Streptomyces sparsogenes DSM 40356

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces sparsogenes

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8678 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R1S714|A0A1R1S714_9ACTN Putative NDP-hexose 4-ketoreductase OS=Streptomyces sparsogenes DSM 40356 OX=1331668 GN=SPAR_38679 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84KK3 pKa = 9.57HH4 pKa = 6.53GIIAASTVALALGGALFAAPAAQAATTPVASVVHH38 pKa = 6.58EE39 pKa = 5.29DD40 pKa = 3.72GEE42 pKa = 4.41LWYY45 pKa = 10.62KK46 pKa = 10.36GAAGQTNHH54 pKa = 5.79LTVSEE59 pKa = 4.09AVEE62 pKa = 3.77QRR64 pKa = 11.84GEE66 pKa = 3.78FEE68 pKa = 4.13YY69 pKa = 11.27YY70 pKa = 10.66YY71 pKa = 10.0IITFRR76 pKa = 11.84DD77 pKa = 3.24RR78 pKa = 11.84GDD80 pKa = 3.2IAIDD84 pKa = 3.75PSAATADD91 pKa = 3.43EE92 pKa = 4.2CAYY95 pKa = 9.99PSEE98 pKa = 4.53ADD100 pKa = 3.42HH101 pKa = 6.02TVVRR105 pKa = 11.84CATMAPLGSDD115 pKa = 3.44DD116 pKa = 4.64SDD118 pKa = 4.01NFDD121 pKa = 3.5VDD123 pKa = 5.49LGDD126 pKa = 4.64GDD128 pKa = 4.2DD129 pKa = 4.0TATVDD134 pKa = 4.16ADD136 pKa = 3.37SHH138 pKa = 6.6AYY140 pKa = 9.72SSIYY144 pKa = 9.87GGPGNDD150 pKa = 3.45VIKK153 pKa = 11.03GSARR157 pKa = 11.84DD158 pKa = 3.71VLHH161 pKa = 7.04GGDD164 pKa = 4.02GDD166 pKa = 4.18DD167 pKa = 4.54RR168 pKa = 11.84LDD170 pKa = 3.75GGGGVWSVGPYY181 pKa = 10.66GDD183 pKa = 4.86GGNDD187 pKa = 3.79TITNCAMDD195 pKa = 4.72CYY197 pKa = 10.47GGPGNDD203 pKa = 3.64SMTGGADD210 pKa = 3.17PTDD213 pKa = 2.81NHH215 pKa = 6.72MYY217 pKa = 10.92GEE219 pKa = 4.91DD220 pKa = 3.91GNDD223 pKa = 3.68VIHH226 pKa = 6.63GLSDD230 pKa = 3.38ADD232 pKa = 4.24FIYY235 pKa = 10.5GGRR238 pKa = 11.84GDD240 pKa = 5.0DD241 pKa = 3.14ILYY244 pKa = 10.82GEE246 pKa = 5.09EE247 pKa = 4.69GDD249 pKa = 3.71DD250 pKa = 3.69TIYY253 pKa = 11.3GNSGNDD259 pKa = 3.34TLYY262 pKa = 11.09GGQGKK267 pKa = 7.98DD268 pKa = 3.4TLSGGPGTNKK278 pKa = 9.57VYY280 pKa = 10.87QDD282 pKa = 3.16

Molecular weight: 29.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R1SL80|A0A1R1SL80_9ACTN Ribonuclease VapC OS=Streptomyces sparsogenes DSM 40356 OX=1331668 GN=vapC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8678

0

8678

2800111

25

5956

322.7

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.888 ± 0.037

0.805 ± 0.007

5.97 ± 0.023

5.783 ± 0.025

2.689 ± 0.013

9.614 ± 0.026

2.357 ± 0.014

3.069 ± 0.018

2.032 ± 0.023

10.382 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.686 ± 0.01

1.641 ± 0.013

6.275 ± 0.023

2.608 ± 0.014

8.521 ± 0.03

4.87 ± 0.018

5.953 ± 0.023

8.237 ± 0.022

1.538 ± 0.012

2.081 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski