Streptomyces phage Amela

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Camvirus; Streptomyces virus Amela

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1Y9K9|A0A0K1Y9K9_9CAUD Minor tail protein OS=Streptomyces phage Amela OX=1673877 GN=SEA_AMELA_22 PE=4 SV=1
MM1 pKa = 8.2PYY3 pKa = 10.32RR4 pKa = 11.84HH5 pKa = 6.23VLPAMNRR12 pKa = 11.84AHH14 pKa = 7.22GDD16 pKa = 3.32PYY18 pKa = 11.65SFITEE23 pKa = 4.27DD24 pKa = 3.3GTIEE28 pKa = 4.62FVFDD32 pKa = 3.85LEE34 pKa = 4.8NFDD37 pKa = 3.89FHH39 pKa = 8.7IDD41 pKa = 3.19ATFGYY46 pKa = 10.01SPSRR50 pKa = 11.84MNAVLQQAEE59 pKa = 4.06WWGLEE64 pKa = 3.58LMPYY68 pKa = 9.32SEE70 pKa = 5.53CDD72 pKa = 3.07VDD74 pKa = 5.23FLPGGITRR82 pKa = 11.84IYY84 pKa = 8.32LTPIVPVEE92 pKa = 3.92VAEE95 pKa = 4.28AEE97 pKa = 4.36MVRR100 pKa = 11.84EE101 pKa = 4.09LLAEE105 pKa = 4.04VDD107 pKa = 4.04ALTVTPLDD115 pKa = 4.22DD116 pKa = 3.91SLEE119 pKa = 4.23EE120 pKa = 4.2VPSDD124 pKa = 4.04PEE126 pKa = 3.91LHH128 pKa = 6.9GDD130 pKa = 3.83PEE132 pKa = 4.23QGAPEE137 pKa = 4.01QLGAAA142 pKa = 4.33

Molecular weight: 15.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1Y9K2|A0A0K1Y9K2_9CAUD Uncharacterized protein OS=Streptomyces phage Amela OX=1673877 GN=SEA_AMELA_45 PE=4 SV=1
MM1 pKa = 6.84SHH3 pKa = 5.45QRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GQSAKK12 pKa = 8.92IWKK15 pKa = 9.59SKK17 pKa = 10.0LVEE20 pKa = 3.93DD21 pKa = 4.27RR22 pKa = 11.84RR23 pKa = 11.84GNKK26 pKa = 9.78LLTADD31 pKa = 3.71ADD33 pKa = 4.15GPHH36 pKa = 5.82VVRR39 pKa = 11.84AAFIPQRR46 pKa = 11.84SAKK49 pKa = 10.65AEE51 pKa = 4.01VPGQQLINITRR62 pKa = 11.84MIVAADD68 pKa = 4.06LEE70 pKa = 5.08DD71 pKa = 3.4VTLWSRR77 pKa = 11.84VEE79 pKa = 3.87YY80 pKa = 10.34QGRR83 pKa = 11.84QWDD86 pKa = 4.06IVSPPAYY93 pKa = 9.52HH94 pKa = 6.62HH95 pKa = 6.66GPRR98 pKa = 11.84KK99 pKa = 7.28TRR101 pKa = 11.84HH102 pKa = 4.0WSIDD106 pKa = 2.83IRR108 pKa = 11.84EE109 pKa = 4.17RR110 pKa = 11.84TT111 pKa = 3.47

Molecular weight: 12.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15045

32

1370

200.6

21.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.987 ± 0.479

0.698 ± 0.112

6.441 ± 0.255

6.514 ± 0.498

2.984 ± 0.177

8.681 ± 0.37

1.834 ± 0.201

4.46 ± 0.348

4.6 ± 0.335

8.242 ± 0.441

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.167 ± 0.145

3.018 ± 0.168

4.978 ± 0.227

3.303 ± 0.149

6.261 ± 0.481

6.009 ± 0.249

6.78 ± 0.295

7.212 ± 0.307

1.874 ± 0.143

2.958 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski