Variovorax paradoxus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Variovorax

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6788 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H2LW41|A0A0H2LW41_VARPD Multidrug resistance protein MdtB OS=Variovorax paradoxus OX=34073 GN=mdtB PE=4 SV=1
MM1 pKa = 6.94EE2 pKa = 5.37AVYY5 pKa = 10.6KK6 pKa = 10.85SGGASRR12 pKa = 11.84KK13 pKa = 9.22AAADD17 pKa = 3.66TPLVLDD23 pKa = 4.13RR24 pKa = 11.84PSIVSLKK31 pKa = 10.04IAPEE35 pKa = 3.99SVLKK39 pKa = 9.89FEE41 pKa = 4.98RR42 pKa = 11.84RR43 pKa = 11.84GGDD46 pKa = 3.25LVLVLQGGQEE56 pKa = 3.97VAVRR60 pKa = 11.84GFFTEE65 pKa = 4.1YY66 pKa = 10.84SDD68 pKa = 4.27GGRR71 pKa = 11.84NDD73 pKa = 4.32LVLEE77 pKa = 4.93DD78 pKa = 4.05GAGVQWWGQYY88 pKa = 6.1TAPWKK93 pKa = 10.3DD94 pKa = 3.47FHH96 pKa = 6.19FTEE99 pKa = 5.48IEE101 pKa = 3.92WTDD104 pKa = 3.07AGAALLPDD112 pKa = 4.84GVPGWLLGALGVLGVGAAASGGGGGGGGGGGPAFIPPLLPPQNRR156 pKa = 11.84GPEE159 pKa = 4.25GKK161 pKa = 9.84AEE163 pKa = 3.96PVATEE168 pKa = 3.49QDD170 pKa = 3.58KK171 pKa = 10.98PISGQVQATDD181 pKa = 3.77PDD183 pKa = 4.34RR184 pKa = 11.84DD185 pKa = 3.86TLNFTVAKK193 pKa = 9.39GPEE196 pKa = 4.27HH197 pKa = 5.83GTVTVDD203 pKa = 3.39PSTGQFLYY211 pKa = 10.18TPNPGYY217 pKa = 10.3EE218 pKa = 4.32GPDD221 pKa = 3.33SFEE224 pKa = 4.12VTVSDD229 pKa = 4.0GRR231 pKa = 11.84GGTTTVKK238 pKa = 10.54VPVTVSPVNDD248 pKa = 3.66APTAPDD254 pKa = 3.31YY255 pKa = 11.63TEE257 pKa = 4.2TTNEE261 pKa = 3.95DD262 pKa = 3.81TPVSGRR268 pKa = 11.84VTGSDD273 pKa = 3.2MDD275 pKa = 4.02SGDD278 pKa = 3.13TLTYY282 pKa = 11.07VKK284 pKa = 10.65GSDD287 pKa = 3.65PAHH290 pKa = 5.77GTVTVNPDD298 pKa = 2.63GTYY301 pKa = 10.05TYY303 pKa = 10.47TPNPDD308 pKa = 3.37FNGTDD313 pKa = 3.24SFTVTVSDD321 pKa = 3.49GHH323 pKa = 6.56GGTTTSTVTVTINPVNDD340 pKa = 3.1VPVFVDD346 pKa = 4.23GGGTPVNTTDD356 pKa = 4.08GYY358 pKa = 11.1VFGYY362 pKa = 10.61DD363 pKa = 3.27EE364 pKa = 4.79NRR366 pKa = 11.84PAGAVLGTVRR376 pKa = 11.84ATDD379 pKa = 3.22VDD381 pKa = 4.46SATVTYY387 pKa = 10.61SILSGNDD394 pKa = 2.77NGYY397 pKa = 9.62FAIDD401 pKa = 3.76PVTGEE406 pKa = 4.14ISLTPAGAAAFVNDD420 pKa = 3.89YY421 pKa = 10.49EE422 pKa = 5.65AGANAHH428 pKa = 5.79SLVVGANDD436 pKa = 3.74GTVTTSIPVTLNEE449 pKa = 4.18QNVNDD454 pKa = 4.63APTAPGDD461 pKa = 3.78TKK463 pKa = 9.37TTDD466 pKa = 2.82EE467 pKa = 4.47DD468 pKa = 4.64TPVSGQIVGTDD479 pKa = 3.11VDD481 pKa = 5.05GDD483 pKa = 3.72TLTYY487 pKa = 11.08VKK489 pKa = 10.65GSDD492 pKa = 3.65PAHH495 pKa = 5.68GTVTVNADD503 pKa = 2.83GTYY506 pKa = 9.88IYY508 pKa = 10.64TPGANFNGTDD518 pKa = 3.4SFTVTVSDD526 pKa = 3.49GHH528 pKa = 6.56GGTTTSTITVTVDD541 pKa = 3.27PVNDD545 pKa = 3.43APMVPDD551 pKa = 3.38YY552 pKa = 11.22TKK554 pKa = 8.55TTNEE558 pKa = 4.05DD559 pKa = 3.86TPVSGQVVGSDD570 pKa = 3.23VDD572 pKa = 4.34GDD574 pKa = 3.71TLTYY578 pKa = 11.02VKK580 pKa = 10.67GSDD583 pKa = 3.66PSHH586 pKa = 5.77GTVTVNADD594 pKa = 3.0GTYY597 pKa = 9.86TYY599 pKa = 11.45VPGANFNGTDD609 pKa = 3.4SFTVTVSDD617 pKa = 3.49GHH619 pKa = 6.56GGTTTSTVTVTINPVNDD636 pKa = 3.1VPVFVDD642 pKa = 4.23GGGTPVNTTDD652 pKa = 4.08GYY654 pKa = 11.1VFGYY658 pKa = 10.61DD659 pKa = 3.27EE660 pKa = 4.79NRR662 pKa = 11.84PAGAVLGTVRR672 pKa = 11.84ATDD675 pKa = 3.22VDD677 pKa = 4.46SATVTYY683 pKa = 10.61SILSGNDD690 pKa = 2.77NGYY693 pKa = 9.62FAIDD697 pKa = 3.76PVTGEE702 pKa = 4.14ISLTPAGAAAFVNDD716 pKa = 3.89YY717 pKa = 10.49EE718 pKa = 5.65AGANAHH724 pKa = 5.79SLVVGANDD732 pKa = 3.61GTVTTNIPVTLNEE745 pKa = 3.97QNINDD750 pKa = 4.58APTAPDD756 pKa = 3.34DD757 pKa = 3.95TKK759 pKa = 9.39TTNEE763 pKa = 4.07DD764 pKa = 3.84TPVSGQVTGSDD775 pKa = 3.34VDD777 pKa = 4.15GDD779 pKa = 3.91TLTYY783 pKa = 11.0AKK785 pKa = 10.34GSNPSHH791 pKa = 5.83GTVTVNADD799 pKa = 3.0GTYY802 pKa = 9.89TYY804 pKa = 11.45VPGPNFNGTDD814 pKa = 3.42SFTVTVSDD822 pKa = 3.49GHH824 pKa = 6.56GGTTTSTVNVTVNPVNDD841 pKa = 3.86APSATVNNAGVVSEE855 pKa = 4.45GSLPGGIPAAGEE867 pKa = 4.16PPLQSTGKK875 pKa = 10.21ISIADD880 pKa = 3.87PDD882 pKa = 4.0SAAGDD887 pKa = 3.8LSVSLSGPNGVTSGGQPVSWTWDD910 pKa = 3.26AGTHH914 pKa = 5.07TLTGSVTVGGVTTEE928 pKa = 3.83VMTVAVGNVTATGAGQFEE946 pKa = 4.58AGYY949 pKa = 8.04TVTLKK954 pKa = 11.09APIDD958 pKa = 3.73HH959 pKa = 6.93LPGNGEE965 pKa = 4.19GVSNLHH971 pKa = 6.29FEE973 pKa = 4.55AVVSDD978 pKa = 4.18GQASGAPVGFEE989 pKa = 4.27VPVKK993 pKa = 10.7DD994 pKa = 4.51DD995 pKa = 4.22APVLVNGEE1003 pKa = 4.07QAVDD1007 pKa = 3.48VAPIDD1012 pKa = 3.8TNLMVILDD1020 pKa = 4.33LSGSMGQEE1028 pKa = 3.64TPTRR1032 pKa = 11.84LSRR1035 pKa = 11.84AKK1037 pKa = 10.22EE1038 pKa = 4.12AIQNLIDD1045 pKa = 5.05GYY1047 pKa = 11.03DD1048 pKa = 3.12LYY1050 pKa = 11.8GDD1052 pKa = 3.68VRR1054 pKa = 11.84VQLVTFSTTGASQQAWMTAAEE1075 pKa = 4.42AKK1077 pKa = 10.75ALVQNLQASGSTNYY1091 pKa = 10.25DD1092 pKa = 3.03AALAAAMNGFSATGKK1107 pKa = 10.45LDD1109 pKa = 3.35GAQNVSYY1116 pKa = 10.17FLTDD1120 pKa = 3.7GEE1122 pKa = 4.4PTLGDD1127 pKa = 3.66GNTEE1131 pKa = 3.77QLANSSNSSTADD1143 pKa = 3.09RR1144 pKa = 11.84GIQAGEE1150 pKa = 3.57EE1151 pKa = 4.22AIWTNFLSTHH1161 pKa = 5.96QINSFALGLGSSLNAAAQAFIDD1183 pKa = 4.68PIAYY1187 pKa = 10.11NGNTGANTNGQIVTDD1202 pKa = 4.16TSQLNDD1208 pKa = 3.63MLQGTISVPPTVSNLLTGGLGGSSGFGADD1237 pKa = 3.36GGHH1240 pKa = 6.39VSTLSIDD1247 pKa = 3.45GTTYY1251 pKa = 11.35AFDD1254 pKa = 3.62SSTGLMTKK1262 pKa = 9.44TGPATGSDD1270 pKa = 3.75YY1271 pKa = 11.19SYY1273 pKa = 11.5NAATHH1278 pKa = 5.27QVTIATAQGGKK1289 pKa = 10.03LVVDD1293 pKa = 4.65FDD1295 pKa = 4.3SGEE1298 pKa = 3.84FSYY1301 pKa = 10.89QVAPRR1306 pKa = 11.84TSGSHH1311 pKa = 6.42NYY1313 pKa = 10.52DD1314 pKa = 3.02EE1315 pKa = 4.48TLSYY1319 pKa = 10.76QVVDD1323 pKa = 3.87RR1324 pKa = 11.84DD1325 pKa = 3.72GDD1327 pKa = 3.73ASNLATQTLHH1337 pKa = 6.57VNYY1340 pKa = 9.43TPAAGAAGAAALFSSADD1357 pKa = 3.6SLGLAGVDD1365 pKa = 3.33EE1366 pKa = 4.64ATVSLEE1372 pKa = 3.82QQQPQDD1378 pKa = 3.46APHH1381 pKa = 6.72FPFGDD1386 pKa = 4.12SPWGGTQLSDD1396 pKa = 3.38MLHH1399 pKa = 6.96AYY1401 pKa = 9.25NAAEE1405 pKa = 4.04SLQVLLQAAFPPAFASTQASPTVLALASSADD1436 pKa = 3.59SLTAYY1441 pKa = 9.61APPVAPSLDD1450 pKa = 4.22DD1451 pKa = 3.89EE1452 pKa = 4.71LHH1454 pKa = 6.08MGLALHH1460 pKa = 6.83HH1461 pKa = 6.68

Molecular weight: 148.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H2M5N9|A0A0H2M5N9_VARPD Alpha-D-glucose-1-phosphatase OS=Variovorax paradoxus OX=34073 GN=VPARA_11970 PE=4 SV=1
MM1 pKa = 7.71PPSKK5 pKa = 10.42SPPSRR10 pKa = 11.84SPPSRR15 pKa = 11.84LPTRR19 pKa = 11.84PSSPNRR25 pKa = 11.84PPSKK29 pKa = 9.58PSSPSTPPSRR39 pKa = 11.84PSSPSRR45 pKa = 11.84LSTNPSSPSTPPSRR59 pKa = 11.84PSSARR64 pKa = 11.84RR65 pKa = 11.84LPTAPPLPSKK75 pKa = 10.12PSSPSRR81 pKa = 11.84PPTKK85 pKa = 9.62PSSPSTPPSRR95 pKa = 11.84PSSPSTPSVRR105 pKa = 11.84PPSRR109 pKa = 11.84LSSPPSNPPSGLSSSRR125 pKa = 11.84PLTAPSAPRR134 pKa = 11.84RR135 pKa = 11.84LSTRR139 pKa = 11.84PSWPSRR145 pKa = 11.84PPEE148 pKa = 4.4RR149 pKa = 11.84PSLPNKK155 pKa = 9.16PDD157 pKa = 3.6SAPSPPSRR165 pKa = 11.84PPTTPSPPSRR175 pKa = 11.84LPTGLPSPIRR185 pKa = 11.84PPSRR189 pKa = 11.84PSSPSTPPSTPPRR202 pKa = 11.84APSSPSRR209 pKa = 11.84PPTMPSSPITLSSRR223 pKa = 11.84LPMALPSPMRR233 pKa = 11.84PSSAPPSASKK243 pKa = 10.65PPTRR247 pKa = 11.84PSSPTSPARR256 pKa = 11.84RR257 pKa = 11.84LSWPSRR263 pKa = 11.84PSTTLPTTPSSPTRR277 pKa = 11.84PSRR280 pKa = 11.84APSRR284 pKa = 11.84PRR286 pKa = 11.84LPVRR290 pKa = 11.84PLTMPSSPTRR300 pKa = 11.84LSSPFCRR307 pKa = 11.84ASVTT311 pKa = 3.52

Molecular weight: 32.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6788

0

6788

2201532

29

3308

324.3

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.322 ± 0.047

0.911 ± 0.01

5.096 ± 0.023

5.251 ± 0.026

3.552 ± 0.017

8.536 ± 0.03

2.221 ± 0.013

4.327 ± 0.019

3.178 ± 0.022

10.575 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.016

2.516 ± 0.017

5.441 ± 0.022

3.69 ± 0.019

7.052 ± 0.029

5.448 ± 0.022

5.082 ± 0.022

7.688 ± 0.024

1.45 ± 0.012

2.17 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski