Rhizobium subbaraonis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285UYQ2|A0A285UYQ2_9RHIZ Type IV secretion system protein VirB3 OS=Rhizobium subbaraonis OX=908946 GN=SAMN05892877_12543 PE=4 SV=1
MM1 pKa = 7.06GVRR4 pKa = 11.84YY5 pKa = 9.88VKK7 pKa = 10.71YY8 pKa = 8.84RR9 pKa = 11.84TDD11 pKa = 3.12TGEE14 pKa = 4.07IIATGTCSNQEE25 pKa = 3.74HH26 pKa = 7.1CFLQMEE32 pKa = 4.5DD33 pKa = 4.32AEE35 pKa = 4.56NEE37 pKa = 4.29HH38 pKa = 6.8ILLDD42 pKa = 3.89VSADD46 pKa = 5.0DD47 pKa = 3.55ATQWIDD53 pKa = 3.92DD54 pKa = 3.81QGEE57 pKa = 4.01LADD60 pKa = 5.57RR61 pKa = 11.84PVLDD65 pKa = 5.02LPTATSIAADD75 pKa = 4.98GIAEE79 pKa = 4.32VVGALPAGTVVRR91 pKa = 11.84FNGAAATTDD100 pKa = 3.22GAAFAFTTDD109 pKa = 3.04MPGTYY114 pKa = 10.12VFDD117 pKa = 4.48FEE119 pKa = 5.38PPFPYY124 pKa = 9.92IPCTLTVEE132 pKa = 4.25ATDD135 pKa = 4.98AII137 pKa = 4.34

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285V268|A0A285V268_9RHIZ Histidine ammonia-lyase OS=Rhizobium subbaraonis OX=908946 GN=SAMN05892877_14414 PE=4 SV=1
MM1 pKa = 7.63SPEE4 pKa = 3.96RR5 pKa = 11.84SKK7 pKa = 11.39LPWIWVVKK15 pKa = 8.73PPRR18 pKa = 11.84VVPPPGLFAPFCSGRR33 pKa = 11.84RR34 pKa = 11.84DD35 pKa = 4.28SRR37 pKa = 11.84EE38 pKa = 3.36QWSNRR43 pKa = 11.84TSEE46 pKa = 4.04SGAPMGAKK54 pKa = 9.48VSKK57 pKa = 10.04KK58 pKa = 9.74ASTRR62 pKa = 11.84RR63 pKa = 11.84PCSADD68 pKa = 3.14RR69 pKa = 11.84SAPRR73 pKa = 11.84PSSICSDD80 pKa = 3.14RR81 pKa = 11.84RR82 pKa = 11.84DD83 pKa = 3.47AGGLGISIRR92 pKa = 11.84WACKK96 pKa = 8.06VLRR99 pKa = 11.84FDD101 pKa = 3.68TSTYY105 pKa = 9.27HH106 pKa = 5.16YY107 pKa = 10.04KK108 pKa = 10.26SRR110 pKa = 11.84RR111 pKa = 11.84TGQAPLEE118 pKa = 4.09RR119 pKa = 11.84RR120 pKa = 11.84IKK122 pKa = 10.13EE123 pKa = 4.27YY124 pKa = 10.76VRR126 pKa = 11.84HH127 pKa = 5.59ACGMATAVRR136 pKa = 11.84MVTCAAMDD144 pKa = 3.66GRR146 pKa = 11.84STINMKK152 pKa = 8.54MRR154 pKa = 11.84RR155 pKa = 11.84FRR157 pKa = 11.84LFIDD161 pKa = 3.89RR162 pKa = 11.84CCLYY166 pKa = 10.95VADD169 pKa = 4.85SPKK172 pKa = 10.55KK173 pKa = 10.47LQILLQINSSEE184 pKa = 4.09CRR186 pKa = 11.84SADD189 pKa = 3.26VAFVHH194 pKa = 6.99FKK196 pKa = 10.87RR197 pKa = 11.84SIHH200 pKa = 5.7TAMQTLSGEE209 pKa = 4.28SSGLYY214 pKa = 9.58QCFLRR219 pKa = 11.84ADD221 pKa = 3.46TDD223 pKa = 4.11YY224 pKa = 11.26ILPRR228 pKa = 11.84DD229 pKa = 3.42IYY231 pKa = 10.91QRR233 pKa = 11.84TRR235 pKa = 11.84SPLL238 pKa = 3.33

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6271

0

6271

1918300

27

3078

305.9

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.268 ± 0.039

0.814 ± 0.01

5.743 ± 0.026

5.867 ± 0.03

3.83 ± 0.023

8.497 ± 0.03

2.007 ± 0.016

5.416 ± 0.024

3.447 ± 0.025

9.881 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.014

2.7 ± 0.016

4.905 ± 0.025

3.071 ± 0.017

7.101 ± 0.037

5.637 ± 0.021

5.32 ± 0.022

7.389 ± 0.027

1.302 ± 0.012

2.272 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski