Malonomonas rubra DSM 5091

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Desulfuromonadaceae; Malonomonas; Malonomonas rubra

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3654 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6IQ36|A0A1M6IQ36_MALRU Multidrug efflux pump subunit AcrB OS=Malonomonas rubra DSM 5091 OX=1122189 GN=SAMN02745165_02201 PE=4 SV=1
MM1 pKa = 7.95KK2 pKa = 10.19YY3 pKa = 10.29NPLIIIFGMLLLLPQVGYY21 pKa = 10.01SAQDD25 pKa = 3.28SYY27 pKa = 11.76IGDD30 pKa = 3.53TAIYY34 pKa = 9.63GGASVTLQPNVLIVFDD50 pKa = 4.55TSGSMDD56 pKa = 4.13DD57 pKa = 4.44EE58 pKa = 6.12IDD60 pKa = 3.9VQVCQPDD67 pKa = 3.46VDD69 pKa = 4.01SDD71 pKa = 4.19GVIDD75 pKa = 5.33EE76 pKa = 5.05SDD78 pKa = 3.55NCPTVANATQTDD90 pKa = 3.64TDD92 pKa = 3.52NDD94 pKa = 5.21GIGDD98 pKa = 4.11LCDD101 pKa = 4.97DD102 pKa = 3.86NTTFPDD108 pKa = 3.48TDD110 pKa = 3.78GDD112 pKa = 4.38GVTDD116 pKa = 3.81DD117 pKa = 4.25TDD119 pKa = 3.55NCKK122 pKa = 8.94VTSNADD128 pKa = 3.37QADD131 pKa = 3.67SDD133 pKa = 4.2GDD135 pKa = 4.34GIGDD139 pKa = 3.59ACEE142 pKa = 4.18VGSGGYY148 pKa = 10.13NKK150 pKa = 9.63DD151 pKa = 2.93TDD153 pKa = 4.07YY154 pKa = 11.0TAMTSSEE161 pKa = 4.09FCGDD165 pKa = 2.84GGGRR169 pKa = 11.84RR170 pKa = 11.84DD171 pKa = 3.9PPWAAEE177 pKa = 3.63ACQRR181 pKa = 11.84NKK183 pKa = 10.12VYY185 pKa = 10.57RR186 pKa = 11.84CRR188 pKa = 11.84DD189 pKa = 3.76DD190 pKa = 4.96EE191 pKa = 4.04WDD193 pKa = 3.47QNGFCSQWDD202 pKa = 3.46RR203 pKa = 11.84VEE205 pKa = 4.62NIDD208 pKa = 5.63YY209 pKa = 11.24SDD211 pKa = 3.85IDD213 pKa = 4.65CNDD216 pKa = 3.16QEE218 pKa = 5.2DD219 pKa = 4.12ALSEE223 pKa = 4.01QGTLVDD229 pKa = 5.05DD230 pKa = 4.01VRR232 pKa = 11.84FDD234 pKa = 4.08EE235 pKa = 6.24DD236 pKa = 4.47GNCDD240 pKa = 3.08GSRR243 pKa = 11.84SSRR246 pKa = 11.84YY247 pKa = 8.66YY248 pKa = 10.32ATGNWVDD255 pKa = 3.47WYY257 pKa = 11.3SLVTIGDD264 pKa = 3.7AGGGASGSGDD274 pKa = 3.79DD275 pKa = 4.27YY276 pKa = 11.82NSGSTQICSTTQEE289 pKa = 4.13RR290 pKa = 11.84KK291 pKa = 9.9NDD293 pKa = 3.26VAEE296 pKa = 4.22NVVSDD301 pKa = 5.01LIEE304 pKa = 4.13STGGVNFGIMRR315 pKa = 11.84FNSSSGGTFMTVSVDD330 pKa = 3.21GQNYY334 pKa = 6.38EE335 pKa = 4.25TTIKK339 pKa = 10.99NMDD342 pKa = 4.45EE343 pKa = 3.8IHH345 pKa = 6.66TGTTTNRR352 pKa = 11.84EE353 pKa = 4.1ALVDD357 pKa = 3.47IASNMPASGYY367 pKa = 8.6TPLGEE372 pKa = 4.29TLYY375 pKa = 11.0EE376 pKa = 3.85AMRR379 pKa = 11.84YY380 pKa = 8.82FSGGNKK386 pKa = 9.18YY387 pKa = 9.91FAGSGSYY394 pKa = 9.29TSPITASCQPNYY406 pKa = 9.85IILMTDD412 pKa = 2.98GMATSDD418 pKa = 3.5SSVPSLSCGGDD429 pKa = 3.54DD430 pKa = 5.91NVDD433 pKa = 3.43CDD435 pKa = 4.04GDD437 pKa = 3.98GRR439 pKa = 11.84SDD441 pKa = 5.31DD442 pKa = 4.75GDD444 pKa = 4.51DD445 pKa = 5.3DD446 pKa = 5.5SLDD449 pKa = 3.63DD450 pKa = 4.31VAWYY454 pKa = 10.01LYY456 pKa = 9.37NTDD459 pKa = 4.52LSDD462 pKa = 4.65FNGTQNVKK470 pKa = 9.62TYY472 pKa = 10.28TIGFGLGGGDD482 pKa = 3.62SDD484 pKa = 6.07AVDD487 pKa = 4.8LLQDD491 pKa = 3.63TADD494 pKa = 3.77NGQGANAGQGKK505 pKa = 10.03SYY507 pKa = 10.38LASSYY512 pKa = 11.2QEE514 pKa = 3.87LTSALSAIIGEE525 pKa = 4.4VLDD528 pKa = 3.68QSSAFVAPVVPTSPEE543 pKa = 3.6NKK545 pKa = 8.99VYY547 pKa = 10.37SGQRR551 pKa = 11.84IYY553 pKa = 11.24LGFFKK558 pKa = 10.62PQITGDD564 pKa = 2.86WYY566 pKa = 11.33GNLKK570 pKa = 10.55KK571 pKa = 10.77FGLDD575 pKa = 3.29NNGEE579 pKa = 4.23VLDD582 pKa = 4.31KK583 pKa = 11.24NGNEE587 pKa = 3.85ATDD590 pKa = 3.65ANGAFLPEE598 pKa = 4.35SVSYY602 pKa = 10.08WGTATDD608 pKa = 3.43AGNVEE613 pKa = 4.27EE614 pKa = 5.05GGIGGILNNRR624 pKa = 11.84VLSTRR629 pKa = 11.84NIYY632 pKa = 9.94TYY634 pKa = 10.59TGTTAALTDD643 pKa = 3.65SSNALTTSNTALTFADD659 pKa = 4.13FDD661 pKa = 3.94VASDD665 pKa = 3.58VRR667 pKa = 11.84KK668 pKa = 10.61DD669 pKa = 3.47EE670 pKa = 4.69IINYY674 pKa = 8.93VYY676 pKa = 10.7GYY678 pKa = 10.34DD679 pKa = 3.34VYY681 pKa = 11.25GINPSVGRR689 pKa = 11.84EE690 pKa = 3.7WVMGDD695 pKa = 3.06ILHH698 pKa = 6.65SKK700 pKa = 9.78PAIQTYY706 pKa = 10.4NSYY709 pKa = 11.15ALSSEE714 pKa = 3.97ADD716 pKa = 3.44PNVNKK721 pKa = 9.58TVIYY725 pKa = 10.43VGSNDD730 pKa = 3.73GQLHH734 pKa = 6.35AFSDD738 pKa = 4.39ANGSEE743 pKa = 4.25LWSFVPPSVLPNLQNLGNNAIHH765 pKa = 7.02EE766 pKa = 4.43YY767 pKa = 11.07FMDD770 pKa = 5.09GSPALYY776 pKa = 10.65VFDD779 pKa = 4.62YY780 pKa = 11.25DD781 pKa = 3.57GDD783 pKa = 4.3GNIGPGPEE791 pKa = 4.64SGDD794 pKa = 3.46TDD796 pKa = 3.68PTGVTDD802 pKa = 5.13AGANDD807 pKa = 3.91KK808 pKa = 11.22VIMVVSMRR816 pKa = 11.84RR817 pKa = 11.84GGGIDD822 pKa = 3.4TLDD825 pKa = 3.61AATSRR830 pKa = 11.84GSYY833 pKa = 9.5YY834 pKa = 11.06ALDD837 pKa = 3.66VTNPLSPQFLWEE849 pKa = 4.12IDD851 pKa = 3.48STTTGFSEE859 pKa = 5.72LGEE862 pKa = 4.3TWSDD866 pKa = 3.11AVIGKK871 pKa = 8.97IRR873 pKa = 11.84LNGSDD878 pKa = 4.54KK879 pKa = 10.5IVAFIGAGYY888 pKa = 10.52DD889 pKa = 3.37NNEE892 pKa = 4.29DD893 pKa = 3.46LRR895 pKa = 11.84FGNTQLFPDD904 pKa = 3.93NTTTATSTILPTADD918 pKa = 3.67ANDD921 pKa = 3.74VTSSGSSAQVNPKK934 pKa = 10.01GRR936 pKa = 11.84GIYY939 pKa = 9.97VIEE942 pKa = 4.58LATVSYY948 pKa = 8.13PTISPFLPTVAVNTSPQKK966 pKa = 9.68LWEE969 pKa = 4.1YY970 pKa = 11.25VYY972 pKa = 11.13DD973 pKa = 4.61ADD975 pKa = 6.28RR976 pKa = 11.84EE977 pKa = 4.17DD978 pKa = 4.49SNLDD982 pKa = 3.44AYY984 pKa = 11.0DD985 pKa = 4.0PGNNPKK991 pKa = 10.12YY992 pKa = 10.9SFVATIATLDD1002 pKa = 3.7SDD1004 pKa = 3.69FDD1006 pKa = 4.39GYY1008 pKa = 10.54IDD1010 pKa = 4.01RR1011 pKa = 11.84LYY1013 pKa = 11.31SGDD1016 pKa = 3.19TGGNMWRR1023 pKa = 11.84FDD1025 pKa = 3.66IGDD1028 pKa = 3.83KK1029 pKa = 10.44TSTSAWDD1036 pKa = 3.26ATKK1039 pKa = 10.33IFSANPSDD1047 pKa = 3.98VANSDD1052 pKa = 3.68EE1053 pKa = 4.96DD1054 pKa = 3.88PATNGRR1060 pKa = 11.84KK1061 pKa = 8.97IFYY1064 pKa = 9.69RR1065 pKa = 11.84PSIVQEE1071 pKa = 3.75PGYY1074 pKa = 10.27VGVYY1078 pKa = 10.29FGTGDD1083 pKa = 3.7RR1084 pKa = 11.84AHH1086 pKa = 7.5PLNKK1090 pKa = 10.09AVIDD1094 pKa = 3.82RR1095 pKa = 11.84LYY1097 pKa = 11.19AVYY1100 pKa = 10.54DD1101 pKa = 3.6RR1102 pKa = 11.84GNITSARR1109 pKa = 11.84TEE1111 pKa = 3.79EE1112 pKa = 4.18HH1113 pKa = 6.13LVNVTEE1119 pKa = 5.89DD1120 pKa = 3.73NLQSASPGIGVVNSTLLALSSTSNYY1145 pKa = 9.75GWFIKK1150 pKa = 10.59LDD1152 pKa = 3.84LLDD1155 pKa = 4.14GEE1157 pKa = 5.2KK1158 pKa = 10.72VLSNPIVFNKK1168 pKa = 8.58VAYY1171 pKa = 7.71YY1172 pKa = 7.36TTFTANVPSDD1182 pKa = 3.99DD1183 pKa = 3.98PCEE1186 pKa = 3.85QGQLGIGRR1194 pKa = 11.84LYY1196 pKa = 11.01AVDD1199 pKa = 4.01YY1200 pKa = 8.34KK1201 pKa = 10.91TGEE1204 pKa = 3.87AVFNFDD1210 pKa = 4.13LTNDD1214 pKa = 3.45VADD1217 pKa = 3.89EE1218 pKa = 4.15DD1219 pKa = 4.98SYY1221 pKa = 12.26AEE1223 pKa = 4.13NEE1225 pKa = 4.12NEE1227 pKa = 3.82RR1228 pKa = 11.84AIAGGGKK1235 pKa = 9.14ILRR1238 pKa = 11.84RR1239 pKa = 11.84SDD1241 pKa = 3.2RR1242 pKa = 11.84VKK1244 pKa = 10.21TIGSGIPSGAVVVVRR1259 pKa = 11.84EE1260 pKa = 4.78DD1261 pKa = 3.6GTSSALIGCGGGLCGGEE1278 pKa = 4.14TKK1280 pKa = 10.81GGGTAVPIYY1289 pKa = 10.74WMMEE1293 pKa = 3.59

Molecular weight: 138.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6JW85|A0A1M6JW85_MALRU Uncharacterized protein OS=Malonomonas rubra DSM 5091 OX=1122189 GN=SAMN02745165_02541 PE=4 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.43PKK5 pKa = 9.39RR6 pKa = 11.84TYY8 pKa = 8.97QPSRR12 pKa = 11.84IKK14 pKa = 10.52RR15 pKa = 11.84KK16 pKa = 8.04RR17 pKa = 11.84THH19 pKa = 6.13GFRR22 pKa = 11.84KK23 pKa = 10.09RR24 pKa = 11.84MQTKK28 pKa = 10.03SGRR31 pKa = 11.84LVINRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84SRR40 pKa = 11.84GRR42 pKa = 11.84KK43 pKa = 8.35SLSVTTPQKK52 pKa = 11.05

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3654

0

3654

1195547

40

4834

327.2

36.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.793 ± 0.059

1.263 ± 0.022

5.597 ± 0.046

6.909 ± 0.042

4.106 ± 0.033

7.427 ± 0.045

2.003 ± 0.024

6.004 ± 0.04

5.185 ± 0.039

11.155 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.46 ± 0.024

3.485 ± 0.029

4.254 ± 0.029

4.45 ± 0.04

5.433 ± 0.041

5.999 ± 0.047

4.817 ± 0.039

6.854 ± 0.032

1.04 ± 0.014

2.766 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski