Ruminococcus sp. CAG:382

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6WD79|R6WD79_9FIRM RNA polymerase sigma factor OS=Ruminococcus sp. CAG:382 OX=1262957 GN=BN636_00834 PE=3 SV=1
MM1 pKa = 8.01DD2 pKa = 5.05GAHH5 pKa = 6.59DD6 pKa = 3.85TGLDD10 pKa = 3.24GSVIIKK16 pKa = 10.14SLSHH20 pKa = 6.52RR21 pKa = 11.84SEE23 pKa = 4.1AVGGAGSSGDD33 pKa = 3.54NLVVLGEE40 pKa = 4.14GLFVDD45 pKa = 4.72IEE47 pKa = 4.29NDD49 pKa = 3.42GLQVIACRR57 pKa = 11.84SGDD60 pKa = 3.76NNLLCACVDD69 pKa = 3.53MSHH72 pKa = 6.62GLFLGSIEE80 pKa = 4.19TGAFEE85 pKa = 4.35NDD87 pKa = 3.04VNIEE91 pKa = 4.08LTPGAVCCILFSIDD105 pKa = 4.21SDD107 pKa = 3.79FLTVNDD113 pKa = 4.74DD114 pKa = 4.39GIFGSFNGVLVFADD128 pKa = 3.67TTEE131 pKa = 3.55VRR133 pKa = 11.84TLSGIILEE141 pKa = 4.85KK142 pKa = 10.32MSEE145 pKa = 3.92HH146 pKa = 6.69CGAGEE151 pKa = 4.54VVDD154 pKa = 4.75SDD156 pKa = 4.75DD157 pKa = 5.16FIAFSAEE164 pKa = 3.6HH165 pKa = 6.07LAEE168 pKa = 4.85SEE170 pKa = 4.3TTDD173 pKa = 3.14AAEE176 pKa = 4.4TINCNFYY183 pKa = 10.84GHH185 pKa = 7.2FIFLQQ190 pKa = 3.55

Molecular weight: 19.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6W6M1|R6W6M1_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:382 OX=1262957 GN=BN636_01033 PE=4 SV=1
MM1 pKa = 7.82IKK3 pKa = 10.25RR4 pKa = 11.84KK5 pKa = 9.44LGHH8 pKa = 6.54IIKK11 pKa = 10.21SVEE14 pKa = 3.68HH15 pKa = 5.41TRR17 pKa = 11.84RR18 pKa = 11.84AVNAISFIDD27 pKa = 4.75FICAIVFALFYY38 pKa = 10.24KK39 pKa = 10.15SCQINVIIRR48 pKa = 11.84SIDD51 pKa = 3.21RR52 pKa = 11.84NIYY55 pKa = 6.28TRR57 pKa = 11.84RR58 pKa = 11.84IVIRR62 pKa = 11.84YY63 pKa = 6.19MLINIVNRR71 pKa = 11.84SFYY74 pKa = 10.77DD75 pKa = 3.02RR76 pKa = 11.84RR77 pKa = 11.84GNIGIGICRR86 pKa = 11.84FPSAIPFGKK95 pKa = 10.24SSGNIFFCRR104 pKa = 11.84FLIEE108 pKa = 4.31SDD110 pKa = 2.89VDD112 pKa = 3.86NIVVHH117 pKa = 6.4DD118 pKa = 3.85AVGRR122 pKa = 11.84FRR124 pKa = 11.84FII126 pKa = 3.93

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1853

0

1853

540255

31

1817

291.6

32.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.615 ± 0.06

1.861 ± 0.026

6.232 ± 0.045

6.564 ± 0.053

4.458 ± 0.045

7.323 ± 0.049

1.638 ± 0.027

6.892 ± 0.05

6.118 ± 0.044

8.598 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.024

4.212 ± 0.041

3.46 ± 0.032

2.059 ± 0.027

4.975 ± 0.052

6.606 ± 0.051

5.78 ± 0.046

7.138 ± 0.052

0.746 ± 0.017

4.093 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski