = 10.25FF491 pKa = 4.71

Molecular weight: 56.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGE9|A0A1L3KGE9_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 4 OX=1923288 PE=4 SV=1
MM1 pKa = 7.47ATTLLIPAYY10 pKa = 9.56IATIHH15 pKa = 6.18AADD18 pKa = 4.27YY19 pKa = 9.55AWSVYY24 pKa = 9.26TSSTNPNDD32 pKa = 3.52DD33 pKa = 3.35SFEE36 pKa = 4.39RR37 pKa = 11.84RR38 pKa = 11.84VEE40 pKa = 3.97WNRR43 pKa = 11.84VKK45 pKa = 10.45SWFRR49 pKa = 11.84GTTALSRR56 pKa = 11.84EE57 pKa = 4.34DD58 pKa = 3.34EE59 pKa = 4.25RR60 pKa = 11.84RR61 pKa = 11.84GHH63 pKa = 5.94NMLRR67 pKa = 11.84EE68 pKa = 4.21FNSEE72 pKa = 3.91EE73 pKa = 4.37SGDD76 pKa = 3.82LPTFEE81 pKa = 6.61DD82 pKa = 3.71EE83 pKa = 5.43DD84 pKa = 4.6LFDD87 pKa = 4.58VEE89 pKa = 5.65LDD91 pKa = 3.76GEE93 pKa = 4.79GKK95 pKa = 8.1VTRR98 pKa = 11.84KK99 pKa = 9.55KK100 pKa = 9.95IRR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84MHH106 pKa = 5.87KK107 pKa = 9.82PFVHH111 pKa = 6.22RR112 pKa = 11.84LVRR115 pKa = 11.84HH116 pKa = 5.65CRR118 pKa = 11.84GEE120 pKa = 3.81LGQRR124 pKa = 11.84VHH126 pKa = 6.57TPPNVMVVEE135 pKa = 4.85RR136 pKa = 11.84TARR139 pKa = 11.84AYY141 pKa = 10.46CHH143 pKa = 5.13EE144 pKa = 4.56HH145 pKa = 5.52YY146 pKa = 10.75VRR148 pKa = 11.84SSDD151 pKa = 3.2ISCILPVVVALYY163 pKa = 9.29FFSRR167 pKa = 11.84SDD169 pKa = 3.31VQIRR173 pKa = 11.84TEE175 pKa = 3.8ALKK178 pKa = 10.7QSSAFVKK185 pKa = 10.02SSKK188 pKa = 7.81PHH190 pKa = 6.0RR191 pKa = 11.84FVGVGGRR198 pKa = 11.84QHH200 pKa = 6.37GVADD204 pKa = 3.77AA205 pKa = 4.29

Molecular weight: 23.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

923

205

491

307.7

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.717 ± 0.818

1.95 ± 0.357

5.85 ± 0.717

4.875 ± 0.915

4.875 ± 0.346

6.501 ± 0.332

2.925 ± 0.71

5.309 ± 1.02

5.959 ± 0.759

7.259 ± 0.443

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.142 ± 0.422

4.984 ± 1.237

4.55 ± 0.257

2.6 ± 0.215

6.717 ± 1.593

8.126 ± 0.628

4.984 ± 1.469

7.692 ± 0.762

1.517 ± 0.252

3.467 ± 0.778
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski