Wenling hepe-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KKG8|A0A1L3KKG8_9VIRU Putative glycoprotein OS=Wenling hepe-like virus 2 OX=1923494 PE=4 SV=1
MM1 pKa = 7.39ILSLKK6 pKa = 9.03PLKK9 pKa = 9.48STRR12 pKa = 11.84ICQKK16 pKa = 9.02STLLEE21 pKa = 3.93SVKK24 pKa = 10.61FIEE27 pKa = 4.55SAEE30 pKa = 3.94FVINNFDD37 pKa = 3.57PKK39 pKa = 10.66MNIPAQWVNAANFPRR54 pKa = 11.84KK55 pKa = 9.67GGSAAPYY62 pKa = 10.38VNIANLPDD70 pKa = 5.05DD71 pKa = 4.4LTGQQPSLGDD81 pKa = 4.0ANNLAAMVAILQAARR96 pKa = 11.84YY97 pKa = 8.42KK98 pKa = 10.78ADD100 pKa = 3.63EE101 pKa = 4.58AFSDD105 pKa = 3.89ATAQLQLDD113 pKa = 4.18TVTSDD118 pKa = 3.2EE119 pKa = 4.52LSFVPLVSNVHH130 pKa = 6.14EE131 pKa = 4.22LTPSNFTHH139 pKa = 5.98SWAVSFPPGIKK150 pKa = 9.11QDD152 pKa = 3.68LYY154 pKa = 9.47FTRR157 pKa = 11.84GPTPTGPGQYY167 pKa = 9.98QFYY170 pKa = 10.64NEE172 pKa = 4.36TFVEE176 pKa = 4.69SDD178 pKa = 3.99SGWITQKK185 pKa = 9.74TGSQDD190 pKa = 3.93AIPALPEE197 pKa = 4.21SIQIRR202 pKa = 11.84RR203 pKa = 11.84GGRR206 pKa = 11.84NTFFKK211 pKa = 10.64IKK213 pKa = 9.89GYY215 pKa = 10.85NKK217 pKa = 9.1IRR219 pKa = 11.84LVASTDD225 pKa = 3.17AQQLVTVVCDD235 pKa = 3.77PYY237 pKa = 11.28GVRR240 pKa = 11.84IYY242 pKa = 11.1AGLPFKK248 pKa = 10.59EE249 pKa = 4.41PQLLAQTRR257 pKa = 11.84TVAYY261 pKa = 9.52LRR263 pKa = 11.84NSTVVVGVPGVSEE276 pKa = 3.66ITMIAGYY283 pKa = 8.94VHH285 pKa = 7.12AWADD289 pKa = 3.75TNFSEE294 pKa = 5.61FGFHH298 pKa = 7.62GDD300 pKa = 3.2VEE302 pKa = 4.46KK303 pKa = 10.8CARR306 pKa = 11.84LSALEE311 pKa = 4.09LFYY314 pKa = 10.47TAVRR318 pKa = 11.84AAVGQSVIKK327 pKa = 10.45AALNASSLTQKK338 pKa = 10.29VSALTPALNARR349 pKa = 11.84MVTSLAKK356 pKa = 9.88NAIMGISEE364 pKa = 4.29SFEE367 pKa = 4.27AEE369 pKa = 3.77APFASGQTRR378 pKa = 11.84DD379 pKa = 3.62NAIHH383 pKa = 6.76GVTLVPFGGDD393 pKa = 3.17DD394 pKa = 3.93SDD396 pKa = 4.01PKK398 pKa = 11.01EE399 pKa = 4.03LDD401 pKa = 3.84FILRR405 pKa = 11.84GPIQPFSPWYY415 pKa = 9.21EE416 pKa = 3.98ATSSSGVKK424 pKa = 9.74FRR426 pKa = 11.84TGMVVDD432 pKa = 5.76EE433 pKa = 4.37FAGWSTGTGVGQVTITANHH452 pKa = 6.25EE453 pKa = 4.05SGYY456 pKa = 11.2VNFTPEE462 pKa = 4.72DD463 pKa = 3.3PWNNNLYY470 pKa = 8.97VSKK473 pKa = 10.31RR474 pKa = 11.84VEE476 pKa = 4.0VFLEE480 pKa = 4.18SPTVAGAGFEE490 pKa = 4.3ASGTATYY497 pKa = 10.11KK498 pKa = 10.85GKK500 pKa = 8.97TYY502 pKa = 10.87QLVFDD507 pKa = 4.12VTEE510 pKa = 4.24EE511 pKa = 4.47DD512 pKa = 4.13DD513 pKa = 5.63LITAFASVTLEE524 pKa = 3.9QTHH527 pKa = 6.05FEE529 pKa = 4.06PWVLQNFTTYY539 pKa = 9.73MGTGCSIKK547 pKa = 10.6NQEE550 pKa = 3.42QCYY553 pKa = 10.0IGEE556 pKa = 4.26TEE558 pKa = 4.87HH559 pKa = 8.05IYY561 pKa = 10.77GYY563 pKa = 10.58DD564 pKa = 3.65GPDD567 pKa = 3.39PLMVKK572 pKa = 10.47ALVDD576 pKa = 3.8YY577 pKa = 10.65EE578 pKa = 4.47IGTSDD583 pKa = 3.11STTYY587 pKa = 11.06LHH589 pKa = 6.65RR590 pKa = 11.84CKK592 pKa = 10.45HH593 pKa = 5.74RR594 pKa = 11.84LSPPWNTNLSLEE606 pKa = 4.43TKK608 pKa = 9.98PVINVKK614 pKa = 9.99VYY616 pKa = 10.6SVPPVTAAPVVVDD629 pKa = 3.61TTEE632 pKa = 3.94TIMFKK637 pKa = 10.72EE638 pKa = 4.14LVLTSLNSAVNSVDD652 pKa = 3.37IRR654 pKa = 11.84QQDD657 pKa = 3.13RR658 pKa = 11.84RR659 pKa = 11.84IEE661 pKa = 4.32EE662 pKa = 3.79IEE664 pKa = 3.95RR665 pKa = 11.84RR666 pKa = 11.84TRR668 pKa = 11.84PTVAGFISTAAFTAASFLNPGVGLTAALLVGTVGEE703 pKa = 4.2AVEE706 pKa = 4.68SFQHH710 pKa = 6.87GYY712 pKa = 8.46TIQSIAEE719 pKa = 4.14MVTAGLVHH727 pKa = 6.04TAQRR731 pKa = 11.84RR732 pKa = 11.84VHH734 pKa = 6.56GKK736 pKa = 10.44DD737 pKa = 3.25EE738 pKa = 4.17VDD740 pKa = 3.5EE741 pKa = 5.19SDD743 pKa = 4.21DD744 pKa = 3.82FLVSVLNILLPSKK757 pKa = 10.66ASSVKK762 pKa = 10.22LAEE765 pKa = 4.37HH766 pKa = 6.06RR767 pKa = 11.84TKK769 pKa = 10.89VMKK772 pKa = 10.4SIEE775 pKa = 4.14RR776 pKa = 11.84GEE778 pKa = 4.25PLTVPTAQGFPVGHH792 pKa = 7.06KK793 pKa = 10.21SLSLPFVSLNNKK805 pKa = 9.38QITPIKK811 pKa = 10.25VPLPSEE817 pKa = 4.38VPPKK821 pKa = 9.9FQPYY825 pKa = 10.36ANKK828 pKa = 10.57LEE830 pKa = 4.3NKK832 pKa = 9.51GMYY835 pKa = 9.18PIHH838 pKa = 6.83QSVTANSVMEE848 pKa = 4.49VGDD851 pKa = 3.57QTVGFQISLGVSDD864 pKa = 4.61GVQRR868 pKa = 11.84GDD870 pKa = 3.17GVFPSRR876 pKa = 11.84GGVKK880 pKa = 10.1GVPSEE885 pKa = 4.24PGCSFFLHH893 pKa = 7.38DD894 pKa = 5.12GGISNNVLSIRR905 pKa = 11.84EE906 pKa = 4.03FTSDD910 pKa = 3.04SNIIFHH916 pKa = 6.3EE917 pKa = 4.03NRR919 pKa = 11.84AFMAQLGFRR928 pKa = 11.84KK929 pKa = 9.95SYY931 pKa = 10.48IDD933 pKa = 3.38SLSPEE938 pKa = 4.12RR939 pKa = 11.84MDD941 pKa = 4.84QILKK945 pKa = 10.25IPTVSQMALKK955 pKa = 9.31TVGSEE960 pKa = 3.93RR961 pKa = 11.84CSDD964 pKa = 4.17FSLSLHH970 pKa = 6.25SMASIVQSHH979 pKa = 6.14TGAAGRR985 pKa = 11.84EE986 pKa = 3.76MWGYY990 pKa = 10.8NVLNHH995 pKa = 6.11NCRR998 pKa = 11.84HH999 pKa = 5.27YY1000 pKa = 11.16ARR1002 pKa = 11.84EE1003 pKa = 3.95AFEE1006 pKa = 4.68TIMTGTPQGQVVPWFHH1022 pKa = 7.26RR1023 pKa = 11.84YY1024 pKa = 8.75IKK1026 pKa = 10.33EE1027 pKa = 3.82RR1028 pKa = 11.84ASNNMSRR1035 pKa = 11.84EE1036 pKa = 3.96EE1037 pKa = 3.94FVDD1040 pKa = 3.92VVDD1043 pKa = 5.7NIYY1046 pKa = 10.29QHH1048 pKa = 7.06IYY1050 pKa = 8.34HH1051 pKa = 6.74TLDD1054 pKa = 5.03DD1055 pKa = 4.66IDD1057 pKa = 4.1NSVYY1061 pKa = 10.44DD1062 pKa = 4.14IKK1064 pKa = 11.21HH1065 pKa = 6.84LIATMYY1071 pKa = 11.09GEE1073 pKa = 4.22TLTDD1077 pKa = 3.55EE1078 pKa = 4.46EE1079 pKa = 5.05EE1080 pKa = 4.4EE1081 pKa = 4.27IVDD1084 pKa = 4.09NKK1086 pKa = 10.17YY1087 pKa = 11.14LKK1089 pKa = 10.48DD1090 pKa = 3.65DD1091 pKa = 3.66SKK1093 pKa = 11.48EE1094 pKa = 4.0PRR1096 pKa = 11.84EE1097 pKa = 3.86HH1098 pKa = 6.18TFRR1101 pKa = 11.84NGRR1104 pKa = 11.84PGKK1107 pKa = 10.15LLSFNN1112 pKa = 4.03

Molecular weight: 122.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KKG8|A0A1L3KKG8_9VIRU Putative glycoprotein OS=Wenling hepe-like virus 2 OX=1923494 PE=4 SV=1
MM1 pKa = 7.85ADD3 pKa = 3.36STQEE7 pKa = 3.92STSMAKK13 pKa = 10.45YY14 pKa = 9.74KK15 pKa = 10.69EE16 pKa = 4.39SVSKK20 pKa = 10.76IMNALLHH27 pKa = 6.43FGAILHH33 pKa = 6.76LDD35 pKa = 2.82KK36 pKa = 11.43SMCVFRR42 pKa = 11.84LHH44 pKa = 5.91QNPVYY49 pKa = 10.5RR50 pKa = 11.84IVLKK54 pKa = 9.82ATYY57 pKa = 10.12KK58 pKa = 10.65DD59 pKa = 3.22ASGDD63 pKa = 3.59KK64 pKa = 10.7KK65 pKa = 10.89EE66 pKa = 4.7LEE68 pKa = 4.33TVSWGTKK75 pKa = 8.68SQANHH80 pKa = 5.48YY81 pKa = 10.0ARR83 pKa = 11.84SKK85 pKa = 10.47LLDD88 pKa = 4.16IIFHH92 pKa = 5.85EE93 pKa = 4.25TDD95 pKa = 3.25FVKK98 pKa = 10.59RR99 pKa = 11.84VDD101 pKa = 4.64LEE103 pKa = 4.46AFDD106 pKa = 5.64LLISTKK112 pKa = 10.26RR113 pKa = 11.84RR114 pKa = 11.84LTKK117 pKa = 9.26FASRR121 pKa = 11.84EE122 pKa = 3.85RR123 pKa = 11.84CTCFSLGTRR132 pKa = 11.84RR133 pKa = 11.84HH134 pKa = 6.38HH135 pKa = 5.28YY136 pKa = 8.57TEE138 pKa = 3.74KK139 pKa = 10.27CKK141 pKa = 10.1TFRR144 pKa = 11.84RR145 pKa = 11.84HH146 pKa = 5.1TNFEE150 pKa = 3.93VPLPRR155 pKa = 11.84RR156 pKa = 11.84QDD158 pKa = 3.43YY159 pKa = 9.56PFLPEE164 pKa = 3.57TRR166 pKa = 11.84YY167 pKa = 9.15FAAVAEE173 pKa = 4.69RR174 pKa = 11.84YY175 pKa = 8.82GHH177 pKa = 5.52NHH179 pKa = 4.92QFF181 pKa = 3.33

Molecular weight: 21.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3908

181

2222

977.0

109.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.883 ± 0.405

1.535 ± 0.316

5.962 ± 0.486

5.553 ± 0.358

4.836 ± 0.295

5.604 ± 0.583

2.636 ± 0.243

5.143 ± 0.177

5.732 ± 0.587

7.088 ± 0.375

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.277 ± 0.152

5.425 ± 0.434

5.45 ± 0.314

3.838 ± 0.166

5.118 ± 0.443

7.267 ± 0.428

7.702 ± 0.788

7.293 ± 0.638

0.896 ± 0.06

3.762 ± 0.526
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski