Sparassis crispa

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Sparassidaceae; Sparassis

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401GL46|A0A401GL46_9APHY Non-specific serine/threonine protein kinase OS=Sparassis crispa OX=139825 GN=SCP_0412730 PE=4 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84SKK5 pKa = 10.7LQSAVGGGAKK15 pKa = 10.35DD16 pKa = 3.25STSLHH21 pKa = 5.62RR22 pKa = 11.84WVLLKK27 pKa = 10.99NSIVRR32 pKa = 11.84SSPSDD37 pKa = 3.08ATTATPEE44 pKa = 4.33GADD47 pKa = 3.4VTDD50 pKa = 4.62VYY52 pKa = 11.15PHH54 pKa = 7.4DD55 pKa = 4.71DD56 pKa = 3.99EE57 pKa = 5.57EE58 pKa = 4.52VHH60 pKa = 6.8EE61 pKa = 5.83DD62 pKa = 3.61EE63 pKa = 6.62DD64 pKa = 4.22SDD66 pKa = 5.41AFMFPDD72 pKa = 4.11PDD74 pKa = 3.46AVRR77 pKa = 11.84GSRR80 pKa = 11.84DD81 pKa = 3.09SGLCDD86 pKa = 5.46PEE88 pKa = 4.36NQWLDD93 pKa = 3.55SLLQTLGDD101 pKa = 4.1DD102 pKa = 4.12DD103 pKa = 3.94QAEE106 pKa = 4.41SEE108 pKa = 4.79VPVNAADD115 pKa = 5.7DD116 pKa = 4.4DD117 pKa = 4.59DD118 pKa = 5.5EE119 pKa = 4.92PLSPLCSPMSSSDD132 pKa = 3.39DD133 pKa = 3.72LVNHH137 pKa = 6.33SSYY140 pKa = 11.77YY141 pKa = 10.25EE142 pKa = 3.84IQPPLSIPYY151 pKa = 8.16PVPYY155 pKa = 9.92PPLHH159 pKa = 6.73PPLVPAWFEE168 pKa = 4.28DD169 pKa = 4.06EE170 pKa = 4.66SPSDD174 pKa = 3.77SLLDD178 pKa = 3.69SSPPLYY184 pKa = 10.35HH185 pKa = 7.72DD186 pKa = 4.25PLPYY190 pKa = 10.42YY191 pKa = 10.67DD192 pKa = 4.92VDD194 pKa = 3.82DD195 pKa = 4.72TEE197 pKa = 5.67DD198 pKa = 3.81LAVPDD203 pKa = 5.32AIEE206 pKa = 4.57DD207 pKa = 3.95TSDD210 pKa = 4.1DD211 pKa = 3.89EE212 pKa = 5.66SDD214 pKa = 4.03APSTPCYY221 pKa = 10.23RR222 pKa = 11.84STTSLSPTDD231 pKa = 3.79PASIPLPPEE240 pKa = 3.53QTRR243 pKa = 11.84LRR245 pKa = 11.84SHH247 pKa = 7.4PLQPRR252 pKa = 11.84VYY254 pKa = 9.95IDD256 pKa = 3.33TDD258 pKa = 3.16DD259 pKa = 3.97SYY261 pKa = 11.71FYY263 pKa = 10.65PFDD266 pKa = 3.82LTSMPSHH273 pKa = 7.6DD274 pKa = 4.96DD275 pKa = 3.27DD276 pKa = 5.58HH277 pKa = 7.12PISARR282 pKa = 11.84VFQPPIYY289 pKa = 9.9QEE291 pKa = 4.2CC292 pKa = 3.48

Molecular weight: 32.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A401GVM3|A0A401GVM3_9APHY Glycerol kinase OS=Sparassis crispa OX=139825 GN=SCP_0901190 PE=3 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84KK3 pKa = 9.0PLQQRR8 pKa = 11.84VGTLGRR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 9.04SAFRR22 pKa = 11.84LPSNSAVPIPSNPAISAQARR42 pKa = 11.84VLASLTRR49 pKa = 11.84LASFSAPAFIAAGALSAGTQSAPRR73 pKa = 11.84GFPDD77 pKa = 3.68PVKK80 pKa = 10.14PHH82 pKa = 6.06HH83 pKa = 6.31TPPRR87 pKa = 11.84LPAPFLDD94 pKa = 4.58LPRR97 pKa = 11.84HH98 pKa = 4.66VQAHH102 pKa = 5.88RR103 pKa = 11.84SQRR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84SRR112 pKa = 11.84TGHH115 pKa = 6.74LGATTACSSLLAVTQAPTKK134 pKa = 10.44IPTLPSTLVAFAHH147 pKa = 6.41CGEE150 pKa = 4.54RR151 pKa = 11.84PHH153 pKa = 6.73PGRR156 pKa = 11.84RR157 pKa = 11.84AGHH160 pKa = 6.57RR161 pKa = 11.84GATTAPSPSSRR172 pKa = 11.84PPSPRR177 pKa = 11.84RR178 pKa = 11.84GPSSPLRR185 pKa = 11.84TLALPSSTRR194 pKa = 11.84VRR196 pKa = 11.84PLRR199 pKa = 3.94

Molecular weight: 21.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13078

0

13078

5783113

49

5044

442.2

48.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.85 ± 0.019

1.301 ± 0.01

5.751 ± 0.016

6.085 ± 0.022

3.565 ± 0.014

6.261 ± 0.018

2.71 ± 0.011

4.553 ± 0.015

4.364 ± 0.02

9.17 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.008

3.188 ± 0.011

6.707 ± 0.025

3.704 ± 0.013

6.485 ± 0.016

8.604 ± 0.033

5.871 ± 0.013

6.564 ± 0.016

1.421 ± 0.008

2.631 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski