Pseudomonas benzenivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8EHF4|A0A1G8EHF4_9PSED Uncharacterized protein OS=Pseudomonas benzenivorans OX=556533 GN=SAMN05216603_11244 PE=4 SV=1
MM1 pKa = 6.91HH2 pKa = 7.08TRR4 pKa = 11.84NFFLRR9 pKa = 11.84TLPRR13 pKa = 11.84YY14 pKa = 9.76LLAAALAAGVATTGLYY30 pKa = 10.52AVDD33 pKa = 4.79GSPPGLFEE41 pKa = 6.53LDD43 pKa = 3.61GNTVDD48 pKa = 3.83VQPGGGDD55 pKa = 3.17DD56 pKa = 3.51WGSLYY61 pKa = 11.02AGDD64 pKa = 4.58QLGTPAAFTGILADD78 pKa = 4.26PAPLSIFTQGGSKK91 pKa = 10.43DD92 pKa = 3.66INDD95 pKa = 3.43VTEE98 pKa = 3.89WRR100 pKa = 11.84HH101 pKa = 4.64TDD103 pKa = 3.09GNVPDD108 pKa = 5.32KK109 pKa = 11.45DD110 pKa = 4.98DD111 pKa = 3.37ITNAYY116 pKa = 8.54AAAYY120 pKa = 6.76TVPAGGGDD128 pKa = 3.85SLNDD132 pKa = 3.98PGDD135 pKa = 3.97LIIYY139 pKa = 9.56FGLDD143 pKa = 2.68RR144 pKa = 11.84FANAGDD150 pKa = 3.59AFAGFWFFQDD160 pKa = 3.99QVGLGPNKK168 pKa = 9.95QFIGEE173 pKa = 4.21HH174 pKa = 5.38TEE176 pKa = 3.9GDD178 pKa = 3.78LLVLVEE184 pKa = 4.33YY185 pKa = 9.49PQGANAVPEE194 pKa = 4.11IKK196 pKa = 10.33VYY198 pKa = 10.37QWVASGGDD206 pKa = 3.58VAEE209 pKa = 4.88HH210 pKa = 7.04LDD212 pKa = 4.04QIFEE216 pKa = 4.34TTAQCDD222 pKa = 3.77GLGDD226 pKa = 3.91KK227 pKa = 9.65LACAITNHH235 pKa = 6.85DD236 pKa = 4.22NLSGEE241 pKa = 4.37PAWPYY246 pKa = 8.94TPKK249 pKa = 10.51SGSGLPFEE257 pKa = 4.91SFYY260 pKa = 11.4EE261 pKa = 4.17GGINVSKK268 pKa = 10.97LIGGDD273 pKa = 3.74VPCFSSFLAEE283 pKa = 4.17TRR285 pKa = 11.84SSRR288 pKa = 11.84SEE290 pKa = 3.7TAQLKK295 pKa = 10.84DD296 pKa = 3.66FVLGDD301 pKa = 4.12FDD303 pKa = 4.4LCSIAVTKK311 pKa = 10.75ACTAAVDD318 pKa = 3.88ASDD321 pKa = 4.18GGNSILVDD329 pKa = 3.94FDD331 pKa = 4.23GVVTNDD337 pKa = 3.75GGLDD341 pKa = 3.53LHH343 pKa = 7.04DD344 pKa = 3.81VTVTDD349 pKa = 5.29DD350 pKa = 3.56NGTPGDD356 pKa = 3.75TGDD359 pKa = 3.87DD360 pKa = 3.47VVVFGPADD368 pKa = 3.53LAAGEE373 pKa = 4.24SQPYY377 pKa = 8.81SGSYY381 pKa = 8.37STTAIPATDD390 pKa = 3.19TVTAEE395 pKa = 3.84GHH397 pKa = 6.2RR398 pKa = 11.84NGTSVTATADD408 pKa = 3.66ATCSPDD414 pKa = 3.27IEE416 pKa = 4.52PALTVDD422 pKa = 4.67KK423 pKa = 11.04FCTANINTGGTAIDD437 pKa = 3.5VLFNGTVTNSGNVALEE453 pKa = 4.17DD454 pKa = 3.56VTVVDD459 pKa = 4.84DD460 pKa = 5.29QGTADD465 pKa = 4.05PADD468 pKa = 4.2DD469 pKa = 3.9VTVLGPITLNVGEE482 pKa = 4.35SAPYY486 pKa = 10.04NGGFSVTGSNSSTDD500 pKa = 3.14HH501 pKa = 4.98VTATGSDD508 pKa = 3.87VLTGTPVQANAEE520 pKa = 4.31ATCQADD526 pKa = 4.01VLPAIAVDD534 pKa = 4.45KK535 pKa = 11.33VCTANINAGGTGIDD549 pKa = 3.46VLFGGTVSNTGNVALGDD566 pKa = 3.7VTVVDD571 pKa = 4.32NNGTADD577 pKa = 3.76PADD580 pKa = 4.22DD581 pKa = 3.9VTVLGPITLAPGEE594 pKa = 4.26SAPYY598 pKa = 10.12SGGFSASGSSSTDD611 pKa = 3.01MVVASGTDD619 pKa = 3.63LVTGTPVQADD629 pKa = 3.84ASASCAADD637 pKa = 3.45VLPAIDD643 pKa = 3.73VTKK646 pKa = 10.66QCTDD650 pKa = 2.93AAAFGQAILFDD661 pKa = 3.93GTVTNSGNVALLGVTVVDD679 pKa = 5.25DD680 pKa = 4.43NGTPADD686 pKa = 4.22PSDD689 pKa = 4.34DD690 pKa = 3.56VTFNLGDD697 pKa = 4.04LAPGASANYY706 pKa = 8.86NGSYY710 pKa = 9.58TPSLAGFHH718 pKa = 6.63TNTVVASASDD728 pKa = 3.59AVEE731 pKa = 4.33SGPVSATASATCEE744 pKa = 4.15VPPPPDD750 pKa = 4.17FEE752 pKa = 5.74GCTPGFWKK760 pKa = 10.5NSPGSWVGYY769 pKa = 10.41SPDD772 pKa = 3.46QLVSSVFSLPNGVLANQLGDD792 pKa = 3.86DD793 pKa = 4.16TLMEE797 pKa = 4.51ALGYY801 pKa = 9.82PGGDD805 pKa = 3.28NLVGAAQILLRR816 pKa = 11.84AAVASLLNAAHH827 pKa = 7.44PDD829 pKa = 2.9VDD831 pKa = 4.68FPRR834 pKa = 11.84TEE836 pKa = 3.92AEE838 pKa = 3.99IIADD842 pKa = 3.71VNAALATKK850 pKa = 10.43DD851 pKa = 3.17RR852 pKa = 11.84ATILALASEE861 pKa = 4.85LDD863 pKa = 3.21ADD865 pKa = 4.28NNLGCDD871 pKa = 3.74LANDD875 pKa = 3.6NSFF878 pKa = 3.47

Molecular weight: 89.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8BVY8|A0A1G8BVY8_9PSED UPF0276 protein SAMN05216603_108176 OS=Pseudomonas benzenivorans OX=556533 GN=SAMN05216603_108176 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5186

0

5186

1669955

26

3143

322.0

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.916 ± 0.045

1.056 ± 0.012

5.144 ± 0.025

5.927 ± 0.036

3.577 ± 0.02

8.137 ± 0.036

2.251 ± 0.019

4.486 ± 0.029

3.077 ± 0.031

12.54 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.016

2.661 ± 0.021

4.941 ± 0.026

4.745 ± 0.03

6.904 ± 0.037

5.575 ± 0.028

4.239 ± 0.028

6.763 ± 0.028

1.444 ± 0.017

2.488 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski