Mycobacterium phage Butters

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Buttersvirus; Mycobacterium virus Butters

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4WNT5|M4WNT5_9CAUD Lysin A OS=Mycobacterium phage Butters OX=1296646 GN=26 PE=4 SV=1
MM1 pKa = 7.27AQAEE5 pKa = 4.32LLDD8 pKa = 3.76TDD10 pKa = 3.62MGTWCPVTLHH20 pKa = 5.79YY21 pKa = 10.61RR22 pKa = 11.84ITDD25 pKa = 3.41GDD27 pKa = 4.34EE28 pKa = 3.56ISYY31 pKa = 10.39LAVTRR36 pKa = 11.84LDD38 pKa = 3.51FVTASGRR45 pKa = 11.84VEE47 pKa = 5.38AFACDD52 pKa = 3.71EE53 pKa = 4.36NGVASTLTPLWVVDD67 pKa = 3.38GDD69 pKa = 4.15MPHH72 pKa = 7.19ADD74 pKa = 3.32ALADD78 pKa = 3.31QGYY81 pKa = 9.23EE82 pKa = 4.08VAA84 pKa = 5.55

Molecular weight: 9.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4W8M4|M4W8M4_9CAUD Tyrosine integrase OS=Mycobacterium phage Butters OX=1296646 GN=37 PE=3 SV=2
MM1 pKa = 7.04TRR3 pKa = 11.84RR4 pKa = 11.84PRR6 pKa = 11.84TYY8 pKa = 10.45LNRR11 pKa = 11.84AQVEE15 pKa = 4.3EE16 pKa = 4.16HH17 pKa = 6.71LGLAVGSLSKK27 pKa = 10.65IKK29 pKa = 10.87LPDD32 pKa = 4.04PDD34 pKa = 4.91VIVGPINDD42 pKa = 5.09DD43 pKa = 3.34GTLPRR48 pKa = 11.84GTVRR52 pKa = 11.84GWTVKK57 pKa = 10.08TIDD60 pKa = 3.19QWNKK64 pKa = 8.3EE65 pKa = 4.14RR66 pKa = 11.84PGRR69 pKa = 11.84GARR72 pKa = 11.84TDD74 pKa = 3.3LRR76 pKa = 11.84HH77 pKa = 5.61

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13288

41

1029

201.3

21.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.973 ± 0.622

1.016 ± 0.166

6.404 ± 0.252

5.908 ± 0.383

2.912 ± 0.186

9.482 ± 0.614

1.972 ± 0.169

4.658 ± 0.204

2.86 ± 0.253

8.03 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.154

2.988 ± 0.209

5.998 ± 0.237

3.492 ± 0.218

7.164 ± 0.391

5.426 ± 0.246

6.502 ± 0.296

6.991 ± 0.272

1.814 ± 0.167

2.348 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski