Bacillus phage MG-B1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Northropvirinae; Klosterneuburgvirus; Bacillus virus MGB1

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4WNI2|M4WNI2_9CAUD Uncharacterized protein OS=Bacillus phage MG-B1 OX=1309583 GN=mgb1_012 PE=4 SV=1
MM1 pKa = 7.35IQTKK5 pKa = 8.43TLDD8 pKa = 3.58FTGKK12 pKa = 9.62ISGSITQNPNIAKK25 pKa = 8.98MNGVEE30 pKa = 4.54FTQTQYY36 pKa = 12.11NLIFGSEE43 pKa = 4.24LSVASTDD50 pKa = 3.2EE51 pKa = 4.1LTFEE55 pKa = 4.44FPVPNDD61 pKa = 3.31VKK63 pKa = 11.21VGLQFVQIYY72 pKa = 8.71FKK74 pKa = 11.0SKK76 pKa = 8.65VVSGEE81 pKa = 3.99VEE83 pKa = 3.98LRR85 pKa = 11.84SSDD88 pKa = 3.18KK89 pKa = 11.07GVYY92 pKa = 10.23NDD94 pKa = 3.79DD95 pKa = 3.48TVIYY99 pKa = 9.02KK100 pKa = 10.18QKK102 pKa = 10.16FVQISPSEE110 pKa = 3.99IEE112 pKa = 3.69NDD114 pKa = 3.59VIKK117 pKa = 10.85FNIKK121 pKa = 8.91STDD124 pKa = 3.27DD125 pKa = 3.43YY126 pKa = 11.74GVYY129 pKa = 9.43EE130 pKa = 4.35VKK132 pKa = 10.51CDD134 pKa = 5.17CIYY137 pKa = 11.44DD138 pKa = 4.11DD139 pKa = 6.09DD140 pKa = 4.77GLNSYY145 pKa = 9.73SGNYY149 pKa = 8.55TYY151 pKa = 11.18LNSSSISTTVTITSKK166 pKa = 10.93KK167 pKa = 9.87LADD170 pKa = 3.36IMTEE174 pKa = 4.15LEE176 pKa = 4.24SLPVDD181 pKa = 3.3SLTYY185 pKa = 8.22TTFAQVPSHH194 pKa = 7.52DD195 pKa = 4.14DD196 pKa = 3.21VV197 pKa = 5.13

Molecular weight: 21.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4W8C3|M4W8C3_9CAUD Uncharacterized protein OS=Bacillus phage MG-B1 OX=1309583 GN=mgb1_018 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.33KK3 pKa = 8.89RR4 pKa = 11.84TEE6 pKa = 4.01DD7 pKa = 3.77LIKK10 pKa = 10.53RR11 pKa = 11.84AMKK14 pKa = 8.16EE15 pKa = 3.48TGYY18 pKa = 8.99TRR20 pKa = 11.84EE21 pKa = 3.92EE22 pKa = 4.31VIKK25 pKa = 10.74HH26 pKa = 5.51ILTFHH31 pKa = 6.31VPLKK35 pKa = 10.67KK36 pKa = 9.83MKK38 pKa = 10.51NPLRR42 pKa = 11.84KK43 pKa = 9.47FKK45 pKa = 11.11RR46 pKa = 11.84NDD48 pKa = 2.91VRR50 pKa = 4.76

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

7699

30

703

183.3

21.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.858 ± 0.312

1.234 ± 0.17

6.092 ± 0.333

7.611 ± 0.574

4.481 ± 0.376

6.429 ± 0.468

1.74 ± 0.124

7.144 ± 0.372

9.209 ± 0.622

7.585 ± 0.429

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.286 ± 0.238

7.001 ± 0.404

2.715 ± 0.179

3.442 ± 0.236

3.975 ± 0.294

5.845 ± 0.339

6.183 ± 0.446

6.338 ± 0.351

1.221 ± 0.159

4.611 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski