Proteus phage PM 85

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Acadevirus; Proteus virus PM85

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6NYA6|A0A0F6NYA6_9CAUD Uncharacterized protein OS=Proteus phage PM 85 OX=1560283 GN=PM85_009 PE=4 SV=1
MM1 pKa = 7.51AFEE4 pKa = 4.52IVDD7 pKa = 3.8TEE9 pKa = 4.55TTQDD13 pKa = 3.02TSTTTEE19 pKa = 3.39VDD21 pKa = 3.33TQEE24 pKa = 4.44VKK26 pKa = 10.79DD27 pKa = 4.06VNNNDD32 pKa = 3.41NTDD35 pKa = 3.38TGSTDD40 pKa = 3.83DD41 pKa = 3.82VQGNPAPEE49 pKa = 4.38GDD51 pKa = 3.98EE52 pKa = 4.03NSGGEE57 pKa = 4.05DD58 pKa = 2.87TGGNGQDD65 pKa = 5.11DD66 pKa = 4.0KK67 pKa = 11.75QEE69 pKa = 4.15PQGTEE74 pKa = 3.28DD75 pKa = 2.86STYY78 pKa = 9.7YY79 pKa = 10.68FGGNEE84 pKa = 3.97VEE86 pKa = 5.01IEE88 pKa = 3.94IPQDD92 pKa = 3.35VEE94 pKa = 3.88EE95 pKa = 4.28SLKK98 pKa = 11.07EE99 pKa = 3.8KK100 pKa = 10.94GIDD103 pKa = 3.37AKK105 pKa = 11.06EE106 pKa = 3.88LVAEE110 pKa = 5.19LYY112 pKa = 11.06SKK114 pKa = 11.21DD115 pKa = 3.46GDD117 pKa = 3.67FSLSDD122 pKa = 3.23EE123 pKa = 4.73TKK125 pKa = 10.55QKK127 pKa = 11.03LYY129 pKa = 11.18DD130 pKa = 3.63AFGKK134 pKa = 10.22FAVDD138 pKa = 4.34AYY140 pKa = 11.39LSGLKK145 pKa = 9.87AQNEE149 pKa = 4.3MFFMNEE155 pKa = 3.17ATKK158 pKa = 10.64AKK160 pKa = 10.33EE161 pKa = 3.88MEE163 pKa = 4.04QANAQRR169 pKa = 11.84FNDD172 pKa = 3.24IAKK175 pKa = 10.16EE176 pKa = 4.04VGGDD180 pKa = 3.66EE181 pKa = 3.93GWTRR185 pKa = 11.84LEE187 pKa = 3.93EE188 pKa = 4.25FALSTLSNEE197 pKa = 3.9EE198 pKa = 3.66LTAFNAVMDD207 pKa = 4.39SGNQYY212 pKa = 10.08LQMYY216 pKa = 9.16AVRR219 pKa = 11.84EE220 pKa = 3.95LEE222 pKa = 3.91ARR224 pKa = 11.84RR225 pKa = 11.84KK226 pKa = 8.36QAQGDD231 pKa = 4.02DD232 pKa = 3.77KK233 pKa = 11.86VEE235 pKa = 4.04LVQGTPASDD244 pKa = 3.55EE245 pKa = 4.38GDD247 pKa = 3.55NSPLSAQEE255 pKa = 4.01YY256 pKa = 8.47IRR258 pKa = 11.84EE259 pKa = 4.07VAQIGSKK266 pKa = 10.47FRR268 pKa = 11.84GDD270 pKa = 2.9RR271 pKa = 11.84KK272 pKa = 10.44GAAEE276 pKa = 4.07YY277 pKa = 9.7QAKK280 pKa = 10.38LDD282 pKa = 3.46ARR284 pKa = 11.84RR285 pKa = 11.84RR286 pKa = 11.84AGMARR291 pKa = 11.84GLL293 pKa = 3.78

Molecular weight: 32.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6NYI6|A0A0F6NYI6_9CAUD DNA ligase OS=Proteus phage PM 85 OX=1560283 GN=PM85_0010 PE=4 SV=1
MM1 pKa = 6.98YY2 pKa = 9.98QLKK5 pKa = 10.68VNIGSRR11 pKa = 11.84VRR13 pKa = 11.84NIHH16 pKa = 5.47KK17 pKa = 10.35HH18 pKa = 3.97SVNYY22 pKa = 9.73GKK24 pKa = 10.47SGFIEE29 pKa = 4.56RR30 pKa = 11.84EE31 pKa = 3.98VLGVKK36 pKa = 9.59PSKK39 pKa = 10.18WLVRR43 pKa = 11.84YY44 pKa = 9.96NDD46 pKa = 4.28GSCGTYY52 pKa = 10.26FKK54 pKa = 10.0HH55 pKa = 5.45TAHH58 pKa = 7.55RR59 pKa = 11.84SLQVVDD65 pKa = 4.62EE66 pKa = 4.42YY67 pKa = 11.71KK68 pKa = 10.58SVKK71 pKa = 8.11QTIKK75 pKa = 10.04EE76 pKa = 3.99QQKK79 pKa = 9.22EE80 pKa = 3.94EE81 pKa = 4.45RR82 pKa = 11.84IMIKK86 pKa = 9.77RR87 pKa = 11.84EE88 pKa = 3.49RR89 pKa = 11.84RR90 pKa = 11.84NVITGWTQSEE100 pKa = 4.06LMKK103 pKa = 10.61RR104 pKa = 11.84HH105 pKa = 5.22GNAMGVVQFNQRR117 pKa = 11.84ALGKK121 pKa = 7.47TTGQAMAVIGSAMVQPYY138 pKa = 10.02VPVSFHH144 pKa = 6.81NIDD147 pKa = 3.33HH148 pKa = 7.35AIFEE152 pKa = 5.11HH153 pKa = 7.05NIPTQVANANMRR165 pKa = 11.84DD166 pKa = 3.82MIKK169 pKa = 10.86DD170 pKa = 4.29LINTLGFKK178 pKa = 10.44GFRR181 pKa = 11.84FNNNNNTIVFNPIVTEE197 pKa = 3.97EE198 pKa = 4.35NYY200 pKa = 10.27VEE202 pKa = 4.6SVV204 pKa = 2.93

Molecular weight: 23.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

10872

72

1273

472.7

52.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.864 ± 0.554

0.92 ± 0.184

6.429 ± 0.252

7.009 ± 0.352

3.909 ± 0.159

7.791 ± 0.294

1.904 ± 0.244

5.85 ± 0.278

6.714 ± 0.267

7.745 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.164 ± 0.224

4.746 ± 0.263

3.339 ± 0.18

4.295 ± 0.289

5.362 ± 0.384

6.025 ± 0.38

5.427 ± 0.441

6.751 ± 0.35

1.38 ± 0.147

3.376 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski