Clostridium sp. CAG:508

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6QCR8|R6QCR8_9CLOT DNA polymerase III subunit gamma/tau OS=Clostridium sp. CAG:508 OX=1262815 GN=dnaX PE=3 SV=1
MM1 pKa = 7.77SDD3 pKa = 4.7LKK5 pKa = 11.15NHH7 pKa = 5.52YY8 pKa = 9.04QAILTEE14 pKa = 4.45LEE16 pKa = 3.82NHH18 pKa = 6.07FQNEE22 pKa = 3.83DD23 pKa = 3.27DD24 pKa = 3.86QAFVLSKK31 pKa = 10.42FQEE34 pKa = 4.03LSMMFMDD41 pKa = 5.21VIDD44 pKa = 4.36RR45 pKa = 11.84LTYY48 pKa = 9.79LTDD51 pKa = 3.05IRR53 pKa = 11.84IKK55 pKa = 10.41EE56 pKa = 4.13VEE58 pKa = 4.31EE59 pKa = 3.88KK60 pKa = 10.32QQEE63 pKa = 3.66IDD65 pKa = 3.52NRR67 pKa = 11.84IEE69 pKa = 4.22TVKK72 pKa = 10.71KK73 pKa = 10.55AVDD76 pKa = 4.4GIEE79 pKa = 3.71NDD81 pKa = 3.08IYY83 pKa = 11.15EE84 pKa = 4.71DD85 pKa = 3.7DD86 pKa = 3.59EE87 pKa = 6.09AYY89 pKa = 9.89EE90 pKa = 4.23FEE92 pKa = 5.56IICPYY97 pKa = 10.16CNHH100 pKa = 6.39EE101 pKa = 4.58FVADD105 pKa = 3.62INSEE109 pKa = 3.91EE110 pKa = 4.12NAEE113 pKa = 4.25VEE115 pKa = 5.03CPEE118 pKa = 3.93CHH120 pKa = 7.25NIIEE124 pKa = 4.51LDD126 pKa = 3.33WNEE129 pKa = 4.03EE130 pKa = 4.21EE131 pKa = 4.72NCGGCCSHH139 pKa = 7.5CDD141 pKa = 3.44GCGEE145 pKa = 4.01EE146 pKa = 5.04DD147 pKa = 5.09DD148 pKa = 5.2EE149 pKa = 5.77SEE151 pKa = 5.22HH152 pKa = 7.67DD153 pKa = 4.17EE154 pKa = 4.24NDD156 pKa = 3.16NDD158 pKa = 3.76EE159 pKa = 4.85DD160 pKa = 3.73MM161 pKa = 5.65

Molecular weight: 18.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6Q5C7|R6Q5C7_9CLOT Fcs2 OS=Clostridium sp. CAG:508 OX=1262815 GN=BN685_00180 PE=4 SV=1
MM1 pKa = 7.3ARR3 pKa = 11.84IAGVDD8 pKa = 3.56LPRR11 pKa = 11.84EE12 pKa = 3.93KK13 pKa = 10.21RR14 pKa = 11.84VEE16 pKa = 3.65IGLTYY21 pKa = 9.99IYY23 pKa = 10.71GIGLTSAQKK32 pKa = 10.17ILAEE36 pKa = 4.22AKK38 pKa = 9.1VNPDD42 pKa = 2.88IRR44 pKa = 11.84VKK46 pKa = 10.9DD47 pKa = 3.65LTDD50 pKa = 3.95DD51 pKa = 3.39QVQAIRR57 pKa = 11.84KK58 pKa = 9.49AMDD61 pKa = 4.09GYY63 pKa = 10.9KK64 pKa = 10.02VEE66 pKa = 4.34GDD68 pKa = 3.46LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.56VALNIKK78 pKa = 9.97RR79 pKa = 11.84LTEE82 pKa = 3.76IGCYY86 pKa = 9.29RR87 pKa = 11.84GLRR90 pKa = 11.84HH91 pKa = 6.72RR92 pKa = 11.84RR93 pKa = 11.84GLPVRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 10.45TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.44GPRR113 pKa = 11.84KK114 pKa = 9.45LVSKK118 pKa = 10.34SKK120 pKa = 10.63KK121 pKa = 9.7

Molecular weight: 13.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1078

0

1078

318357

29

2701

295.3

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.692 ± 0.066

1.092 ± 0.028

5.329 ± 0.051

8.196 ± 0.088

3.812 ± 0.055

5.884 ± 0.064

1.266 ± 0.025

9.745 ± 0.078

9.672 ± 0.074

8.532 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.472 ± 0.034

6.48 ± 0.076

2.794 ± 0.04

3.107 ± 0.038

3.275 ± 0.048

5.501 ± 0.06

5.74 ± 0.077

6.478 ± 0.065

0.62 ± 0.021

4.312 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski