Clostridium leptum CAG:27

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2482 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6P1X5|R6P1X5_9CLOT Putative membrane protein OS=Clostridium leptum CAG:27 OX=1263068 GN=BN578_01953 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.33KK3 pKa = 10.2VLALLLSGALLLSAAACSASPGGEE27 pKa = 3.73SSAGADD33 pKa = 3.16SSSTAEE39 pKa = 3.87EE40 pKa = 4.27SAAGASEE47 pKa = 4.41KK48 pKa = 10.27TSSTDD53 pKa = 3.11GQQLQTITFVLDD65 pKa = 3.13WTPNTNHH72 pKa = 6.24TGIYY76 pKa = 9.8VADD79 pKa = 3.47KK80 pKa = 10.87LGYY83 pKa = 9.65FKK85 pKa = 10.82EE86 pKa = 4.14AGIQIDD92 pKa = 3.63IQQPPEE98 pKa = 4.85DD99 pKa = 3.98GATALVATGKK109 pKa = 10.37AQFGVDD115 pKa = 3.97FQDD118 pKa = 3.84YY119 pKa = 9.59LAPAYY124 pKa = 10.86VSDD127 pKa = 4.43LPVTAVAALIQHH139 pKa = 5.51NTSGIISLKK148 pKa = 10.29EE149 pKa = 3.74KK150 pKa = 10.57GIQSPKK156 pKa = 10.13DD157 pKa = 3.68LEE159 pKa = 4.55GKK161 pKa = 8.45TYY163 pKa = 9.51ATWDD167 pKa = 3.89LPVEE171 pKa = 4.1QATMQNVVEE180 pKa = 4.38NDD182 pKa = 3.21GGDD185 pKa = 3.25WSKK188 pKa = 11.82VNLASVTVTDD198 pKa = 5.15VISALQTNIDD208 pKa = 4.04AVWIYY213 pKa = 8.68YY214 pKa = 10.21AWDD217 pKa = 4.96GIATQVKK224 pKa = 10.2GLDD227 pKa = 3.08TNYY230 pKa = 10.34FYY232 pKa = 10.96FKK234 pKa = 10.44DD235 pKa = 3.43INPVFDD241 pKa = 4.04YY242 pKa = 7.3YY243 pKa = 11.34TPVLVANNDD252 pKa = 3.61YY253 pKa = 11.27LEE255 pKa = 4.3QNPEE259 pKa = 3.67TAKK262 pKa = 11.05AFLAAVAKK270 pKa = 9.8GYY272 pKa = 10.58EE273 pKa = 4.02YY274 pKa = 10.55AIEE277 pKa = 4.31NPEE280 pKa = 3.69EE281 pKa = 3.87AAEE284 pKa = 4.2ILCEE288 pKa = 4.46ADD290 pKa = 3.13PTLDD294 pKa = 3.65SEE296 pKa = 4.96IVLSSQQWLADD307 pKa = 3.49QYY309 pKa = 11.07KK310 pKa = 10.71AEE312 pKa = 4.1VDD314 pKa = 2.59RR315 pKa = 11.84WGYY318 pKa = 10.32IDD320 pKa = 4.0PSRR323 pKa = 11.84WDD325 pKa = 4.88AFYY328 pKa = 10.78QWLWEE333 pKa = 3.96NQLIEE338 pKa = 4.24EE339 pKa = 5.08EE340 pKa = 4.32IPAGFGFSNDD350 pKa = 3.82YY351 pKa = 10.93LPEE354 pKa = 4.04

Molecular weight: 38.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6NCX2|R6NCX2_9CLOT GTPase Der OS=Clostridium leptum CAG:27 OX=1263068 GN=der PE=3 SV=1
MM1 pKa = 7.63VSQQSKK7 pKa = 8.52TIRR10 pKa = 11.84NGKK13 pKa = 8.84KK14 pKa = 8.55EE15 pKa = 3.93RR16 pKa = 11.84TEE18 pKa = 3.29IFYY21 pKa = 10.61INNFIQTDD29 pKa = 4.38RR30 pKa = 11.84IKK32 pKa = 10.95SGSATSQFLSLAINITIFVLLSTYY56 pKa = 7.68SHH58 pKa = 6.28EE59 pKa = 4.27RR60 pKa = 11.84SFLIGYY66 pKa = 8.79RR67 pKa = 11.84RR68 pKa = 11.84AVVRR72 pKa = 11.84NCSPKK77 pKa = 10.65LLGRR81 pKa = 11.84LIRR84 pKa = 11.84KK85 pKa = 8.46GNQKK89 pKa = 9.76QDD91 pKa = 2.75SCFTVIGGRR100 pKa = 11.84SIFPIRR106 pKa = 11.84LCVYY110 pKa = 8.93WRR112 pKa = 11.84HH113 pKa = 6.43LEE115 pKa = 4.04LNNLFPEE122 pKa = 5.1PGDD125 pKa = 3.53PMIMLFQKK133 pKa = 10.69HH134 pKa = 5.67SFPFLKK140 pKa = 10.19RR141 pKa = 11.84RR142 pKa = 11.84NCIAGITNLKK152 pKa = 9.82

Molecular weight: 17.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2482

0

2482

772695

29

4089

311.3

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.402 ± 0.049

1.627 ± 0.026

5.264 ± 0.044

7.082 ± 0.053

4.241 ± 0.044

7.581 ± 0.051

1.677 ± 0.022

6.439 ± 0.048

5.852 ± 0.039

9.553 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.025

3.821 ± 0.035

3.972 ± 0.03

3.657 ± 0.027

4.942 ± 0.052

6.141 ± 0.046

5.123 ± 0.053

7.012 ± 0.037

1.038 ± 0.02

3.762 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski