Lake Sarah-associated circular virus-23

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA99|A0A126GA99_9VIRU Coat protein OS=Lake Sarah-associated circular virus-23 OX=1685750 PE=4 SV=1
MM1 pKa = 7.01ATSVPVTSVPGKK13 pKa = 9.09MRR15 pKa = 11.84NVCFTLNNYY24 pKa = 8.45TEE26 pKa = 4.91DD27 pKa = 3.31DD28 pKa = 4.06CNRR31 pKa = 11.84LRR33 pKa = 11.84TLEE36 pKa = 4.23KK37 pKa = 10.58GVGYY41 pKa = 10.16LIFAKK46 pKa = 10.36EE47 pKa = 4.32VGDD50 pKa = 3.59SGTPHH55 pKa = 5.93LQGYY59 pKa = 9.56IEE61 pKa = 4.38FEE63 pKa = 4.3NPRR66 pKa = 11.84SPGKK70 pKa = 9.67DD71 pKa = 2.22WKK73 pKa = 10.9NFKK76 pKa = 10.56EE77 pKa = 4.2LMGGCHH83 pKa = 5.6FEE85 pKa = 4.09RR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84GTAKK92 pKa = 10.11QASDD96 pKa = 3.63YY97 pKa = 11.43CEE99 pKa = 3.78FADD102 pKa = 5.67YY103 pKa = 7.73PTCQIKK109 pKa = 10.81NEE111 pKa = 3.94EE112 pKa = 4.28VYY114 pKa = 10.84KK115 pKa = 10.73FGEE118 pKa = 4.01ISRR121 pKa = 11.84QGDD124 pKa = 3.47RR125 pKa = 11.84TDD127 pKa = 2.78WAQAVNQIIGEE138 pKa = 4.06KK139 pKa = 10.17RR140 pKa = 11.84EE141 pKa = 4.07VVDD144 pKa = 5.45VIMDD148 pKa = 3.72QPQLLPAIRR157 pKa = 11.84ALEE160 pKa = 4.04NFKK163 pKa = 11.18KK164 pKa = 8.16MTIQPIEE171 pKa = 4.56RR172 pKa = 11.84EE173 pKa = 4.2VHH175 pKa = 4.24VTWLHH180 pKa = 5.94GSPGSGKK187 pKa = 7.66TRR189 pKa = 11.84WVWDD193 pKa = 3.96KK194 pKa = 11.39YY195 pKa = 10.3PEE197 pKa = 4.44VYY199 pKa = 10.5SKK201 pKa = 11.07PSGNWWDD208 pKa = 3.99GYY210 pKa = 11.12NGEE213 pKa = 4.42EE214 pKa = 4.11TLLLDD219 pKa = 5.93DD220 pKa = 4.8YY221 pKa = 12.09DD222 pKa = 4.34EE223 pKa = 6.07DD224 pKa = 4.24IPFAEE229 pKa = 4.52LLKK232 pKa = 11.18VLDD235 pKa = 4.13RR236 pKa = 11.84YY237 pKa = 10.51KK238 pKa = 10.98YY239 pKa = 9.17RR240 pKa = 11.84VPVKK244 pKa = 10.44GGFVGARR251 pKa = 11.84WTRR254 pKa = 11.84VFITTNNPPVSFYY267 pKa = 10.88KK268 pKa = 10.7YY269 pKa = 9.74VSNRR273 pKa = 11.84SALDD277 pKa = 3.46RR278 pKa = 11.84RR279 pKa = 11.84IHH281 pKa = 6.39EE282 pKa = 4.18IKK284 pKa = 10.91CFDD287 pKa = 3.64EE288 pKa = 4.5VV289 pKa = 3.4

Molecular weight: 33.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA99|A0A126GA99_9VIRU Coat protein OS=Lake Sarah-associated circular virus-23 OX=1685750 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.7YY3 pKa = 11.02NMPRR7 pKa = 11.84PRR9 pKa = 11.84ARR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.28PRR17 pKa = 11.84ARR19 pKa = 11.84RR20 pKa = 11.84SRR22 pKa = 11.84RR23 pKa = 11.84PRR25 pKa = 11.84ANPRR29 pKa = 11.84NVAEE33 pKa = 4.22WASMSCKK40 pKa = 8.37QTIISTAGTDD50 pKa = 3.92FQCNNMYY57 pKa = 11.05GLVGVIKK64 pKa = 9.75LTGYY68 pKa = 9.67LRR70 pKa = 11.84AMEE73 pKa = 4.15VAKK76 pKa = 10.41AYY78 pKa = 10.04QFYY81 pKa = 10.4RR82 pKa = 11.84IKK84 pKa = 10.53SVKK87 pKa = 8.51ITYY90 pKa = 9.33KK91 pKa = 10.49FPFDD95 pKa = 3.46TFVPGNVARR104 pKa = 11.84PNFYY108 pKa = 10.92YY109 pKa = 10.09IVDD112 pKa = 3.74KK113 pKa = 11.23ARR115 pKa = 11.84AISPLITLEE124 pKa = 3.97SLKK127 pKa = 10.93EE128 pKa = 3.62MGARR132 pKa = 11.84PRR134 pKa = 11.84QCDD137 pKa = 3.53EE138 pKa = 4.41KK139 pKa = 10.59PISVTWAPSVLQDD152 pKa = 3.16VDD154 pKa = 4.27SVGGAQPAQYY164 pKa = 10.5NISPWLSTAHH174 pKa = 6.06QDD176 pKa = 3.48VNHH179 pKa = 6.97EE180 pKa = 4.06GLVWYY185 pKa = 10.25AEE187 pKa = 3.92QVLFGGTAQIYY198 pKa = 9.31HH199 pKa = 7.11AEE201 pKa = 4.18LEE203 pKa = 4.33LQFEE207 pKa = 4.6FKK209 pKa = 10.62KK210 pKa = 10.23PQLYY214 pKa = 6.78PTAVEE219 pKa = 4.52GGAPLTSMLYY229 pKa = 10.35AKK231 pKa = 10.36SAVRR235 pKa = 11.84DD236 pKa = 3.65NSSNGIVDD244 pKa = 5.02GIAA247 pKa = 3.19

Molecular weight: 27.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

536

247

289

268.0

30.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.09 ± 2.301

1.679 ± 0.311

5.224 ± 1.058

6.343 ± 1.265

4.104 ± 0.308

6.903 ± 0.556

1.493 ± 0.186

5.037 ± 0.151

6.343 ± 0.452

5.97 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.398

4.664 ± 0.141

6.157 ± 0.486

3.918 ± 0.63

7.276 ± 0.279

5.41 ± 0.714

5.224 ± 0.245

7.649 ± 0.242

2.239 ± 0.415

5.037 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski