Flavobacteriaceae bacterium PLHSN227

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2578 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A507ZBM8|A0A507ZBM8_9FLAO SDR family oxidoreductase OS=Flavobacteriaceae bacterium PLHSN227 OX=2576776 GN=FKR84_12620 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 9.25NTLLLLAFLSTIGTFAQVNANDD24 pKa = 4.1ANLLVCEE31 pKa = 4.74EE32 pKa = 4.46DD33 pKa = 3.19TDD35 pKa = 4.75GYY37 pKa = 11.09ATFDD41 pKa = 3.24LTQANTAILNGQSPADD57 pKa = 4.03LEE59 pKa = 4.18VSYY62 pKa = 11.55YY63 pKa = 8.81STEE66 pKa = 3.72VDD68 pKa = 4.23AEE70 pKa = 4.44SATNPLMPTYY80 pKa = 10.97SNVIPFTEE88 pKa = 3.98TLYY91 pKa = 10.81TRR93 pKa = 11.84VEE95 pKa = 4.3NIGTGNFATGLLVLEE110 pKa = 4.56VGEE113 pKa = 4.61AFQILQDD120 pKa = 3.42IPDD123 pKa = 4.18NVACDD128 pKa = 3.74QDD130 pKa = 4.35DD131 pKa = 3.91EE132 pKa = 4.81PMDD135 pKa = 5.39GITQFNLTTAEE146 pKa = 4.09SLILGDD152 pKa = 5.01LNPANHH158 pKa = 6.39SFHH161 pKa = 7.14YY162 pKa = 10.74YY163 pKa = 8.66LTEE166 pKa = 5.15DD167 pKa = 3.88DD168 pKa = 5.33AEE170 pKa = 4.16NAINEE175 pKa = 4.18ITDD178 pKa = 3.54PEE180 pKa = 4.52NFTNIYY186 pKa = 10.19NPITIFVMVKK196 pKa = 10.32NEE198 pKa = 4.22SGCTAMSSFEE208 pKa = 4.95LYY210 pKa = 10.16PSGCWEE216 pKa = 5.19DD217 pKa = 3.59YY218 pKa = 11.53DD219 pKa = 4.34NDD221 pKa = 4.13LVSGADD227 pKa = 3.03EE228 pKa = 4.42DD229 pKa = 4.78VNNNGNLNDD238 pKa = 5.46DD239 pKa = 4.2DD240 pKa = 4.42TDD242 pKa = 4.64GDD244 pKa = 4.03GLKK247 pKa = 10.84NYY249 pKa = 8.84MDD251 pKa = 4.86NDD253 pKa = 4.18DD254 pKa = 5.34DD255 pKa = 6.03GDD257 pKa = 4.78GILTADD263 pKa = 3.96EE264 pKa = 5.2DD265 pKa = 4.96YY266 pKa = 11.41NGNGDD271 pKa = 3.89PTDD274 pKa = 5.15DD275 pKa = 4.27DD276 pKa = 4.18TDD278 pKa = 3.8NSDD281 pKa = 3.31VADD284 pKa = 3.76YY285 pKa = 11.37LEE287 pKa = 4.79EE288 pKa = 4.16NVALATTSYY297 pKa = 10.12TKK299 pKa = 10.65NQFSVYY305 pKa = 9.79PNPAKK310 pKa = 10.16EE311 pKa = 3.85AFFIEE316 pKa = 4.48FTKK319 pKa = 10.84AEE321 pKa = 4.29KK322 pKa = 10.41SRR324 pKa = 11.84SLQIFTFAGKK334 pKa = 8.93MIKK337 pKa = 9.96EE338 pKa = 3.77ISFEE342 pKa = 4.06NSVKK346 pKa = 10.26KK347 pKa = 10.09QRR349 pKa = 11.84ISITDD354 pKa = 4.11FPAGIYY360 pKa = 9.46FLRR363 pKa = 11.84LNTAEE368 pKa = 3.98NTGVRR373 pKa = 11.84KK374 pKa = 10.17LIVRR378 pKa = 4.36

Molecular weight: 41.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A507ZTZ1|A0A507ZTZ1_9FLAO Uncharacterized protein OS=Flavobacteriaceae bacterium PLHSN227 OX=2576776 GN=FKR84_00055 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.05GFRR19 pKa = 11.84EE20 pKa = 4.13RR21 pKa = 11.84MSSVNGRR28 pKa = 11.84KK29 pKa = 9.11VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2578

0

2578

856065

24

3453

332.1

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.768 ± 0.038

0.69 ± 0.015

5.271 ± 0.043

7.159 ± 0.055

5.242 ± 0.043

6.109 ± 0.043

1.709 ± 0.02

7.505 ± 0.044

8.464 ± 0.07

9.58 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.025

6.115 ± 0.054

3.423 ± 0.028

3.886 ± 0.029

3.461 ± 0.027

5.994 ± 0.036

5.466 ± 0.051

6.025 ± 0.033

0.986 ± 0.017

4.087 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski