Luteimonas cucumeris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Luteimonas

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A562KUE3|A0A562KUE3_9GAMM Uncharacterized protein DUF4177 OS=Luteimonas cucumeris OX=985012 GN=IP90_03228 PE=4 SV=1
MM1 pKa = 7.35NKK3 pKa = 9.03VFSLVWSRR11 pKa = 11.84SLQALVVASEE21 pKa = 4.13LASANGRR28 pKa = 11.84KK29 pKa = 9.61AGRR32 pKa = 11.84PRR34 pKa = 11.84RR35 pKa = 11.84LAHH38 pKa = 6.19AAFGVVVLSASFSLAPLAQAAEE60 pKa = 4.47SGKK63 pKa = 8.05PTCTVNLPGQAGVAVDD79 pKa = 4.46CAQLQQIVATAQRR92 pKa = 11.84AGDD95 pKa = 3.66PRR97 pKa = 11.84KK98 pKa = 9.98GRR100 pKa = 11.84KK101 pKa = 8.66ARR103 pKa = 11.84PVVDD107 pKa = 5.12SFDD110 pKa = 3.63SDD112 pKa = 3.84YY113 pKa = 11.44FKK115 pKa = 11.52ANGTGDD121 pKa = 3.88GDD123 pKa = 3.83DD124 pKa = 4.95ASAQGEE130 pKa = 4.21GAIAIGSGAQTRR142 pKa = 11.84PWVSDD147 pKa = 3.22EE148 pKa = 4.27LGEE151 pKa = 4.35SFEE154 pKa = 4.92AEE156 pKa = 3.79GAVAIGRR163 pKa = 11.84QSVAEE168 pKa = 4.25GWEE171 pKa = 4.11ATALGANSFASSNSATAVGGEE192 pKa = 4.19SGAFGIGSSAFGQGSKK208 pKa = 11.0AFGHH212 pKa = 6.93AATALGQGAMTFGFGNTAVGASAMASAEE240 pKa = 4.02QAATALGALSEE251 pKa = 4.16AWSNGSTAVGGMAIAAGEE269 pKa = 4.22GSLAAGFDD277 pKa = 3.79SQAGGFEE284 pKa = 4.42SVALGGHH291 pKa = 4.75TRR293 pKa = 11.84VGNGEE298 pKa = 4.05AGVGVGANAVALGNGAVGVGNNSLANGSNTVAMGRR333 pKa = 11.84NAAANSEE340 pKa = 4.1GALAIGGSEE349 pKa = 4.03WTDD352 pKa = 3.02HH353 pKa = 7.32DD354 pKa = 5.0GDD356 pKa = 3.89GMPDD360 pKa = 3.08VGEE363 pKa = 4.07ATLAEE368 pKa = 4.42GQNAVAIGGLAGASGDD384 pKa = 3.45NAQAIGHH391 pKa = 5.58QANALAEE398 pKa = 4.2NATATGAMSMAAGEE412 pKa = 4.24NSTATGASSVANGIDD427 pKa = 3.2SSAYY431 pKa = 9.48GAQSQADD438 pKa = 4.12GDD440 pKa = 3.94HH441 pKa = 5.95STAIGSGSLALGVGSSAVGSGANAFGEE468 pKa = 4.29YY469 pKa = 10.14SVANGYY475 pKa = 10.26GSAAEE480 pKa = 4.33GADD483 pKa = 3.15AVAVGSNSAASGSGASALGAAAQAVGTQSAAMGFNSQALEE523 pKa = 4.03MATTAVGGQTVANQWFATAIGYY545 pKa = 8.85GSQATGYY552 pKa = 10.27SSVALGEE559 pKa = 4.31NVLATGEE566 pKa = 3.93MSIAIGNSWAGMMQTQSNGNMSVAIGSAAATWDD599 pKa = 3.57NYY601 pKa = 9.51GVAVGSLAGANTEE614 pKa = 4.01YY615 pKa = 9.6STSVGAMSAAIGQQSTALGAAAMAEE640 pKa = 4.19ADD642 pKa = 3.75DD643 pKa = 4.4SVAIGYY649 pKa = 8.99YY650 pKa = 9.87SHH652 pKa = 7.34ASGANSVALGNEE664 pKa = 4.43SVASRR669 pKa = 11.84DD670 pKa = 3.43NTISVGKK677 pKa = 10.17AGSEE681 pKa = 3.76RR682 pKa = 11.84QITNVAAGTEE692 pKa = 4.09ATDD695 pKa = 4.07AVNKK699 pKa = 10.14AQLDD703 pKa = 4.02EE704 pKa = 4.44VATGVADD711 pKa = 3.93ANHH714 pKa = 5.87YY715 pKa = 9.26FAANGAADD723 pKa = 4.09GSDD726 pKa = 3.4DD727 pKa = 3.8AMASGDD733 pKa = 3.74HH734 pKa = 6.32AVATGAYY741 pKa = 8.86AVANGIEE748 pKa = 4.2SSAYY752 pKa = 9.91GYY754 pKa = 10.39QSQADD759 pKa = 3.72GDD761 pKa = 4.06YY762 pKa = 10.72SQAIGSGSLAMGVGTSAVGSGANAIGNGASAFGAGAVAFLDD803 pKa = 4.06GATAVGSNAQTAGLNTVAVGNGAAASATGAVAIGGQATNGLGQPILADD851 pKa = 3.79GGTSEE856 pKa = 5.03PLGAAQAAEE865 pKa = 4.25LSTAVGASTYY875 pKa = 10.17ATGNWSAAYY884 pKa = 9.7GVGAWSAGSQSTAVGALAIAYY905 pKa = 8.21GDD907 pKa = 3.68SSSAFGTNTMASGQSSTAIGQSAWALGDD935 pKa = 3.42YY936 pKa = 10.57SVAIGVAANTSGSNTVAIGNSATVFDD962 pKa = 4.38YY963 pKa = 9.75STWSNVSDD971 pKa = 3.65AVALGSASMASADD984 pKa = 3.53HH985 pKa = 6.65AVALGSNSHH994 pKa = 6.16AMADD998 pKa = 3.44NSVALGQGSLADD1010 pKa = 3.9RR1011 pKa = 11.84ANTVSVGAAEE1021 pKa = 4.17AWTDD1025 pKa = 3.7LLGTSHH1031 pKa = 6.68EE1032 pKa = 4.76AIDD1035 pKa = 3.76RR1036 pKa = 11.84QITNVAAGTEE1046 pKa = 4.09ATDD1049 pKa = 4.99AVNKK1053 pKa = 10.24DD1054 pKa = 3.52QLDD1057 pKa = 3.67DD1058 pKa = 3.38ATRR1061 pKa = 11.84YY1062 pKa = 10.34LEE1064 pKa = 4.59ANGAGDD1070 pKa = 3.92GSDD1073 pKa = 3.77DD1074 pKa = 4.17AVATGQYY1081 pKa = 8.6ATATGANSIANGAEE1095 pKa = 3.83SSVYY1099 pKa = 10.3GYY1101 pKa = 10.58QSQADD1106 pKa = 3.72GDD1108 pKa = 4.17YY1109 pKa = 9.56ATAIGSSSYY1118 pKa = 11.31AGALGANAIGTGATALGEE1136 pKa = 4.11TSNAIGYY1143 pKa = 9.85GSVATGLDD1151 pKa = 3.29AVAVGTSSNASGVNALAMGAGSSASADD1178 pKa = 3.11VSVAINTGSVAGLWGVAPGVNAAGGVGIGAAAVVGANADD1217 pKa = 3.46AGVALGLSATVVEE1230 pKa = 4.89GAYY1233 pKa = 10.04QGVAIGMGSYY1243 pKa = 10.46VGDD1246 pKa = 3.68AQGIAIGGFGARR1258 pKa = 11.84FIDD1261 pKa = 4.42GSIEE1265 pKa = 3.91LRR1267 pKa = 11.84PTVAMGTGAVAIGLGSEE1284 pKa = 4.42TFNDD1288 pKa = 3.36HH1289 pKa = 5.65ATALGAFSTVTAQNAVAIGANSLADD1314 pKa = 4.03RR1315 pKa = 11.84DD1316 pKa = 3.96NTLSVGYY1323 pKa = 9.92ADD1325 pKa = 4.8HH1326 pKa = 6.95EE1327 pKa = 4.25RR1328 pKa = 11.84QITHH1332 pKa = 5.84VAAGTEE1338 pKa = 4.03ATDD1341 pKa = 4.99AVNKK1345 pKa = 10.24DD1346 pKa = 3.52QLDD1349 pKa = 3.68DD1350 pKa = 3.5ATRR1353 pKa = 11.84YY1354 pKa = 8.88LASNGAGDD1362 pKa = 4.17GSDD1365 pKa = 3.84DD1366 pKa = 4.07AVATGDD1372 pKa = 3.48QAVATGASAVANGVGSSAYY1391 pKa = 9.97GSQGYY1396 pKa = 10.99ADD1398 pKa = 4.52GDD1400 pKa = 3.85YY1401 pKa = 10.04STAVGSGSLALGAGASAFGSGANALGEE1428 pKa = 4.12NSTALGTGALASGFGAYY1445 pKa = 10.5AMGNASNAAARR1456 pKa = 11.84NSLALGGMASVDD1468 pKa = 3.4QSSALGTAIGYY1479 pKa = 7.92GAYY1482 pKa = 9.54IGANSSAAIAMGFAAGVGDD1501 pKa = 3.88GASNSISMGNLSYY1514 pKa = 11.33ADD1516 pKa = 3.8SPYY1519 pKa = 10.31TIAIGSMFAYY1529 pKa = 10.23VNHH1532 pKa = 7.39DD1533 pKa = 3.55GGLGLATTGASGWGAVAFGPGAQALGDD1560 pKa = 3.74YY1561 pKa = 9.55TLALGPAASVTAANAVALGGLSIADD1586 pKa = 4.05RR1587 pKa = 11.84EE1588 pKa = 4.45NTVSVGAADD1597 pKa = 4.1HH1598 pKa = 6.29EE1599 pKa = 4.32RR1600 pKa = 11.84QIVNVAAGTEE1610 pKa = 4.0ATDD1613 pKa = 3.73AVNVAQLDD1621 pKa = 3.73DD1622 pKa = 3.47ATRR1625 pKa = 11.84YY1626 pKa = 9.3VAVNGAGDD1634 pKa = 3.82GSDD1637 pKa = 3.74DD1638 pKa = 4.31AVATGEE1644 pKa = 4.09NATATGASSVANGVEE1659 pKa = 4.09SSAYY1663 pKa = 10.03GSQSYY1668 pKa = 11.5ADD1670 pKa = 4.09ADD1672 pKa = 3.75YY1673 pKa = 10.0STALGSNSLALGIGASAIGSGANALGTNSVANGYY1707 pKa = 10.91ASVASGEE1714 pKa = 4.03LSIAQGSEE1722 pKa = 3.84SQALGEE1728 pKa = 4.03ASIALGTQSLAEE1740 pKa = 5.08GIDD1743 pKa = 3.92SIGVGAGSYY1752 pKa = 10.67ASVYY1756 pKa = 8.0GTAVGTLSQAGMYY1769 pKa = 10.44GSTALGHH1776 pKa = 6.2SATANNDD1783 pKa = 2.86FALALGAVSTSLAVGGIAIGTEE1805 pKa = 4.22SYY1807 pKa = 11.57VDD1809 pKa = 3.32ANAYY1813 pKa = 9.37QGIAIGSVVSAIGHH1827 pKa = 5.92RR1828 pKa = 11.84SIAIGSQWTTAGGDD1842 pKa = 3.52SAVAIGGTMACSGICIEE1859 pKa = 4.74QNNAAWGANSVALGAGTQAQADD1881 pKa = 4.18GATALGAGANAGGIGSVAAGQFAQAWSDD1909 pKa = 3.65DD1910 pKa = 3.47AVALGNTSQAIAAGSVALGAYY1931 pKa = 9.42SVADD1935 pKa = 3.81RR1936 pKa = 11.84DD1937 pKa = 3.99NTVSVGDD1944 pKa = 4.08AGTGLTRR1951 pKa = 11.84QITNVAAGTEE1961 pKa = 4.18ANDD1964 pKa = 4.01AVNKK1968 pKa = 9.86GQLDD1972 pKa = 3.96EE1973 pKa = 5.34LSDD1976 pKa = 4.3SIGDD1980 pKa = 3.62ANYY1983 pKa = 10.73YY1984 pKa = 9.66FAADD1988 pKa = 3.85GAGDD1992 pKa = 3.88GSDD1995 pKa = 3.25AAVASGVGSVAAGANSVASGTGTVAYY2021 pKa = 10.16GRR2023 pKa = 11.84GALASGEE2030 pKa = 3.96QAMAIGDD2037 pKa = 4.11GAQADD2042 pKa = 4.32YY2043 pKa = 9.88FYY2045 pKa = 11.75AMAIGAGAVSSSWASMAIGRR2065 pKa = 11.84NAIASGPRR2073 pKa = 11.84AVAIGEE2079 pKa = 4.1NAQAMDD2085 pKa = 4.11NGNVALGWASQSVGMFSAALGGSSAAIGWNTLAAGFGAWVLGNNATAVGAGAGSFYY2141 pKa = 10.66TVDD2144 pKa = 3.97EE2145 pKa = 4.24ITTVGSNARR2154 pKa = 11.84ADD2156 pKa = 3.79SFRR2159 pKa = 11.84GTVVGAGSVAAEE2171 pKa = 4.09WAPEE2175 pKa = 4.0STIVGANAFSFDD2187 pKa = 4.17SNGTAIGEE2195 pKa = 4.32SSQVSYY2201 pKa = 11.02GAANAVALGSGSHH2214 pKa = 7.05ADD2216 pKa = 3.66RR2217 pKa = 11.84ANTVSVGASGPWTDD2231 pKa = 3.43GFGVEE2236 pKa = 4.82HH2237 pKa = 7.46PALQRR2242 pKa = 11.84QITNVAAGTEE2252 pKa = 3.99ASDD2255 pKa = 4.44AVNKK2259 pKa = 10.29AQLDD2263 pKa = 3.88EE2264 pKa = 4.15VAAAGEE2270 pKa = 4.13ATGKK2274 pKa = 10.35FFF2276 pKa = 5.64

Molecular weight:
216.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A562LFD5|A0A562LFD5_9GAMM TetR family transcriptional regulator OS=Luteimonas cucumeris OX=985012 GN=IP90_00586 PE=4 SV=1
MM1 pKa = 7.41AVKK4 pKa = 10.23KK5 pKa = 10.04AAKK8 pKa = 9.47KK9 pKa = 10.42RR10 pKa = 11.84PAKK13 pKa = 10.19KK14 pKa = 9.78AAKK17 pKa = 8.48KK18 pKa = 7.32TAKK21 pKa = 9.03KK22 pKa = 6.66TTAKK26 pKa = 10.32KK27 pKa = 9.12ATRR30 pKa = 11.84KK31 pKa = 8.48VAKK34 pKa = 9.92KK35 pKa = 10.08AARR38 pKa = 11.84KK39 pKa = 7.18PAKK42 pKa = 9.89KK43 pKa = 9.97AVRR46 pKa = 11.84KK47 pKa = 8.88AAKK50 pKa = 10.02KK51 pKa = 9.89KK52 pKa = 8.43VAKK55 pKa = 9.97KK56 pKa = 10.19AVRR59 pKa = 11.84KK60 pKa = 8.84AVKK63 pKa = 10.2RR64 pKa = 11.84KK65 pKa = 8.03VAKK68 pKa = 10.1KK69 pKa = 9.16AAKK72 pKa = 9.71KK73 pKa = 10.15AVRR76 pKa = 11.84KK77 pKa = 8.9AAKK80 pKa = 10.02KK81 pKa = 9.89KK82 pKa = 8.43VAKK85 pKa = 10.07KK86 pKa = 10.23AVRR89 pKa = 11.84KK90 pKa = 8.72VAKK93 pKa = 10.31KK94 pKa = 10.02KK95 pKa = 8.89VAKK98 pKa = 10.27KK99 pKa = 10.06AVKK102 pKa = 9.9KK103 pKa = 10.01AAKK106 pKa = 9.87KK107 pKa = 8.93KK108 pKa = 7.13TAKK111 pKa = 8.99KK112 pKa = 7.45TAKK115 pKa = 10.09KK116 pKa = 9.81AAKK119 pKa = 9.34KK120 pKa = 10.09AKK122 pKa = 9.71KK123 pKa = 10.02KK124 pKa = 8.23PARR127 pKa = 11.84RR128 pKa = 11.84AKK130 pKa = 9.82KK131 pKa = 9.68AAPATMPAMPAPMII145 pKa = 4.36

Molecular weight:
15.58 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3213

0

3213

1045802

39

2311

325.5

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.061 ± 0.079

0.801 ± 0.014

6.124 ± 0.033

5.305 ± 0.041

3.391 ± 0.027

8.55 ± 0.055

2.231 ± 0.022

4.324 ± 0.027

2.796 ± 0.038

10.616 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.017

2.554 ± 0.028

5.214 ± 0.037

3.786 ± 0.028

7.678 ± 0.052

5.315 ± 0.036

4.921 ± 0.035

7.26 ± 0.037

1.516 ± 0.019

2.349 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski