Variovorax sp. PAMC 28711

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Variovorax; unclassified Variovorax

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3960 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126ZF94|A0A126ZF94_9BURK Uncharacterized protein OS=Variovorax sp. PAMC 28711 OX=1795631 GN=AX767_12550 PE=4 SV=1
MM1 pKa = 7.63SYY3 pKa = 10.9YY4 pKa = 10.59VIPAGQAGQLGALGSAHH21 pKa = 7.5LDD23 pKa = 3.6GNLGAFDD30 pKa = 4.5FKK32 pKa = 11.72YY33 pKa = 10.71EE34 pKa = 4.44VIDD37 pKa = 3.45SAYY40 pKa = 9.88GLTPAGSIDD49 pKa = 3.74TVLKK53 pKa = 10.96DD54 pKa = 3.46GTLTLSATPVTDD66 pKa = 3.81AVNLSITGISGAASISDD83 pKa = 3.52EE84 pKa = 4.41VQGDD88 pKa = 3.72DD89 pKa = 4.86ANPDD93 pKa = 3.72TAVVDD98 pKa = 3.87AAGTTVTVNLNIASVDD114 pKa = 3.49YY115 pKa = 11.01DD116 pKa = 3.32GSEE119 pKa = 3.42RR120 pKa = 11.84VMRR123 pKa = 11.84VLIEE127 pKa = 3.96GVPDD131 pKa = 3.96GVTVNAPVNGQAQQVGIGTWVLVFAANALPINAAGGITLGVQFLTGVDD179 pKa = 3.72VAQATHH185 pKa = 7.96PITMTVQTQDD195 pKa = 2.76RR196 pKa = 11.84GAVTSGQAQDD206 pKa = 3.34SVTWNLVTTYY216 pKa = 10.82DD217 pKa = 3.62GAGEE221 pKa = 4.3SLPPTIDD228 pKa = 2.72QWVYY232 pKa = 10.96NGADD236 pKa = 3.51VNEE239 pKa = 4.94DD240 pKa = 3.58LTFSLSDD247 pKa = 3.44VVDD250 pKa = 4.06AQVTVVDD257 pKa = 3.89VSVPNIFTVSLTDD270 pKa = 3.87LPEE273 pKa = 4.39GTQVQGMVLTSVNGVPTWTATVVVPAGANDD303 pKa = 3.8AAVEE307 pKa = 3.9LALEE311 pKa = 4.37GLLNGIQITPPPNSNEE327 pKa = 3.86NNAEE331 pKa = 3.87DD332 pKa = 4.07AFNFNATLTASAQGSFSVSQTIPDD356 pKa = 3.84TVMVIPVAPVTDD368 pKa = 3.53AAVITVSAVDD378 pKa = 3.39AGEE381 pKa = 4.18NPVDD385 pKa = 3.68GSVSVTIDD393 pKa = 3.22VRR395 pKa = 11.84SNPVDD400 pKa = 3.28GSNGVIVGGLLYY412 pKa = 10.65VQVSATGGGNAGGTLTYY429 pKa = 10.27QGNPAALTPVTNPPGVPAGTYY450 pKa = 9.13YY451 pKa = 10.98LVPVGPSGDD460 pKa = 3.91SVNLVYY466 pKa = 10.29TPPSGTSPGNVIFTALANTQEE487 pKa = 3.97TGAAVVTGSASDD499 pKa = 3.39TATISAVNNGVTVTGFSTPVSAPEE523 pKa = 3.92TDD525 pKa = 3.49GSGLGTAIALPGLMVNLIDD544 pKa = 4.33SDD546 pKa = 3.76GSEE549 pKa = 4.41SIKK552 pKa = 10.83SVTLAGVPVGFLVYY566 pKa = 10.56VGGQLATNAGGNGTSNTWVISNSGTFGEE594 pKa = 4.66VKK596 pKa = 10.05IAPPAHH602 pKa = 6.73WSGEE606 pKa = 4.12LNGLKK611 pKa = 10.36LVVEE615 pKa = 4.49SGEE618 pKa = 4.04NLLAKK623 pKa = 10.35SLEE626 pKa = 4.16EE627 pKa = 3.8FLLPSVTVTPVASGLTLDD645 pKa = 3.51TTQAVGRR652 pKa = 11.84EE653 pKa = 4.05GGIIGLNLNASMTDD667 pKa = 3.38PVAATSTLPDD677 pKa = 3.43SSTEE681 pKa = 4.06TVTVQFKK688 pKa = 11.25GLGEE692 pKa = 3.93YY693 pKa = 10.49AAFYY697 pKa = 10.95VDD699 pKa = 4.04STLLTDD705 pKa = 4.16SGTSGHH711 pKa = 6.45TIGYY715 pKa = 9.78NDD717 pKa = 3.98LTDD720 pKa = 3.65TYY722 pKa = 9.74TITGLTQVEE731 pKa = 4.06MDD733 pKa = 3.3QLGFKK738 pKa = 9.69QAASALVDD746 pKa = 3.37QDD748 pKa = 3.7GAAGTQIEE756 pKa = 4.55VTAWTVEE763 pKa = 4.26SGGGPISAVEE773 pKa = 4.03TADD776 pKa = 2.83ITVNMTVVQPTSGADD791 pKa = 3.24SFIWGGEE798 pKa = 3.67GKK800 pKa = 10.55AIINGAAGDD809 pKa = 3.85DD810 pKa = 3.94TVALRR815 pKa = 11.84LGEE818 pKa = 4.38DD819 pKa = 3.32VSGANLAMGLRR830 pKa = 11.84NIEE833 pKa = 4.08VLDD836 pKa = 4.61LSAQGEE842 pKa = 4.31NAVTNLTATDD852 pKa = 3.76VLSLTGSNHH861 pKa = 6.24ILTINGTGDD870 pKa = 3.87DD871 pKa = 3.99SVQLGGGASAWTAGASSGGYY891 pKa = 8.01TDD893 pKa = 3.69YY894 pKa = 11.35TSGAGASLVTVRR906 pKa = 11.84IDD908 pKa = 3.98DD909 pKa = 5.61DD910 pKa = 3.57ITHH913 pKa = 6.65SYY915 pKa = 8.28VV916 pKa = 2.94

Molecular weight: 92.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126ZCD0|A0A126ZCD0_9BURK LuxR family transcriptional regulator OS=Variovorax sp. PAMC 28711 OX=1795631 GN=AX767_06830 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 9.71QASKK9 pKa = 9.04VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3960

0

3960

1274502

32

3480

321.8

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.172 ± 0.053

0.845 ± 0.011

5.384 ± 0.032

4.989 ± 0.036

3.612 ± 0.026

8.516 ± 0.05

2.144 ± 0.022

4.446 ± 0.026

3.384 ± 0.042

10.47 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.018

2.626 ± 0.026

5.207 ± 0.029

3.669 ± 0.026

6.698 ± 0.044

5.325 ± 0.027

5.491 ± 0.035

7.928 ± 0.031

1.451 ± 0.018

2.135 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski