Pseudomonas sp. v388

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4804 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A427DQ00|A0A427DQ00_9PSED GNAT family N-acetyltransferase OS=Pseudomonas sp. v388 OX=2479849 GN=EGJ27_17620 PE=4 SV=1
MM1 pKa = 7.15QIKK4 pKa = 7.98TALLLANPCDD14 pKa = 4.88DD15 pKa = 5.0EE16 pKa = 6.28NDD18 pKa = 3.83DD19 pKa = 4.28MALLCCHH26 pKa = 7.14DD27 pKa = 4.33EE28 pKa = 3.99NHH30 pKa = 6.47EE31 pKa = 4.24LFSLARR37 pKa = 11.84FPDD40 pKa = 3.6EE41 pKa = 5.04DD42 pKa = 3.65EE43 pKa = 4.86VEE45 pKa = 4.06ITVCDD50 pKa = 3.44DD51 pKa = 3.36TVYY54 pKa = 9.41VTSLKK59 pKa = 9.32VTLSATQLVVEE70 pKa = 5.34ISAEE74 pKa = 3.95DD75 pKa = 3.73AEE77 pKa = 4.67ALGGDD82 pKa = 3.35TRR84 pKa = 11.84YY85 pKa = 10.57EE86 pKa = 4.16IVHH89 pKa = 5.61GTDD92 pKa = 2.94PGEE95 pKa = 3.81WAEE98 pKa = 4.02LQEE101 pKa = 4.31TLRR104 pKa = 11.84IILDD108 pKa = 3.47NVGTYY113 pKa = 9.54VCEE116 pKa = 4.21INN118 pKa = 4.25

Molecular weight: 13.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A427DZA4|A0A427DZA4_9PSED Alanine racemase OS=Pseudomonas sp. v388 OX=2479849 GN=alr PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4804

0

4804

1594406

24

4381

331.9

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.882 ± 0.043

0.951 ± 0.013

5.499 ± 0.026

5.66 ± 0.03

3.627 ± 0.022

7.896 ± 0.032

2.249 ± 0.017

4.894 ± 0.03

3.437 ± 0.028

11.575 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.019

3.016 ± 0.02

4.902 ± 0.025

4.496 ± 0.026

6.523 ± 0.034

5.893 ± 0.027

5.024 ± 0.032

7.199 ± 0.032

1.414 ± 0.017

2.492 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski