Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Idiomarinaceae; Idiomarina; Idiomarina loihiensis

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2608 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5QZG3|Q5QZG3_IDILO Possible Zn-finger protein OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) OX=283942 GN=IL2600 PE=4 SV=1
MM1 pKa = 7.86FYY3 pKa = 10.39RR4 pKa = 11.84NCFAASLLVLACSQLTACNSDD25 pKa = 3.31SSSEE29 pKa = 4.21SSDD32 pKa = 3.33HH33 pKa = 6.01QEE35 pKa = 3.98SGIWQPKK42 pKa = 9.32PGTSWHH48 pKa = 5.94WQLEE52 pKa = 4.4NYY54 pKa = 9.93DD55 pKa = 4.16NLDD58 pKa = 3.16ISKK61 pKa = 10.4DD62 pKa = 3.46AEE64 pKa = 4.13AFDD67 pKa = 3.83IDD69 pKa = 4.5LFEE72 pKa = 4.47GAEE75 pKa = 4.31GGDD78 pKa = 3.73DD79 pKa = 4.91SIISSLKK86 pKa = 10.54DD87 pKa = 2.88NGKK90 pKa = 9.74RR91 pKa = 11.84VICYY95 pKa = 8.37FSAGTRR101 pKa = 11.84EE102 pKa = 4.19DD103 pKa = 3.26WRR105 pKa = 11.84PDD107 pKa = 2.86ATEE110 pKa = 3.91FSEE113 pKa = 5.34DD114 pKa = 3.17AVIANGEE121 pKa = 4.03MADD124 pKa = 3.5WPGEE128 pKa = 3.9VWLDD132 pKa = 3.45INNEE136 pKa = 3.71AVLNEE141 pKa = 4.11NIKK144 pKa = 10.57PIMEE148 pKa = 4.43ARR150 pKa = 11.84LDD152 pKa = 3.8LAQSAGCDD160 pKa = 3.03AVEE163 pKa = 4.38PDD165 pKa = 4.54NVDD168 pKa = 3.28GYY170 pKa = 10.95INTDD174 pKa = 3.19EE175 pKa = 4.3TKK177 pKa = 11.06GIITYY182 pKa = 10.6DD183 pKa = 3.41DD184 pKa = 3.34QLNYY188 pKa = 10.95NKK190 pKa = 9.73WLANAAHH197 pKa = 6.33SRR199 pKa = 11.84GLSIGLKK206 pKa = 10.24NDD208 pKa = 2.84VDD210 pKa = 3.93QLNEE214 pKa = 4.07LVNDD218 pKa = 3.79FDD220 pKa = 4.47FAVNEE225 pKa = 3.67QCYY228 pKa = 10.04AYY230 pKa = 10.5GNEE233 pKa = 4.12CVSYY237 pKa = 11.22EE238 pKa = 4.39DD239 pKa = 4.71TFLKK243 pKa = 10.51NNKK246 pKa = 9.19AVFVQEE252 pKa = 4.13YY253 pKa = 9.9YY254 pKa = 10.8EE255 pKa = 4.96DD256 pKa = 4.16GSEE259 pKa = 4.6GEE261 pKa = 3.95ISQQEE266 pKa = 4.38FEE268 pKa = 5.44SNACSYY274 pKa = 10.61FLDD277 pKa = 3.84VGISALWKK285 pKa = 10.45EE286 pKa = 4.36GFNLDD291 pKa = 3.69GEE293 pKa = 4.79NVLSCSEE300 pKa = 3.96

Molecular weight: 33.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5QZK6|Q5QZK6_IDILO Probable p-aminobenzoyl-glutamate transporter OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) OX=283942 GN=abgT_2 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.36RR14 pKa = 11.84SHH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91VLSAA44 pKa = 4.11

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2608

0

2608

862909

34

1615

330.9

36.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.775 ± 0.048

0.845 ± 0.015

5.78 ± 0.035

6.976 ± 0.047

3.966 ± 0.034

6.911 ± 0.05

2.153 ± 0.026

5.736 ± 0.037

4.989 ± 0.038

10.341 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.023

4.024 ± 0.032

4.005 ± 0.026

4.834 ± 0.048

5.193 ± 0.04

6.493 ± 0.038

5.092 ± 0.03

7.08 ± 0.043

1.362 ± 0.019

2.985 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski