Salipiger aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Salipiger

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327YEX1|A0A327YEX1_9RHOB Heterotetrameric sarcosine oxidase gamma subunit OS=Salipiger aestuarii OX=568098 GN=ATI53_1010103 PE=4 SV=1
MM1 pKa = 6.96ITRR4 pKa = 11.84FAIALAILGALSACNGNTSSGDD26 pKa = 3.71DD27 pKa = 3.48EE28 pKa = 4.81STDD31 pKa = 3.19EE32 pKa = 4.45DD33 pKa = 4.42TVEE36 pKa = 4.52EE37 pKa = 4.57PDD39 pKa = 3.97SFPLPGSRR47 pKa = 11.84VNPTASQSITRR58 pKa = 11.84MEE60 pKa = 4.16VDD62 pKa = 3.84DD63 pKa = 4.39GAGNGMIVDD72 pKa = 4.24PTYY75 pKa = 11.42DD76 pKa = 3.89PDD78 pKa = 3.79TDD80 pKa = 3.47TFYY83 pKa = 11.67VDD85 pKa = 4.28NLAFDD90 pKa = 4.38GANTYY95 pKa = 10.66ARR97 pKa = 11.84ADD99 pKa = 4.05FSPLAEE105 pKa = 4.05ITGALGPFAVYY116 pKa = 10.47EE117 pKa = 4.15NADD120 pKa = 3.79TVTDD124 pKa = 4.11PVNGDD129 pKa = 3.91VIPQLQHH136 pKa = 5.55KK137 pKa = 9.63AIYY140 pKa = 9.89AVSQSGQTEE149 pKa = 3.99LAIVRR154 pKa = 11.84TGAFVDD160 pKa = 3.7YY161 pKa = 10.88GFGGFVYY168 pKa = 10.2QRR170 pKa = 11.84NGGVTIPTSGQASYY184 pKa = 10.62SGSYY188 pKa = 10.32GGLRR192 pKa = 11.84DD193 pKa = 3.64YY194 pKa = 11.26QGTGKK199 pKa = 9.53IEE201 pKa = 4.23YY202 pKa = 8.47STADD206 pKa = 3.26AQVYY210 pKa = 10.38IDD212 pKa = 4.31FEE214 pKa = 5.05DD215 pKa = 4.16YY216 pKa = 11.5NEE218 pKa = 4.3GDD220 pKa = 3.86GVRR223 pKa = 11.84GAITDD228 pKa = 3.5RR229 pKa = 11.84RR230 pKa = 11.84IYY232 pKa = 10.64DD233 pKa = 3.52SNGVDD238 pKa = 3.27ISTDD242 pKa = 3.39YY243 pKa = 11.79VNIPTIQFEE252 pKa = 4.21IGPGVARR259 pKa = 11.84SNGEE263 pKa = 3.61MSGTAFSMVDD273 pKa = 3.28GATHH277 pKa = 5.5EE278 pKa = 4.45TGSFYY283 pKa = 11.36AVLSDD288 pKa = 4.32GADD291 pKa = 3.28NDD293 pKa = 3.67VTAEE297 pKa = 4.25EE298 pKa = 4.12IVGIIVIEE306 pKa = 4.24GTVGDD311 pKa = 3.91TLARR315 pKa = 11.84EE316 pKa = 4.28TGGFMVTRR324 pKa = 4.74

Molecular weight: 34.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327XUZ6|A0A327XUZ6_9RHOB Tol-Pal system protein TolB OS=Salipiger aestuarii OX=568098 GN=tolB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.41ILNARR34 pKa = 11.84RR35 pKa = 11.84VRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.6QLSAA44 pKa = 3.97

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4452

0

4452

1363853

39

2139

306.3

33.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.701 ± 0.057

0.948 ± 0.013

6.356 ± 0.038

5.324 ± 0.035

3.567 ± 0.025

8.907 ± 0.037

2.092 ± 0.02

4.888 ± 0.026

2.734 ± 0.029

10.106 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.777 ± 0.019

2.383 ± 0.021

5.298 ± 0.03

3.123 ± 0.019

7.334 ± 0.041

5.126 ± 0.027

5.536 ± 0.027

7.233 ± 0.035

1.428 ± 0.016

2.14 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski