Clostridium sp. CAG:914

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7M6X5|R7M6X5_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:914 OX=1262846 GN=BN809_01222 PE=4 SV=1
MM1 pKa = 6.56TTIISIVLLIILVSASTYY19 pKa = 10.39AVVVNVKK26 pKa = 9.85EE27 pKa = 4.1NNEE30 pKa = 3.59VLEE33 pKa = 4.22IVNEE37 pKa = 3.96VNIIDD42 pKa = 5.26LVTDD46 pKa = 3.22NDD48 pKa = 3.78GVYY51 pKa = 11.04NNTYY55 pKa = 9.25YY56 pKa = 11.0NVRR59 pKa = 11.84NEE61 pKa = 3.94LDD63 pKa = 3.37ITDD66 pKa = 4.34SEE68 pKa = 4.3ASIIIQSDD76 pKa = 3.77SLNEE80 pKa = 3.9ALHH83 pKa = 6.69LVLQSLVDD91 pKa = 3.56YY92 pKa = 10.59KK93 pKa = 11.0INKK96 pKa = 8.64NNSARR101 pKa = 11.84LSNQEE106 pKa = 4.19LYY108 pKa = 11.3NLILEE113 pKa = 4.42YY114 pKa = 10.44TNKK117 pKa = 10.57DD118 pKa = 3.1EE119 pKa = 6.08AMPSYY124 pKa = 10.82LKK126 pKa = 10.86DD127 pKa = 3.85KK128 pKa = 10.81IIDD131 pKa = 3.45KK132 pKa = 10.78SNIYY136 pKa = 8.33ITDD139 pKa = 3.6VNDD142 pKa = 3.52FLYY145 pKa = 10.66DD146 pKa = 3.43IEE148 pKa = 4.3VSYY151 pKa = 11.08LGEE154 pKa = 3.96LL155 pKa = 3.65

Molecular weight: 17.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7M830|R7M830_9CLOT Channel protein hemolysin III family OS=Clostridium sp. CAG:914 OX=1262846 GN=BN809_00989 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.39QPNNRR10 pKa = 11.84KK11 pKa = 9.04KK12 pKa = 10.09AKK14 pKa = 9.55KK15 pKa = 8.93HH16 pKa = 4.93GFFARR21 pKa = 11.84MKK23 pKa = 10.77SNIINKK29 pKa = 8.98RR30 pKa = 11.84RR31 pKa = 11.84AKK33 pKa = 10.32KK34 pKa = 9.11RR35 pKa = 11.84TRR37 pKa = 11.84LSAA40 pKa = 3.64

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1262

0

1262

371395

30

2731

294.3

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.756 ± 0.057

1.104 ± 0.027

6.201 ± 0.061

6.967 ± 0.079

4.098 ± 0.057

5.199 ± 0.081

1.23 ± 0.023

10.898 ± 0.104

9.757 ± 0.093

8.971 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.031

8.132 ± 0.099

2.374 ± 0.04

1.982 ± 0.034

3.112 ± 0.052

6.426 ± 0.059

5.272 ± 0.08

5.867 ± 0.063

0.546 ± 0.018

5.675 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski