Ralstonia phage DU_RP_II

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221SAP9|A0A221SAP9_9CAUD Uncharacterized protein OS=Ralstonia phage DU_RP_II OX=2022331 GN=R2B_p031 PE=4 SV=1
MM1 pKa = 7.63AIEE4 pKa = 4.37IRR6 pKa = 11.84NRR8 pKa = 11.84WTGAVILTVEE18 pKa = 4.27AEE20 pKa = 4.19NLRR23 pKa = 11.84DD24 pKa = 3.67ADD26 pKa = 5.11LRR28 pKa = 11.84DD29 pKa = 3.65ADD31 pKa = 5.03LSYY34 pKa = 11.91ADD36 pKa = 4.75LRR38 pKa = 11.84GANLSYY44 pKa = 11.57ADD46 pKa = 4.87LSDD49 pKa = 5.16ANLSYY54 pKa = 11.7ADD56 pKa = 4.92LSCADD61 pKa = 5.57LIYY64 pKa = 11.07ADD66 pKa = 5.38LSDD69 pKa = 5.21ANLIYY74 pKa = 11.05ANLIDD79 pKa = 5.63ANLSCANLIDD89 pKa = 4.39ANLRR93 pKa = 11.84GANLSYY99 pKa = 11.29ANPRR103 pKa = 11.84DD104 pKa = 3.99ANLSGANLSDD114 pKa = 4.19ANLSYY119 pKa = 11.71ADD121 pKa = 4.76LSDD124 pKa = 4.67ANLRR128 pKa = 11.84DD129 pKa = 4.11ANLSDD134 pKa = 3.9ANLRR138 pKa = 11.84GANLSDD144 pKa = 4.23ANLSYY149 pKa = 11.71ADD151 pKa = 4.76LSDD154 pKa = 4.67ANLRR158 pKa = 11.84DD159 pKa = 3.82ANLSYY164 pKa = 11.12AAGNLKK170 pKa = 9.1QVKK173 pKa = 9.64SIHH176 pKa = 6.19ADD178 pKa = 2.92RR179 pKa = 11.84WVVTYY184 pKa = 8.92TADD187 pKa = 3.05TMFIGCQTHH196 pKa = 7.37ALTDD200 pKa = 2.84WWGFDD205 pKa = 3.09NRR207 pKa = 11.84TINAMDD213 pKa = 3.8TGALKK218 pKa = 9.52WWRR221 pKa = 11.84KK222 pKa = 6.2WKK224 pKa = 9.79PVLQAIIEE232 pKa = 4.23ASPAEE237 pKa = 4.03PTKK240 pKa = 10.79PEE242 pKa = 4.02TEE244 pKa = 3.95AAPP247 pKa = 3.71

Molecular weight: 26.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221SAL7|A0A221SAL7_9CAUD Uncharacterized protein OS=Ralstonia phage DU_RP_II OX=2022331 GN=R2B_p010 PE=4 SV=1
MM1 pKa = 7.46SLLEE5 pKa = 3.97RR6 pKa = 11.84AKK8 pKa = 10.22QYY10 pKa = 10.95LFRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84TAYY18 pKa = 10.47VKK20 pKa = 10.07TFLSPYY26 pKa = 8.82GDD28 pKa = 3.2EE29 pKa = 4.31VLRR32 pKa = 11.84DD33 pKa = 3.3LARR36 pKa = 11.84FCRR39 pKa = 11.84ANVSTFHH46 pKa = 6.44TDD48 pKa = 2.82ARR50 pKa = 11.84AHH52 pKa = 5.8AVAEE56 pKa = 4.37GRR58 pKa = 11.84RR59 pKa = 11.84EE60 pKa = 3.6VWLRR64 pKa = 11.84IQMHH68 pKa = 6.61LRR70 pKa = 11.84LTDD73 pKa = 3.64DD74 pKa = 4.35EE75 pKa = 4.47LWALIGQSSQPIRR88 pKa = 11.84KK89 pKa = 8.98PEE91 pKa = 3.74EE92 pKa = 3.66

Molecular weight: 10.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

12046

58

1069

317.0

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.058 ± 0.568

0.88 ± 0.191

5.786 ± 0.282

5.238 ± 0.57

3.063 ± 0.196

8.517 ± 0.458

1.669 ± 0.186

4.259 ± 0.282

4.209 ± 0.359

7.787 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.549 ± 0.2

4.109 ± 0.369

4.707 ± 0.256

4.898 ± 0.416

6.268 ± 0.45

5.778 ± 0.433

6.334 ± 0.588

6.342 ± 0.36

1.702 ± 0.162

2.847 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski